Nivalenol‐producing <i><scp>F</scp>usarium cerealis</i> associated with fusarium head blight in winter wheat in <scp>M</scp>anitoba, <scp>C</scp>anada

Amarasinghe, Chami C.; Tittlemier, Sheryl A.; Fernando, W. G. Dilantha

doi:10.1111/ppa.12329

Cited by 27 publications

(17 citation statements)

References 41 publications

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“…The F. ussurianum strain 29813 representing the 3-ADON chemotype produced the largest amount of DON among all the analyzed cultures. The relation of F. cerealis strains to the NIV chemotype is also in line with the data in [ 68 ]. We believe that the results obtained in the present study can partially compensate for information absent in the European database on the chemotypes of strains from certain regions of Russia.…”

Section: Discussionsupporting

confidence: 89%

Phylogenetic Analysis and Molecular Typing of Trichothecene-Producing Fusarium Fungi from Russian Collections

et al. 2018

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We performed a three-locus phylogenetic analysis of Fusarium strains presumably capable of trichothecene production, which were deposited in the Russian national collections. The intra- and interspecific polymorphism of partial sequences of the translation elongation factor 1 alpha (TEF1α) gene and two genes from the trichothecene cluster TRI5 and TRI14 was studied. A study of 60 strains of different origins using DNA markers confirmed, and in the case for several strains, clarified their taxonomic characteristics. As a result, a strain of F. commune (F-900) was identified in Russia for the first time. Furthermore, the strain F-846 proved to be phylogenetically distinct from any of the known Fusarium species. F. equiseti strains from Northwest Russia were found to belong to the North European group (I), whereas a strain from the North Caucasus – to the South European one (II). Partial TRI14 sequences from 9 out of 12 species were determined for the first time. Their comparative analysis demonstrated a relatively high level of intraspecific variability in F. graminearum and F. sporotrichioides, but no correlation between the sequence polymorphism and the geographic origin of the strains or their chemotype was found. Specific chemotypes of trichothecene B producers were characterized using two primer sets. The chemotyping results were verified by HPLC.

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Section: Discussionsupporting

confidence: 89%

Phylogenetic Analysis and Molecular Typing of Trichothecene-Producing Fusarium Fungi from Russian Collections

et al. 2018

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“…Isolates from 3ADON populations had significantly higher fecundity and growth rates in comparison to isolates from the 15ADON population (Ward et al 2008). A similar pattern was seen in field studies in Canada (Amarasinghe et al 2015;Gilbert et al 2010) and the USA. Aggressiveness, saprophytic fitness and pathogenic fitness were similar between 15ADON and 3ADON chemotypes (Kuhnem et al 2015;Spolti et al 2014).…”

Section: Chemotypessupporting

confidence: 73%

“…The Asian and New World populations are genetically distinct (O'Donnell et al 2000). A noted, nivalenol-producing strains of Fusarium are rare in Canada and the USA (Amarasinghe et al 2015;Gale et al 2011;Tanaka et al 1988), but are more common in Japan and China due to the nivalenol-producing F. asiaticum (Gale et al 2011).…”

Section: Populations In North Americamentioning

confidence: 99%

Comparative Analysis of Fusarium Graminearum Strain Genotype and Chemotype from Grains Sampled During the 2015-2017 Ontario Harvests

Crippin¹

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Fusarium graminearum is a fungal pathogen of small grains and corn resulting in yield loss and more importantly the crops are contaminated with the regulated mycotoxin deoxynivalenol (DON). In recent years, genetic changes in the F. graminearum population have been occurring and potential concerns include the risk of more DON in grain with less visible disease, new toxins and an increase in pathogen fitness and virulence. The aim of this study was to isolate F. graminearum from wheat and corn collected during the 2015-2017 Ontario harvests, and compare genotype and chemotype analysis. From the 2015-2017, 228 corn fields and 118 wheat fields in Ontario were screened for the prevalence of F. graminearum. The genotypes of two known F. graminearum populations were assessed using multiplex PCR targeted to TRI3 and TRI12 gene sequences. F. graminearum isolates from 2015-2017 harvest wheat (n = 308) and corn (n = 160) were identified. Representative strains (n = 61) from each year were further examined using PCR-based assays to determine genotype and chemical analysis to determine chemotype. All wheat and corn isolates were the 15ADON genotype. However, chemical analysis of culture extracts showed co-production of 15ADON and the new trichothecene 3ANX in one or more culture media for 80% (n = 16), 59% (n = 13) and 73.7% (n = 14) for the years 2015, 2016, and 2017 respectively. None of the representative strains were of the 3ANX genotype. These findings demonstrate the existing probes to assess genotype cannot reliably predict chemotype of the population of F. graminearum in Ontario.

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“…These pathogenic species produce a wide range of mycotoxins, such as trichothecenes (TCTs), fumonisins (FBs), zearalenone (ZEN), beauvericin (BEA) and moniliformin (MON), which have been determined to be common contaminants of cereal grains and derived products (Bottalico and Perrone, 2002;Jestoi et al, 2008;Goetz et al, 2010). Among the toxigenic Fusarium species that have been associated with infected grain are Fusarium graminearum, Fusarium culmorum, Fusarium ave naceum, Fusarium cerealis, and Fusarium temperatum (Bottalico and Perrone, 2002;Logrieco et al, 2002a;2002b;Goetz et al, 2010;Amarasinghe et al, 2014;Czembor et al, 2014). F. graminearum is capable of producing two major types of mycotoxins: estrogenic ZEN and type B trichothecenes, such as deoxyniva-lenol (DON) and, depending on the chemotype, its acetylated forms 3-acetyl-deoxynivalenol (3-AcDON) or 15-acetyl-deoxynivalenol (15-AcDON), as well as nivalenol (NIV) (Bily et al, 2004;Glenn, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…The main mycotoxins biosynthesized by F. culmorum include DON, 3-AcDON, NIV, fusarenone X (FUS) and ZEN (Glenn, 2007;Wagacha and Muthomi, 2007). NIV, FUS and ZEN are also formed in cereals by F. cerea lis strains (Logrieco et al, 2003;Amarasinghe et al, 2014). However, F. avenaceum and F. temperatum do not produce TCTs or ZEN, but they do produce other mycotoxins such as enniatins (ENs), BEA and MON (Logrieco et al, 2002b;Jestoi et al, 2008;Scauflaire et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Microbial Biomass and Enzymatic Activity of the Surface Microlayer and Subsurface Water in Two Dystrophic Lakes

Kostrzewska-Szlakowska

Kiersztyn

2017

Polish Journal of Microbiology

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The aim of the present study was to examine the abilities of twenty-four isolates belonging to ten different Trichoderma species (i.e., Trichoderma atroviride, Trichoderma citrinoviride, Trichoderma cremeum, Trichoderma hamatum, Trichoderma harzianum, Trichoderma koningiopsis, Trichoderma longibrachiatum, Trichoderma longipile, Trichoderma viride and Trichoderma viridescens) to inhibit the mycelial growth and mycotoxin production by five Fusarium strains (i.e., Fusarium avenaceum, Fusarium cerealis, Fusarium culmorum, Fusarium graminearum and Fusarium temperatum). Dual-culture bioassay on potato dextrose agar (PDA) medium clearly documented that all of the Trichoderma strains used in the study were capable of influencing the mycelial growth of at least four of all five Fusarium species on the fourth day after co-inoculation, when there was the first apparent physical contact between antagonist and pathogen. The qualitative evaluation of the interaction between the colonies after 14 days of co-culturing on PDA medium showed that ten Trichoderma strains completely overgrew and sporulated on the colony at least one of the tested Fusarium species. Whereas, the microscopic assay provided evidence that only T. atroviride AN240 and T. viride AN255 formed dense coils around the hyphae of the pathogen from where penetration took place. Of all screened Trichoderma strains, T. atroviride AN240 was also found to be the most efficient (69-100% toxin reduction) suppressors of mycotoxins (deoxynivalenol, 3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol, nivalenol, zearalenone, beauvericin, moniliformin) production by all five Fusarium species on solid substrates. This research suggests that T. atroviride AN240 can be a promising candidate for the biological control of toxigenic Fusarium species.

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Nivalenol‐producing Fusarium cerealis associated with fusarium head blight in winter wheat in Manitoba, Canada

Cited by 27 publications

References 41 publications

Phylogenetic Analysis and Molecular Typing of Trichothecene-Producing Fusarium Fungi from Russian Collections

Phylogenetic Analysis and Molecular Typing of Trichothecene-Producing Fusarium Fungi from Russian Collections

Comparative Analysis of Fusarium Graminearum Strain Genotype and Chemotype from Grains Sampled During the 2015-2017 Ontario Harvests

Microbial Biomass and Enzymatic Activity of the Surface Microlayer and Subsurface Water in Two Dystrophic Lakes

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