NMR spectral analysis of a water-insoluble (1 → 3)-β-d-glucan isolated from Saccharomyces cerevisiae

He, Ensley; Tobias, Brian; Ha, Pretus; Rb, McNamee; El, Jones; Iw, Browder; Williams, Dennis

doi:10.1016/0008-6215(94)84098-9

Cited by 78 publications

(42 citation statements)

References 12 publications

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“…1. The major signals in the spectra corresponded to those of 1,3-linked b-D-glucan and were assigned by comparison with data reported by Ensley et al (1994). The coupling constant of the anomeric doublet at 4.52 ppm ( 3 J H-1,H-2 = 7.6 Hz) supported the interpretation that the 3-linked D-Glcp had the b-configuration rather than the a-configuration, for which the corresponding coupling constant is generally less than 5 Hz (Teng et al, 2002).…”

Section: Carbohydrate Analysismentioning

confidence: 74%

The glucans extracted with warm water from diatoms are mainly derived from intracellular chrysolaminaran and not extracellular polysaccharides

Chiovitti

Molino

Crawford

et al. 2004

European Journal of Phycology

106

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Several recent studies have employed warm-water treatment of diatom cells to extract nominally bound extracellular polymeric substances. Where examined, the dominant neutral sugar in these extracts was glucose. In the present study, we sought to characterize the structure of the glucose-rich polymers in the water extracts of diatoms and to determine the origin of these polymers. The marine diatoms surveyed were Phaeodactylum tricornutum, Cylindrotheca fusiformis, Craspedostauros australis and Thalassiosira pseudonana. A freshwater species, Pinnularia viridis, was also investigated for the dye labelling experiments. Freshly harvested marine diatoms were extracted with water at 308C for 1 h. Constituent monosaccharide analyses showed that glucose was the dominant neutral sugar (80 -95 mol% of the total) in the extracts from three marine species, whereas the P. tricornutum extract contained predominantly ribose, galactose and glucose, and was inferred to be enriched in low-molecular-weight components. Linkage analysis of the constituent monosaccharides and proton nuclear magnetic resonance spectroscopy showed that the glucose in these extracts was derived primarily from 1,3-b-D-glucan. Immunocytochemistry with a monoclonal anti-1,3-b-D-glucan antibody confirmed that the glucan was localized in the vacuoles of diatom cells preserved by freeze-substitution. Nearly all diatom cells incubated with a fluorescent dye, DiBAC 4 (3), during warm water treatment at 308C or 608C incorporated the dye, demonstrating that the membrane integrity of the diatoms was compromised and supporting the contention that intracellular glucan was released during the treatment. In light of these data, the extracellular glucans of diatoms reported in some previous studies are re-interpreted as intracellular chrysolaminaran.

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Section: Carbohydrate Analysismentioning

confidence: 74%

The glucans extracted with warm water from diatoms are mainly derived from intracellular chrysolaminaran and not extracellular polysaccharides

Chiovitti

Molino

Crawford

et al. 2004

European Journal of Phycology

106

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“…Fluorescein isothiocyanate (FITC)-labeled zymosan particles and Alexa Fluor 488 carboxylic acid, succinimidyl ester were obtained from Invitrogen. Particulate b-glucan was purified from Saccharomyces cerevisiae and structurally characterized by NMR (19). Endotoxin-free water-soluble glucan phosphate (S-GP) was prepared from particulate b-glucan as previously described (20).…”

Section: Methodsmentioning

confidence: 99%

Cytosolic Phospholipase A₂ Activation by Candida albicans in Alveolar Macrophages

Parti

Loper²,

Brown³

et al. 2010

Am J Respir Cell Mol Biol

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“…Water-insoluble (133)-␤-D-glucan was extracted from Saccharomyces cerevisiae (49). Insoluble glucan was converted to a watersoluble form as described by Williams et al (49) and chemically characterized as previously described (11,19,24,25,49). The final product was stored (Ϫ80°C) as a lyophilized powder.…”

Section: Methodsmentioning

confidence: 99%

Normal Human Fibroblasts Express Pattern Recognition Receptors for Fungal (1→3)-β-d-Glucans

Kougias¹,

Wei²,

Rice³

et al. 2001

Infect Immun

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NMR spectral analysis of a water-insoluble (1 → 3)-β-d-glucan isolated from Saccharomyces cerevisiae

Cited by 78 publications

References 12 publications

The glucans extracted with warm water from diatoms are mainly derived from intracellular chrysolaminaran and not extracellular polysaccharides

The glucans extracted with warm water from diatoms are mainly derived from intracellular chrysolaminaran and not extracellular polysaccharides

Cytosolic Phospholipase A₂ Activation by Candida albicans in Alveolar Macrophages

Normal Human Fibroblasts Express Pattern Recognition Receptors for Fungal (1→3)-β-d-Glucans

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NMR spectral analysis of a water-insoluble (1 → 3)-β-d-glucan isolated from Saccharomyces cerevisiae

Cited by 78 publications

References 12 publications

The glucans extracted with warm water from diatoms are mainly derived from intracellular chrysolaminaran and not extracellular polysaccharides

The glucans extracted with warm water from diatoms are mainly derived from intracellular chrysolaminaran and not extracellular polysaccharides

Cytosolic Phospholipase A2 Activation by Candida albicans in Alveolar Macrophages

Normal Human Fibroblasts Express Pattern Recognition Receptors for Fungal (1→3)-β-d-Glucans

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Cytosolic Phospholipase A₂ Activation by Candida albicans in Alveolar Macrophages