NMR spectroscopic structural characterization of a water-soluble β-(1 → 3, 1 → 6)-glucan from Aureobasidium pullulans

Kono, Hiroyuki; Kondo, Nobuhiro; Hirabayashi, Katsuki; Ogata, Makoto; Totani, Kiichiro; Ikematsu, Shinya; Osada, Motonobu

doi:10.1016/j.carbpol.2017.07.018

Cited by 55 publications

(30 citation statements)

References 38 publications

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“…The 13 C NMR analyses of fractions GW-s ( Figure 3b) and LW-s (data not shown) showed signals of (1→3)-linked β-Glcp residues at 102.4, 72.2, 85.6, 67.9, 75.8, and 60.4 ppm, which were attributed to C-1-C-6, respectively. Additionally, the HSQC spectrum ( Figure 4b (Figure 4), COSY ( Figure S1), and TOCSY ( Figure S2) spectra, presented in Table 4 are in agreement with previously reported resonances [38][39][40][41]. In accordance with the methylation analysis, the NMR data suggest the presence of a highly branched β- NMR spectrum (Figure 5c) showed signals corresponding to the carbonyl carbon of the main chain and carboxylic groups of the acidic amino acids side chain (180-165 ppm), aromatic amino acids (140-115 ppm),  and  carbons (65-45 ppm and 45-25 ppm, respectively), and methylene and methyl groups of the aliphatic side chains (2510 ppm) [47].…”

Section: Extraction and Characterization Of Polysaccharides From P Gsupporting

confidence: 91%

Marine Microalgae Biomolecules and Their Adhesion Capacity to Salmonella enterica sv. Typhimurium

et al. 2020

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Different molecules have been tested as analog receptors due to their capacity to bind bacteria and prevent cell adhesion. By using in vitro assays, the present study characterized the aqueous and alkaline extracts from microalgae Pavlova lutheri and Pavlova gyrans and evaluated the capacity of these extracts to adhere to enterobacteria (Salmonella Typhimurium). The aqueous and alkaline extracts of both species were fractionated via freeze-thawing, giving rise to soluble and insoluble (precipitate) fractions in cold water. The obtained fractions were studied using thermogravimetric, methylation analyses, and using 1D and 2D NMR techniques. The cold-water-soluble fractions obtained from the aqueous extracts were mainly composed of highly branched (1→3),(1→6)-β-glucans, whereas the cold-water-precipitate fractions were constituted by (1→3)-β-glucans. The alkaline extract fractions showed similar compositions with a high protein content, and the presence of glycosides (sulfoquinovosylglycerol (SQG), digalactosylglycerol (DGG)), and free fatty acids. The linear (1→3)-β-glucans and the alkaline extract fractions showed an adhesion capacity toward Salmonella. The chemical composition of the active fractions suggested that the presence of three-linked β-glucose units, as well as microalgal proteins and glycosides, could be important in the adhesion process. Therefore, these microalgal species possess a high potential to serve as a source of anti-adhesive compounds.

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Section: Extraction and Characterization Of Polysaccharides From P Gsupporting

confidence: 91%

Marine Microalgae Biomolecules and Their Adhesion Capacity to Salmonella enterica sv. Typhimurium

et al. 2020

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“…Aureobasidium pullulans was also isolated in our study; it has previously been reported to synthesize biomolecules of medicinal importance, for example, β-(1 → 3, 1 → 6)-glucans, which displays anti-tumour, immune-modulatory and food allergy inhibition [39], fungicide [40] and antimicrobial activity [41]. It also shows fungicide and fungicidal properties against Candida albicans [42].…”

Section: Discussionmentioning

confidence: 84%

Diversity and Antimicrobial Activity of Culturable Fungal Endophytes in Solanum mauritianum

Pelo

Mavumengwana

Green

2020

IJERPH

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Plant endophytes are microbial sources of bioactive secondary metabolites, which mimic the natural compounds chemistry of their respective host plants in a similar manner. This study explored the isolation and identification of fungal endophytes, and investigated the antibacterial and antimycobacterial activity of their crude extracts. Fungal endophytes were isolated from Solanum mauritianum, identified using morphological traits and internal transcribed spacer ribosomal-deoxyribonucleic acid (ITS-rDNA) sequence analysis. Eight fungal endophytes were identified as Aureobasidium pullulans, Paracamarosporium leucadendri, Cladosporium sp., Collectotrichum boninense, Fusarium sp., Hyalodendriella sp., and Talaromyces sp., while Penicillium chrysogenum was isolated from the leaves and unripe fruits. Good activity was observed for the crude extracts of Paracamarosporium leucadendri inhibiting Mycobacterium bovis, Klebsiella pneumoniae, and Pseudomonas aeruginosa at 6 µg/mL. Crude extracts of Fusarium sp., showed activity at 9 μg/mL against M. bovis, M. smegmatis and K. pneumonia. In general, the crude extracts showed great activity against Gram-negative and Gram-positive bacteria and novel results for two mycobacteria species M. bovis and M. smegmatis. The results provide evidence of diverse fungal endophytes isolated from Solanum mauritianum, and evidence that fungal endophytes are a good source of bioactive compounds with pharmaceutical potential, particularly against Mycobacterium tuberculosis.

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“…Various methods for the quantitative determination of β-glucans are described in the literature. Among them, the following can be distinguished: the immunoenzymatic method (ELISA) [ 51 ], the fluorimetric method using aniline blue [ 29 , 52 ], the spectrophotometric method with enzymatic hydrolysis [ 53 , 54 , 55 ] or the spectrophotometric method using Congo red [ 29 ], high-performance liquid chromatography (HPLC) [ 56 ] and others like Nuclear Magnetic Resonance (NMR) [ 57 ].…”

Section: Resultsmentioning

confidence: 99%

“…The NMR spectroscopy is useful for the investigation of glucan conformation and gives information on the anomeric configuration and ring conformation, position, and proportion of branching. This method is accurate, but data interpretation is complicated [ 8 , 57 ].…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Polish Wild Mushrooms as Beta-Glucan Sources

Mirończuk-Chodakowska

Witkowska

2020

IJERPH

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Mushroom beta-glucans show immunomodulatory, anticancer and antioxidant features. Numerous papers have been published in the last years on fungal polysaccharides, especially beta-glucans, demonstrating their various biological activities. However substantial data about beta-glucan contents in many mushroom species, especially wild mushrooms, are still missing. Therefore, the main objective of the study was to evaluate β-glucans in 18 species of wild mushrooms and three species of commercial mushrooms for comparison purposes. The contents of β-glucans were determined by the Megazyme method and with the Congo red method, which differ in analytical procedure. Among wild mushrooms, the highest mean β-glucan content assessed with the Megazyme method was found in Tricholoma portentosum (34.97 g/100 g DM), whereas with the Congo red method in Lactarius deliciosus (17.11 g/100 g DM) and Suillus grevillei (16.97 g/100 g DM). The β-glucans in wild mushrooms assessed with the Megazyme method were comparable to commercial mushrooms, whereas β-glucans assessed with the Congo red method were generally higher in wild mushrooms, especially in Russula vinosa, L. deliciosus and S. grevillei. This study indicates wild mushrooms as interesting material for β-glucan extraction for food industry and medicinal purposes.

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NMR spectroscopic structural characterization of a water-soluble β-(1 → 3, 1 → 6)-glucan from Aureobasidium pullulans

Cited by 55 publications

References 38 publications

Marine Microalgae Biomolecules and Their Adhesion Capacity to Salmonella enterica sv. Typhimurium

Marine Microalgae Biomolecules and Their Adhesion Capacity to Salmonella enterica sv. Typhimurium

Diversity and Antimicrobial Activity of Culturable Fungal Endophytes in Solanum mauritianum

Evaluation of Polish Wild Mushrooms as Beta-Glucan Sources

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