NMR determination of the composition of balsams from scotch pine resin

Скаковский, Е. Д.; Tychinskaya, L. Yu.; Gaidukevich, O. A.; Козлов, Н. Г.; Klyuev, A. Yu.; Lamotkin, S. A.; Shpak, S. I.; Рыков, С. В.

doi:10.1007/s10812-008-9065-y

Cited by 13 publications

(20 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…), and beeswax. Several of the signals originating from components in the resins appeared in the region of δ4.60–6.20 showing a chemical shift and coupling pattern which suggested the presence of levopimaric (LPA), abietic (AA), pimaric (PA), and isopimaric (IPA) acids [ 17 ] ( Figure 3 ). These abietane diterpenes are major components of the resins of conifers and have been isolated mainly from Pinaceae species [ 18 ].…”

Section: Resultsmentioning

confidence: 99%

An Insight to the Composition of Pre-Hispanic Mayan Funerary Pigments by 1H-NMR Analysis

et al. 2021

View full text Add to dashboard Cite

The funerary rites of particular members of the pre-Hispanic Mayan society included the pigmentation of the corpse with a red color. In order to understand this ritual, it is first necessary to identify the constituents of the pigment mixture and then, based on its properties, analyze the possible form and moment of application. In the present approach, 1H-NMR analysis was carried to detect organic components in the funerary pigments of Xcambó, a small Maya settlement in the Yucatan Peninsula. The comparison of the spectra belonging to the pigment found in the bone remains of seven individuals, and those from natural materials, led to the identification of beeswax and an abietane resin as constituents of the pigment, thus conferring it agglutinant and aromatic properties, respectively. The 1H-NMR analysis also allowed to rule out the presence of copal, a resin found in the pigment cover from paramount chiefs from the Mayan society. Additionally, a protocol for the extraction of the organic fraction from the bone segment without visible signs of analysis was developed, thus broadening the techniques available to investigate these valuable samples.

show abstract

Section: Resultsmentioning

confidence: 99%

An Insight to the Composition of Pre-Hispanic Mayan Funerary Pigments by 1H-NMR Analysis

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Another important lipid used in ancient ointment preparation is pine resin (Skakovskii et al . 2008).…”

Section: Resultsmentioning

confidence: 99%

¹H‐NMR AND³¹P‐NMR CHARACTERIZATION OF THE LIPID FRACTION IN ARCHAEOLOGICAL OINTMENTS

et al. 2012

View full text Add to dashboard Cite

Lipids are a broad group of naturally occurring molecules, which includes fats, oils, waxes, sterols, terpenes, fat-soluble vitamins, monoglycerides, diglycerides, phospholipids and others. Lipids have been widely used in human history and they are often present in archaeological finds. In particular, in the field of medicine and cosmetics, lipids have been employed as base in the preparation of unguents, salves and balms. The characterization of these products and their degradation products induced by ageing is important for an understanding of the ancient pharmaceutical techniques and to assess the state of conservation. 1 H-NMR and, for the first time, 31 P-NMR spectroscopy have been applied to the characterization of such compounds. By derivatization of the samples with 2-chloro-4,4,5,5-tetramethyl-1,3,2-dioxaphospholane, it is possible to recognize, quantify and evaluate the degradation degree of lipids in archaeological unguent samples. With this new approach, lipids have been recognized in 17th-century archaeological ointments from the Aboca Museum. Moreover, severe hydrolysis and oxidation markers have been detected.

show abstract

“…The identity of all compounds was defined by the literature data [41,73,74,75]. Due to rather complicated resin acids’ 1 H-NMR spectra, nonoverlapping, undoubtedly defined peaks of the olefinic or aromatic protons were selected for quantitation.…”

Section: Methodsmentioning

confidence: 99%

“…laricio [20,40]. Skakovskii et al [41], through the use of high-field NMR spectrometers introduced the alternative 1 H-NMR analysis of resin acids, and this technique is here preferred as quicker and straightforward. Moreover, the possible simultaneous record of 1 H-NMR and 13 C-NMR experiments can pave the way to the robust analysis of resin acid by combined data as performed for other systems [42].…”

Section: Introductionmentioning

confidence: 99%

High-Throughput 1H-Nuclear Magnetic Resonance-Based Screening for the Identification and Quantification of Heartwood Diterpenic Acids in Four Black Pine (Pinus nigra Arn.) Marginal Provenances in Greece

Ioannidis¹,

Melliou

Magiatis

2019

Molecules

View full text Add to dashboard Cite

A high-throughput quantitative Nuclear Magnetic Resonance 1H-NMR method was developed and applied to screen the quantity of the diterpenic resin acids in the heartwood of black pine, due to the renewed scientific interest in their medicinal properties and use in various diseases treatment. The 260 samples were taken from Pinus nigra clones, selected from four provenances of the Peloponnese (Greece), participating in a 35-year-old clonal seed orchard. Total resin acids per dry heartwood weight (dhw) varied greatly, ranging from 30.05 to 424.70 mg/gdhw (average 219.98 mg/gdhw). Abietic was the predominant acid (76.77 mg/gdhw), followed by palustric acid (47.94 mg/gdhw), neoabietic acid (39.34 mg/gdhw), and pimaric acid (22.54 mg/gdhw). Dehydroabietic acid was at moderate levels (11.69 mg/gdhw), while levopimaric, isopimaric, and sandaracopimaric acids were in lower concentrations. The resin acid fraction accounted for 72.33% of the total acetone extractives. Stilbenes were presented in significant quantities (19.70%). The resin acid content was composed mainly of the abietane type resin acids (83.56%). Peloponnesian Pinus nigra heartwood was found to be the richest source of resin acids identified to date and is considered the best natural source for the production of such bioactive extracts. The results indicate a high potential for effective selection and advanced breeding of pharmaceutical and high economic value bioactive substances from Pinus nigra clones.

show abstract

NMR determination of the composition of balsams from scotch pine resin

Cited by 13 publications

References 6 publications

An Insight to the Composition of Pre-Hispanic Mayan Funerary Pigments by 1H-NMR Analysis

An Insight to the Composition of Pre-Hispanic Mayan Funerary Pigments by 1H-NMR Analysis

¹H‐NMR AND³¹P‐NMR CHARACTERIZATION OF THE LIPID FRACTION IN ARCHAEOLOGICAL OINTMENTS

High-Throughput 1H-Nuclear Magnetic Resonance-Based Screening for the Identification and Quantification of Heartwood Diterpenic Acids in Four Black Pine (Pinus nigra Arn.) Marginal Provenances in Greece

Contact Info

Product

Resources

About

NMR determination of the composition of balsams from scotch pine resin

Cited by 13 publications

References 6 publications

An Insight to the Composition of Pre-Hispanic Mayan Funerary Pigments by 1H-NMR Analysis

An Insight to the Composition of Pre-Hispanic Mayan Funerary Pigments by 1H-NMR Analysis

1H‐NMR AND31P‐NMR CHARACTERIZATION OF THE LIPID FRACTION IN ARCHAEOLOGICAL OINTMENTS

High-Throughput 1H-Nuclear Magnetic Resonance-Based Screening for the Identification and Quantification of Heartwood Diterpenic Acids in Four Black Pine (Pinus nigra Arn.) Marginal Provenances in Greece

Contact Info

Product

Resources

About

¹H‐NMR AND³¹P‐NMR CHARACTERIZATION OF THE LIPID FRACTION IN ARCHAEOLOGICAL OINTMENTS