Nociception in chicken embryos, Part I: Analysis of cardiovascular responses to a mechanical noxious stimulus

Weiss, Larissa; Saller, Anna M.; Werner, Julia; Suess, Stephanie C.; Reiser, Judith; Kollmansperger, Sandra; Anders, Malte; Fenzl, Thomas; Schusser, Benjamin; Baumgartner, Christine

doi:10.1101/2023.04.14.536899

Cited by 6 publications

(2 citation statements)

References 52 publications

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“…During Post Pinch, the observed movement changes in ED15 to ED18 embryos were most likely a response to the noxious mechanical stimulus and can therefore be interpreted as nocifensive behavior. The results of our current movement analysis in combination with the corresponding results of the cardiovascular changes 22 and the evaluation of the onset of physiological neuronal signals 23 in chicken embryos during this developmental period provide valuable information that enhances our understanding of the development of nociception and pain perception in chicken.…”

Section: Discussionmentioning

confidence: 99%

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Nociception in chicken embryos, Part III: Analysis of movements before and after application of a noxious stimulus

Suess

Werner

Saller

et al. 2023

Preprint

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Many potentially noxious interventions are performed on chicken embryos in research and in the poultry industry. It is therefore essential and in the interest of animal welfare to be able to precisely define the point at which a chicken embryo is capable of nociception in ovo. The present part III of a comprehensive study examined the movements of developing chicken embryos with the aim of identifying behavioral responses to a noxious stimulus. For this purpose, a noxious mechanical stimulus and a control stimulus were applied in a randomized order. The recorded movements of the embryos were evaluated using the markerless pose estimation software DeepLabCut and manual observations. After the application of the mechanical stimulus, a significant increase in beak movement was identified in 15- to 18-day-old embryos. In younger embryos, no behavioral changes related to the noxious stimulus were observed. The presented results results indicate that noxious mechanical stimuli at the beak base evoke a nocifensive reaction in chicken embryos starting at embryonic day 15.

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Section: Discussionmentioning

confidence: 99%

“…The markerless pose estimation software DeepLabCut (DLC) and manual observations were used to analyze embryonic behavior [19][20][21] . In addition, cardiovascular 22 and electrophysiological 23 parameters were investigated in parts I and II of the comprehensive study.…”

Section: Introductionmentioning

confidence: 99%

Nociception in chicken embryos, Part III: Analysis of movements before and after application of a noxious stimulus

Suess

Werner

Saller

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

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Nociception in Chicken Embryos, Part III: Analysis of Movements before and after Application of a Noxious Stimulus

Süß,

Werner,

Saller

et al. 2023

Animals

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Many potentially noxious interventions are performed on chicken embryos in research and in the poultry industry. It is therefore essential and in the interest of animal welfare to be able to precisely define the point at which a chicken embryo is capable of nociception in ovo. The present part III of a comprehensive study examined the movements of developing chicken embryos with the aim of identifying behavioral responses to a noxious stimulus. For this purpose, a noxious mechanical stimulus and a control stimulus were applied in a randomized order. The recorded movements of the embryos were evaluated using the markerless pose estimation software DeepLabCut and manual observations. After the application of the mechanical stimulus, a significant increase in beak movement was identified in 15- to 18-day-old embryos. In younger embryos, no behavioral changes related to the noxious stimulus were observed. The presented results indicate that noxious mechanical stimuli at the beak base evoke a nocifensive reaction in chicken embryos starting at embryonic day 15.

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Xenografting Human Musculoskeletal Sarcomas in Mice, Chick Embryo, and Zebrafish: How to Boost Translational Research

Giusti,

Miserocchi,

Sbanchi

et al. 2024

Biomedicines

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Musculoskeletal sarcomas pose major challenges to researchers and clinicians due to their rarity and heterogeneity. Xenografting human cells or tumor fragments in rodents is a mainstay for the generation of cancer models and for the preclinical trial of novel drugs. Lately, though, technical, intrinsic and ethical concerns together with stricter regulations have significantly curbed the employment of murine patient-derived xenografts (mPDX). In alternatives to murine PDXs, researchers have focused on embryonal systems such as chorioallantoic membrane (CAM) and zebrafish embryos. These systems are time- and cost-effective hosts for tumor fragments and near-patient cells. The CAM of the chick embryo represents a unique vascularized environment to host xenografts with high engraftment rates, allowing for ease of visualization and molecular detection of metastatic cells. Thanks to the transparency of the larvae, zebrafish allow for the tracking of tumor development and metastatization, enabling high-throughput drug screening. This review will focus on xenograft models of musculoskeletal sarcomas to highlight the intrinsic and technically distinctive features of the different hosts, and how they can be exploited to elucidate biological mechanisms beneath the different phases of the tumor’s natural history and in drug development. Ultimately, the review suggests the combination of different models as an advantageous approach to boost basic and translational research.

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Nociception in chicken embryos, Part I: Analysis of cardiovascular responses to a mechanical noxious stimulus

Cited by 6 publications

References 52 publications

Nociception in chicken embryos, Part III: Analysis of movements before and after application of a noxious stimulus

Nociception in chicken embryos, Part III: Analysis of movements before and after application of a noxious stimulus

Nociception in Chicken Embryos, Part III: Analysis of Movements before and after Application of a Noxious Stimulus

Xenografting Human Musculoskeletal Sarcomas in Mice, Chick Embryo, and Zebrafish: How to Boost Translational Research

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