NK cell predicts the severity of acute graft-versus-host disease in patients after allogeneic stem cell transplantation using antithymocyte globulin (ATG) in pretreatment scheme

Abstract

BackgroundGraft-versus-host disease (GVHD) is one of the most complex complications after allogeneic stem cell transplantation. Current standard of grading system is based on clinical symptoms in skin, liver and intestinal. However, it’s difficult to differ GVHD and its extent just by clinical manifestation. Here we retrospectively analyzed cell immune function in patients implemented allogeneic stem cell transplantation in Ningbo first Hospital from Jan 2013 to Jan 2018.Resultsthe data are collected from 51 patients (mean age was 42; 45.1% women). The average NK cell percentage was 39.31% in severe GVHD (Grade III-IV), was 16.98% in mild GVHD (GradeI-II), while was 21.15% in No GVHD group. The statistical analysis showed difference among each grade. Further analysis was performed in Antithymocyte globulin (ATG) treated group and control group. We showed NK Cell percentage was sharply different in ATG treated group: 47.34% in severe GVHD, 11.98% in mild GVHD group, while 18.3% in no GVHD group. However, in control group, the average percentage of NK cells was 23.27% in severe GVHD, was 23.22%in mild GVHD group, while was 21.13% in no GVHD group.ConclusionThe data supports that ATG can prevent GVHD by increasing NK cell percentage. The percentage of NK cell seemed to be a useful probe to evaluate the severity of GVHD in allogeneic stem cell transplantation patients using ATG in pretreatment.

The add-on effects of Danhong injection among patients with ischemic stroke receiving Western medicines: A systematic review and meta-analysis

“…These 67 RCTs ( Liu and Huang 2010 ; Xue eand Li, 2010 ; Ren et al, 2011 ; Luo et al, 2012 ; Shen, 2012 ; Su et al, 2012 ; Zou et al, 2013 ; Liang et al, 2014 ; Song and Yang 2014 ; Yin, 2014 ; Fan et al, 2015 ; Li, 2015 ; Ma et al, 2015 ; Zhang, 2015 ; Cao and Mei 2016 ; Feng et al, 2016 ; Wang, 2016 ; Wang et al, 2016 ; Wu and Bao 2016 ; Zeng et al, 2016 ; Li Q. et al, 2017 ; Liu F. et al, 2017 ; Li Y. et al, 2017 ; Liu Z. et al, 2017 ; Chen, 2017 ; Chen and Shi., 2017 ; Li, 2017 ; Liu, 2017 ; Ma, 2017 ; Ou and Liu 2017 ; Pen, 2017 ; Qiu et al, 2017 ; Wei et al, 2017 ; Yun et al, 2017 ; Zhang, 2017 ; Yang and Liu 2018a ; Yang et al, 2018b ; Dai, 2018 ; Fan et al, 2018 ; Ge, 2018 ; Li et al, 2018 ; Liu, 2018 ; Luo, 2018 ; Lv et al, 2018 ; Shi, 2018 ; Yang, 2018 ; Zhang et al, 2018 ; Liu Q. et al, 2019 ; Cao et al, 2019 ; Chai et al, 2019 ; Chen et al, 2019 ; Cheng, 2019 ; Liu Y. et al, 2019 ; Deng, 2019 ; Jin et al, 2019 ; Li and Tian 2019 ; Yuan, 2019 ; Zhang, 2019 ; Cao, 2020 ; Huo et al, 2020 ;…”

“…Of the 67 included trials, 16 studies ( Liu and Huang 2010 ; Shen, 2012 ; Wang, 2016 ; Li Q. et al, 2017 ; Li Y. et al, 2017 ; Chen and Shi, 2017 ; Wei et al, 2017 ; Zhang, 2017 ; Yang ad Liu 2018a ; Ge, 2018 ; Li et al, 2018 ; Liu, 2018 ; Lv et al, 2018 ; Zhang et al, 2018 ; Li and Tian, 2019 ; Zhuang and Zhao, 2020 ) reported at least one case of ADRs, 12 studies ( Xue and Li, 2010 ; Su et al, 2012 ; Song and yang, 2014 ; Cao and Mei, 2016 ; Wu and Bao, 2016 ; Luo, 2018 ; Cao et al, 2019 ; Cheng, 2019 ; Zhang, 2019 ; Cao, 2020 ; Jiang et al, 2020 ; Kang et al, 2020 ) reported no ADRs/ADEs during the study, and 39 RCTs did not mention the information of ADRs/ADEs.…”

NK cell alloreactivity in acute myeloid leukemia in the post‐transplant cyclophosphamide era

“…43,44 Though NK cell reconstitution after ATG seems to be more rapid than without the use of ATG, studies concerning NK cell function in this setting are scarce and conflicting. 37,45,46 of grade I-II aGvHD of 54%, grade III-IV aGvHD of 4% and without development of cGvHD. One patient developed severe acute GvHD but received stem cells from an older female donor, known to be associated with highest incidence of aGvHD in haploSCT.…”

“…Moreover, in vivo and in vitro studies show that NK cells are also sensitive to ATG, and NK cell numbers drop dramatically after ATG administration 43,44 . Though NK cell reconstitution after ATG seems to be more rapid than without the use of ATG, studies concerning NK cell function in this setting are scarce and conflicting 37,45,46 . Differences in the effect of KIR‐LM after PTCy between the different studies might be due to differences in the conditioning regimen and stem cell source, resulting in different numbers of alloreactive T cells present in the graft.…”

Single-Nucleotide Polymorphisms in MICA and MICB Genes Could Play a Role in the Outcome in AML Patients after HSCT

“…Natural killer (NK) cells are the first lymphocytes reaching the standard numbers within the first few weeks post-transplantation [2], and, therefore, they play a key role in hematopoiesis during the first months after HSCT. NK cells are recognized as crucial cells for early relapse control, and recent data have demonstrated that NK cells also affect GVHD development [3][4][5]. The exact role of NK cells and crucial parameters, which could help us to improve the outcome of HSCT, are currently broadly investigated.…”