No restriction of intrathyroidal T cell receptor Vα families in the thyroid of Graves' disease

McIntosh, Richard S.; Watson, P.; Pickerill, A P; Davies, Richard J.; Weetman, Anthony P.

doi:10.1111/j.1365-2249.1993.tb03370.x

Cited by 32 publications

(5 citation statements)

References 36 publications

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“…Restricted usage of TCR Va as well as TCR Vb was also shown in T cells infiltrating extrathyroidal sites in addition to thyroid from Graves' disease [17]. On the contrary, other reports demonstrated no restriction of intrathyroidal TCR Va subfamilies in Graves' disease [18][19][20]. In Hashimoto's thyroiditis, a marked restriction of Va usage in intrathyroidal CD8 + T cell but not in CD4 + T cell has been shown in a small-scale study [7].…”

Section: Discussionmentioning

confidence: 93%

Analysis of T cell receptor Vβ diversity in peripheral CD4+ and CD8+ T lymphocytes in patients with autoimmune thyroid diseases

Okajima

Wada

Nishida

et al. 2008

Clinical and Experimental Immunology

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Summary Autoimmune thyroid diseases are characterized by intrathyroidal infiltration of CD4+ and CD8 + T lymphocytes reactive to self-thyroid antigens. Early studies analysing T cell receptor (TCR) Va gene usage have shown oligoclonal expansion of intrathyroidal T lymphocytes but not peripheral blood T cells. However, TCR Vb diversity of the isolated CD4 + and CD8 + T cell compartments in the peripheral blood has not been characterized fully in these patients. We performed complementarity-determining region 3 (CDR3) spectratyping as well as flow cytometric analysis for the TCR Vb repertoire in peripheral CD4+ and CD8 + T cells from 13 patients with Graves' disease and 17 patients with Hashimoto's thyroiditis. Polyclonal TCR Vb repertoire was demonstrated by flow cytometry in both diseases. In contrast, CDR3 spectratyping showed significantly higher skewing of TCR Vb in peripheral CD8 + T cells but not CD4+ T cells among patients with Hashimoto's thyroiditis compared with healthy adults. We found trends towards a more skewed CDR3 size distribution in those patients having disease longer than 5 years and requiring thyroid hormone replacement. Patients with Graves' disease exhibited no skewing both in CD4 + and CD8 + T cells. These findings indicate that clonal expansion of CD8 + T cells in Hashimoto's thyroiditis can be detected in peripheral blood and may support the role of CD8+ T cells in cell-mediated autoimmune attacks on the thyroid gland in Hashimoto's thyroiditis.

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Section: Discussionmentioning

confidence: 93%

Analysis of T cell receptor Vβ diversity in peripheral CD4+ and CD8+ T lymphocytes in patients with autoimmune thyroid diseases

Okajima

Wada

Nishida

et al. 2008

Clinical and Experimental Immunology

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“…As illustrated by the report in last month's issue of this journal by Mclntosh et al [28], this is also the case in autoimmune thyroid disease (also see Table 1). …”

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confidence: 81%

In search of TCR restriction in autoreactive T cell in human autoimmunology why is it so elusive?

Navarrete

Bottazzo

1993

Clinical and Experimental Immunology

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“…MA). Membranes were hybridized as described previously [26] with an internal Cii oligonucleotide (C<S3 of [10]), Densitoinetry of hybridized bands was carried out using the Bio-Proltle Image Analysis System (Version 4,01. Vilbcr-Lourmat, France).…”

Section: Tcr Kfi Atuilyvismentioning

confidence: 99%

The γδ T cell repertoire in Graves' disease and multinodular goitre

McIntosh

Tandon

Pickerill

et al. 1993

Clinical and Experimental Immunology

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SUMMARY γδ T cells are a subset of T cells with unknown function, and restriction of the γδ T cell receptor (TCR) repertoire has been described in rheumatoid arthritis and multiple sclerosis. Elevated numbers of γδ cells have been reported in the peripheral blood and thyroids of patients with Graves' disease. We have carried out flow cytometric analysis on peripheral blood mononuclear cells (PBMC) and intrathyroidal lymphocytes (ITL) from 12 patients with Graves' disease and nine patients with multinodular goitre (MNG). a thyroid disease of unknown etiology. There was no significant difference between the proportion of γδ T cells in the PBMC of Graves' and MNG patients, nor between the PBMC and ITL populations in either patient group. We have also carried out polymerase chain reaction amplification on RNA prepared from matched PBMC. ITL and the activated (CD25+) subset of ITL using six TCR γδ‐family specific primers. Although there were differences in the amounts of each γδ transcript amplified from PBMC and ITL, there was no difference between the two patient groups. No consistent differences were therefore found between the γδ T cell populations in Graves' and MNG patients, arguing against the direct involvement of this T cell subset in the pathogenesis of Graves' disease.

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No restriction of intrathyroidal T cell receptor Vα families in the thyroid of Graves' disease

Cited by 32 publications

References 36 publications

Analysis of T cell receptor Vβ diversity in peripheral CD4+ and CD8+ T lymphocytes in patients with autoimmune thyroid diseases

Analysis of T cell receptor Vβ diversity in peripheral CD4+ and CD8+ T lymphocytes in patients with autoimmune thyroid diseases

In search of TCR restriction in autoreactive T cell in human autoimmunology why is it so elusive?

The γδ T cell repertoire in Graves' disease and multinodular goitre

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