NMR evidence of specific base-pairing between purines and pyrimidines

Shoup, Regitze R.; Miles, H. Todd; Becker, Edwin D.

doi:10.1016/0006-291x(66)90527-4

Cited by 160 publications

(30 citation statements)

References 14 publications

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“…Several studies were carried out using bases substituted at the glycosyl nitrogen and solvents such as tetrachloromethane, chloroform, and dimethylsulfoxide. Base-base association has been monitored as change in amino group signals by both NMR and IR methods ( Figure 6-5) (472,473), and has been studied by calorimetric and osmometric methods.…”

Section: The Stability and Formation Of Base Pairs As Determined By Tmentioning

confidence: 99%

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Principles of Nucleic Acid Structure

Saenger

1984

Springer Advanced Texts in Chemistry

5,985

312

6,279

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Section: The Stability and Formation Of Base Pairs As Determined By Tmentioning

confidence: 99%

“…Recorded with a Varian A-60 spectrometer; abscissa given in cps (cycles per second) as downfield shifts from internal TMS (tetramethylsilane) standard. From(472). (Bottom) Infrared absorption spectra of 2,6-diamino-9-ethylpurine (A), l-cyclohexyl-5-bromouracil (B), and their 1: 1 mixture (C), all at 0.002 Min deuterochloroform.…”

mentioning

confidence: 99%

Principles of Nucleic Acid Structure

Saenger

1984

Springer Advanced Texts in Chemistry

5,985

312

6,279

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“…The evidence for specificity of base pairing between urazole and adenine was sought by following the IH-NMR method of Shoup et al (1966). Hydrogen bonding was measured as a downfield shift of the N-H protons of 9-ethyladenine, 1-methyluracil, and 1-methylurazole.…”

Section: Base Pairing Between Urazole and Adeninementioning

confidence: 99%

Alternative bases in the RNA world: The prebiotic synthesis of urazole and its ribosides

1994

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We have prepared a [32P]-labled oligonucleotide probe carrying a free primary amine at its 3'-terminus. This probe is used to initiate polymerization of aziridine (ethyleneimine) in aqueous solution. The nature of the oligomeric products and the kinetics of their formation are then monitored by gel electrophoresis. Our results are generally consistent with those obtained using conventional techniques. We have also investigated the effect of polyanionic templates on the rate of oligomerization of aziridine. We find that water-soluble polyanions generally accelerate the polymerization. The sodium salt of polymethacrylic acid is the most effective of the templates that we studied. The methods introduced in this paper should be applicable to a variety of polymerization reactions in aqueous solution. They should greatly simplify the screening of potentially prebiotic polymerization reactions.

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“…• В самой ранней работе по изучению алкилпроизводных оснований [36 ] было показано, что в ДМСО наиболее легко образуется уотсон-криковская пара G-C. Образование пары А-Т существенно менее выгодно, однако следы ее образования были все же зарегистрированы.…”

Section: Au" = N{w Ss (Bp) -U"\-l(ujm) -U Ss ) --(!/"(«)у ; Au Sb = Nunclassified