NMR studies on Na+ transport in Synechococcus PCC 6311

Nitschmann, Wolfgang H.; Packer, Lester

doi:10.1016/0003-9861(92)90694-r

Cited by 28 publications

(21 citation statements)

References 21 publications

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“…As a first line of defense, the activation of Na 1 -export mechanisms such as Na 1 /H 1 -antiporters and Na 1 /ATPases results in the reduction of the sodium concentration to control levels within minutes (Nitschmann and Packer, 1992). By flame photometry, we could not follow the fast influx and efflux of sodium ions.…”

Section: Salt Stress Response Includes Activation Of Ion Transport Anmentioning

confidence: 73%

Gene Expression Profiling Reflects Physiological Processes in Salt Acclimation of Synechocystis sp. Strain PCC 6803

Marin

Kanesaki²,

Los³

et al. 2004

Plant Physiology

114

199

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The kinetics of genome-wide responses of gene expression during the acclimation of cells of Synechocystis sp. PCC 6803 to salt stress were followed by DNA-microarray technique and compared to changes in main physiological parameters. During the first 30 min of salt stress, about 240 genes became induced higher than 3-fold, while about 140 genes were repressed. However, most changes in gene expression were only transient and observed among genes for hypothetical proteins. At 24 h after onset of salt stress conditions, the expression of only 39 genes remained significantly enhanced. Among them, many genes that encode proteins essential for salt acclimation were detected, while only a small number of genes for hypothetical proteins remained activated. Following the expression of genes for main functions of the cyanobacterial cell, i.e. PSI, PSII, phycobilisomes, and synthesis of compatible solutes, such as ion homeostasis, distinct kinetic patterns were found. While most of the genes for basal physiological functions were transiently repressed during the 1st h after the onset of salt stress, genes for proteins specifically related to salt acclimation were activated. This gene expression pattern reflects well the changes in main physiological processes in salt-stressed cells, i.e. transient inhibition of photosynthesis and pigment synthesis as well as immediate activation of synthesis of compatible solutes. The results clearly document that following the kinetics of genome-wide expression, profiling can be used to envisage physiological changes in the cyanobacterial cell after certain changes in growth conditions.

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Section: Salt Stress Response Includes Activation Of Ion Transport Anmentioning

confidence: 73%

Gene Expression Profiling Reflects Physiological Processes in Salt Acclimation of Synechocystis sp. Strain PCC 6803

Marin

Kanesaki²,

Los³

et al. 2004

Plant Physiology

114

199

View full text Add to dashboard Cite

show abstract

“…Na + ) transporters within minutes, while other ions (e.g. K + ) may be accumulated intracellularly (Nitschmann and Packer 1992). Changes in the composition of cytosolic ions generally decrease the stability or activity of many cellular proteins, but other enzymatic activities are directly activated by ions such as Na+ in a process that is independent of transcriptional changes (Marin et al 2004; Kanesaki et al 2002; Schoor et al 1999).…”

Section: Cyanobacterial Osmotic Stress Response Pathwaysmentioning

confidence: 99%

Engineering cyanobacteria as photosynthetic feedstock factories

2014

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Carbohydrate feedstocks are at the root of bioindustrial production and are needed in greater quantities than ever due to increased prioritization of renewable fuels and reduction of carbon emissions. Cyanobacteria possess a number of features that make them well-suited as an alternative feedstock crop in comparison to traditional, terrestrial plant species. Recent advances in genetic engineering, as well as promising preliminary investigations of cyanobacteria in a number of distinct production regimes have illustrated the potential of these aquatic phototrophs as biosynthetic chasses. Further improvements in strain productivities and design, along with enhanced understanding of photosynthetic metabolism in cyanobacteria may pave the way to translate cyanobacterial theoretical potential into realized application.

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“…Transcriptional profile of nha genes. Na ϩ /H ϩ antiporters are considered proteins that are involved in the first line of defense under salt stress conditions (36). Thus, we examined whether the expression of the nha1 to nha7 genes is subject to Na ϩ -and pH-dependent regulation at the transcriptional level with respect to acute response and acclimation.…”

mentioning

confidence: 99%

Two Members of a Network of Putative Na ⁺ /H ⁺ Antiporters Are Involved in Salt and pH Tolerance of the Freshwater Cyanobacterium Synechococcus elongatus

2008

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Synechococcus elongatus strain PCC 7942 is an alkaliphilic cyanobacterium that tolerates a relatively high salt concentration as a freshwater microorganism. Its genome sequence revealed seven genes, nha1 to nha7 (syn_pcc79420811, syn_pcc79421264, syn_pcc7942359, syn_pcc79420546, syn_pcc79420307, syn_pcc79422394, and syn_pcc79422186), and the deduced amino acid sequences encoded by these genes are similar to those of Na ؉ /H ؉ antiporters. The present work focused on molecular and functional characterization of these nha genes encoding Na ؉ /H ؉ antiporters. Our results show that of the nha genes expressed in Escherichia coli, only nha3 complemented the deficient Na ؉ /H ؉ antiporter activity of the Na ؉ -sensitive TO114 recipient strain. Moreover, two of the cyanobacterial strains with separate disruptions in the nha genes (⌬nha1, ⌬nha2, ⌬nha3, ⌬nha4, ⌬nha5, and ⌬nha7) had a phenotype different from that of the wild type. In particular, ⌬nhA3 cells showed a high-salt-and alkaline-pH-sensitive phenotype, while ⌬nha2 cells showed low salt and alkaline pH sensitivity. Finally, the transcriptional profile of the nha1 to nha7 genes, monitored using the real-time PCR technique, revealed that the nha6 gene is upregulated and the nha1 gene is downregulated under certain environmental conditions.

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NMR studies on Na+ transport in Synechococcus PCC 6311

Cited by 28 publications

References 21 publications

Gene Expression Profiling Reflects Physiological Processes in Salt Acclimation of Synechocystis sp. Strain PCC 6803

Gene Expression Profiling Reflects Physiological Processes in Salt Acclimation of Synechocystis sp. Strain PCC 6803

Engineering cyanobacteria as photosynthetic feedstock factories

Two Members of a Network of Putative Na ⁺ /H ⁺ Antiporters Are Involved in Salt and pH Tolerance of the Freshwater Cyanobacterium Synechococcus elongatus

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NMR studies on Na+ transport in Synechococcus PCC 6311

Cited by 28 publications

References 21 publications

Gene Expression Profiling Reflects Physiological Processes in Salt Acclimation of Synechocystis sp. Strain PCC 6803

Gene Expression Profiling Reflects Physiological Processes in Salt Acclimation of Synechocystis sp. Strain PCC 6803

Engineering cyanobacteria as photosynthetic feedstock factories

Two Members of a Network of Putative Na + /H + Antiporters Are Involved in Salt and pH Tolerance of the Freshwater Cyanobacterium Synechococcus elongatus

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Two Members of a Network of Putative Na ⁺ /H ⁺ Antiporters Are Involved in Salt and pH Tolerance of the Freshwater Cyanobacterium Synechococcus elongatus