NLRP2 is highly expressed in a mouse model of ischemic stroke

Sun, Xia; Song, Xuemeng; Zhang, Li; Sun, Jian; Wei, Xinbing; Meng, Liya; An, Jie

doi:10.1016/j.bbrc.2016.09.157

Cited by 35 publications

(27 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, the NLRP1 and the NLRP3 inflammasome have been shown to be activated by thromboembolic stroke (Abulafia et al, 2009 ; Fann et al, 2013 ). The NLRP2 inflammasome, which was initially described in human astrocytes (Minkiewicz et al, 2013 ), also contributes to the inflammatory response after cerebral ischemia in mice (Sun et al, 2016 ). Following intracerebral hemorrhage, the NLRP3 inflammasome plays a role in the infiltration of neutrophils (Ma et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Inflammasome Proteins in Serum and Serum-Derived Extracellular Vesicles as Biomarkers of Stroke

Kerr

Garcia‐Contreras

Abbassi

et al. 2018

Front. Mol. Neurosci.

View full text Add to dashboard Cite

The inflammasome is a key contributor to the inflammatory innate immune response after stroke. We have previously shown that inflammasome proteins are released in extracellular vesicles (EV) after brain and spinal cord injury. In addition, we have shown that inflammasome proteins offer great promise as biomarkers of central nervous system (CNS) injury following brain trauma. In the present study, we used a Simple Plex Assay (Protein Simple), a novel multi-analyte automated microfluidic immunoassay platform, to analyze serum and serum-derived EV samples from stroke patients and control subjects for inflammasome protein levels of caspase-1, apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC), Interleukins (IL)-1β, and (IL)-18. Receiver operator characteristic (ROC) curves with associated confidence intervals obtained from the analysis of serum samples revealed that the area under the curve (AUC) for ASC was 0.99 with a confidence interval between 0.9914 and 1.004, whereas the AUC for caspase-1, IL-1β, and IL-18 were 0.75, 0.61, and 0.67, respectively. Thus, these data indicate that ASC is a potential biomarker of stroke and highlight the role of the inflammasome in the inflammatory response after brain ischemia.

show abstract

Section: Discussionmentioning

confidence: 99%

Inflammasome Proteins in Serum and Serum-Derived Extracellular Vesicles as Biomarkers of Stroke

Kerr

Garcia‐Contreras

Abbassi

et al. 2018

Front. Mol. Neurosci.

View full text Add to dashboard Cite

show abstract

“…35 Later, the presence NLRP2 inflammasomes in the CNS, where they are mainly found in astrocytes, 36 led to the discovery of their role in stroke. For example, the role of NLRP2 in cerebral ischemia has been reported by Sun et al, 32 who reported increased expression of NLRP2 protein both in vitro and in vivo models of ischemic stroke, and a reduction in apoptosis via silencing of the NLRP2 gene. 32 NLRC4, also known as IPAF, is reported to be involved in cerebral ischemia via its response to DAMPs that are associated with neuroinflammation; 37 Denes et al reported a reduction in neuronal injury in ASC and NLRC4 knockout mice.…”

Section: Inflammasomes and Progression Of Strokementioning

confidence: 82%

“…For example, the role of NLRP2 in cerebral ischemia has been reported by Sun et al, 32 who reported increased expression of NLRP2 protein both in vitro and in vivo models of ischemic stroke, and a reduction in apoptosis via silencing of the NLRP2 gene. 32 NLRC4, also known as IPAF, is reported to be involved in cerebral ischemia via its response to DAMPs that are associated with neuroinflammation; 37 Denes et al reported a reduction in neuronal injury in ASC and NLRC4 knockout mice. 37 The AIM2-containing inflammasome, which responds to DNA, is involved in pyroptotic cell death by utilizing its ASC domain during cerebral ischemia.…”

Section: Inflammasomes and Progression Of Strokementioning

confidence: 82%

“…Later, the presence NLRP2 inflammasomes in the CNS, where they are mainly found in astrocytes, led to the discovery of their role in stroke. For example, the role of NLRP2 in cerebral ischemia has been reported by Sun et al , who reported increased expression of NLRP2 protein both in vitro and in vivo models of ischemic stroke, and a reduction in apoptosis via silencing of the NLRP2 gene …”

Section: Inflammasomes and Progression Of Strokementioning

confidence: 83%

“…The four most commonly involved inflammasomes in the inflammatory progression following stroke are those that contain NLRP1, NLRP3, AIM2, or NLRC4 PRRs, 31 though NLRP2 is also involved to some extent. 32 NLRP3 and NLRC4 are mainly expressed in microglia and endothelial cells, whereas AIM2 inflammasomes are expressed in neurons. 31 NLRP1 was the first inflammasome to be discovered in neurons and astrocytes, specifically in microglia and is upregulated within 6 h following stroke.…”

Section: Inflammasomes and Progression Of Strokementioning

confidence: 99%

See 2 more Smart Citations

Inflammasomes in stroke: a triggering role for acid‐sensing ion channels

Vats

Sarmah

Kaur

et al. 2018

Annals of the New York Academy of Sciences

View full text Add to dashboard Cite

Stroke is devastating and a major cause of morbidity and mortality around the world. The innate immune response plays an important role in various brain injuries, including stroke, and targeting it for therapeutic interventions would likely prove beneficial. The panoply of inflammatory cells, which induce various cellular, hormonal, and biochemical alterations, mediates the rapid progression of injury in stroke. The inflammasome, a multiprotein oligomer and a key component of specific innate immune responses, contributes toward the worsening of stroke outcomes by activating inflammatory cytokines. Acidotoxicity, mediated by acid-sensing ion channels (ASICs), also contributes toward exacerbating the condition. A role for ASICs in stroke, through the activation of the inflammasome, is emerging, which opens new avenues for understanding and intervening in stroke. In this review, we describe the various types of inflammasomes and their mechanisms of activation in stroke. Furthermore, therapeutic approaches targeting the inflammasome and that may be helpful in improving stroke outcome are discussed.

show abstract