No difference in antibody titers against xenotropic MLV related virus in prostate cancer cases and cancer-free controls

Sabunciyan, Sarven; Mandelberg, Nataniel; Rabkin, Charles S.; Yolken, Robert H.; Viscidi, Raphael P.

doi:10.1016/j.mcp.2011.01.005

Cited by 10 publications

(9 citation statements)

References 7 publications

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“…Xenotropic murine leukemia virus-related virus (XMRV) is a gammaretrovirus that was first identified in some prostate cancer tissues ( 1 , 2 ) While some subsequent reports confirmed the presence of XMRV in prostate cancer samples ( 3–6 ), several others found little or no evidence of the virus in patient samples ( 7–9 ). XMRV DNA was also reported in 67% of patients with chronic fatigue syndrome (CFS) ( 10 ), but several subsequent studies in Europe and the USA failed to identify XMRV DNA in CFS patients or healthy controls ( 11–15 ).…”

Section: Introductionmentioning

confidence: 99%

Biochemical, inhibition and inhibitor resistance studies of xenotropic murine leukemia virus-related virus reverse transcriptase

Ndongwe

Adedeji

Michailidis

et al. 2011

Nucleic Acids Research

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We report key mechanistic differences between the reverse transcriptases (RT) of human immunodeficiency virus type-1 (HIV-1) and of xenotropic murine leukemia virus-related virus (XMRV), a gammaretrovirus that can infect human cells. Steady and pre-steady state kinetics demonstrated that XMRV RT is significantly less efficient in DNA synthesis and in unblocking chain-terminated primers. Surface plasmon resonance experiments showed that the gammaretroviral enzyme has a remarkably higher dissociation rate (koff) from DNA, which also results in lower processivity than HIV-1 RT. Transient kinetics of mismatch incorporation revealed that XMRV RT has higher fidelity than HIV-1 RT. We identified RNA aptamers that potently inhibit XMRV, but not HIV-1 RT. XMRV RT is highly susceptible to some nucleoside RT inhibitors, including Translocation Deficient RT inhibitors, but not to non-nucleoside RT inhibitors. We demonstrated that XMRV RT mutants K103R and Q190M, which are equivalent to HIV-1 mutants that are resistant to tenofovir (K65R) and AZT (Q151M), are also resistant to the respective drugs, suggesting that XMRV can acquire resistance to these compounds through the decreased incorporation mechanism reported in HIV-1.

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Section: Introductionmentioning

confidence: 99%

Biochemical, inhibition and inhibitor resistance studies of xenotropic murine leukemia virus-related virus reverse transcriptase

Ndongwe

Adedeji

Michailidis

et al. 2011

Nucleic Acids Research

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“…Another study indicated that as high as 7% of the US blood donors were positive for MLV line sequences 20 . However, several studies failed to detect XMRV in the general populations in the United States 7,8,13,14,33 . Qiu and coworkers 22 reported that 0.1% (3/2854) of the US blood donors were anti‐XMRV positive by using their prototype immunoassay.…”

Section: Discussionmentioning

confidence: 99%

“…The finding was further confirmed by another four studies, 2‐5 although several studies failed to detect XMRV in prostate cancer patients. For example, three studies from North America and Mexico did not detect XMRV in patients with prostate cancer 6‐8 . Two studies from Europe could not detect XMRV in prostate cancer tissues 9,10 .…”

mentioning

confidence: 99%

Absence of xenotropic murine leukemia virus–related virus in blood donors in China

Zhang

et al. 2011

Transfusion

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“…Several groups were unable to detect XMRV-specific antibodies in PC and CFS patients as well as healthy control sera [30, 35, 36, 38]. In some studies, the weak antibody activity could not be confirmed by other assays, such as immunofluorescence assay (IFA) or was found to be nonspecific cross-reactivity [38].…”

Section: Xmrv Testing Methodsmentioning

confidence: 99%

Testing Strategies for Detection of Xenotropic Murine Leukemia Virus-Related Virus Infection

Tang

Hewlett

2011

Advances in Virology

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Xenotropic murine leukemia virus-related virus (XMRV) is a newly identified gamma retrovirus and may be associated with prostate cancer- (PC) and chronic fatigue syndrome (CFS). Since its identification in 2006 and detection of polytropic murine lenkemia virus (MLV)-like sequences in CFS patients in 2010, several test methods including nucleic acid testing methods and serological assays have been developed for detection of XMRV and/or MLV-like sequences. However, these research assays have not yet been validated and evaluated due to the lack of well-characterized reference materials. Mouse DNA contamination should be carefully checked when testing human specimens in order to avoid false-positive detection of XMRV or MLV-like sequences.

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No difference in antibody titers against xenotropic MLV related virus in prostate cancer cases and cancer-free controls

Cited by 10 publications

References 7 publications

Biochemical, inhibition and inhibitor resistance studies of xenotropic murine leukemia virus-related virus reverse transcriptase

Biochemical, inhibition and inhibitor resistance studies of xenotropic murine leukemia virus-related virus reverse transcriptase

Absence of xenotropic murine leukemia virus–related virus in blood donors in China

Testing Strategies for Detection of Xenotropic Murine Leukemia Virus-Related Virus Infection

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