Noggin4 expression during chick embryonic development

Бородулин, А. В.; Eroshkin, Fedor M.; Bayramov, A. V.; Zaraisky, Andrey G.

doi:10.1387/ijdb.120020az

Cited by 11 publications

(7 citation statements)

References 14 publications

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“…Currently three subfamilies of the Noggin family are known: Noggin1, Noggin2, and Noggin4 [1][2][3][4][5]. The data obtained in our laboratory demonstrated that secreted regulators Noggin1 and Noggin2 were involved in the binding to the proper ligands and inhi bition of three essential signal cascades, BMP, Nodal/Activin, and Wnt/betaCatenin [6,7].…”

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confidence: 79%

The interaction of secreted proteins Noggin4 and Wnt8 from Xenopus laevis embryos

et al. 2016

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We demonstrated that the secreted protein Noggin4 from Xenopus laevis was capable of the in vitro binding to the secreted factor Wnt8, one of the ligands of the Wnt/betaCatenin signaling pathway. It was also shown that posttranslational modifications occurring during secretion of these proteins from the embryonic cells were necessary for their effective interaction. Also, we proposed a method for the preparation of physiologically active secreted morphogenic proteins from the intercellular space of the Xenopus laevis embryos.

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confidence: 79%

The interaction of secreted proteins Noggin4 and Wnt8 from Xenopus laevis embryos

et al. 2016

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“…Y‐27632, a potent Rho kinase inhibitor, is known to promote the neuronal differentiation of several types of stem cells, such as human embryonic stem cells, bone marrow mesenchymal stem cells, and periodontal ligament stem cells . Noggin protein is associated with the development of various tissues, such as bones, nerve tissue, and muscles, and plays key roles in promoting somite patterning in the developing embryo by regulating bone morphogenic protein 4 (BMP4) . Rho‐associated kinase inhibitor and Noggin have been shown to play a role in recovery of spinal cord injuries after transplantation of human embryonic stem cells, but their underlying mechanism of synergistic action has not been clarified .…”

Section: Introductionmentioning

confidence: 99%

“…[11][12][13] Noggin protein is associated with the development of various tissues, such as bones, nerve tissue, and muscles, and plays key roles in promoting somite patterning in the developing embryo by regulating bone morphogenic protein 4 (BMP4). 14 Rhoassociated kinase inhibitor and Noggin have been shown to play a role in recovery of spinal cord injuries after transplantation of human embryonic stem cells, but their underlying mechanism of synergistic action has not been clarified. 15 In this study, we aim to elucidate the mechanism underlying the synergistic effect of Y-27632 and Noggin on the proliferation and differentiation of SHEDs.…”

Section: Introductionmentioning

confidence: 99%

Synergistic effects of Rho kinase inhibitor Y‐27632 and Noggin overexpression on the proliferation and neuron‐like cell differentiation of stem cells derived from human exfoliated deciduous teeth

et al. 2019

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Stem cells from human exfoliated deciduous teeth (SHEDs) are highly proliferative, clonogenic, and multipotent stem cells with a neural crest cell origin. This property could be a desirable option for potential therapeutic applications. In this study, we focus on the effects of Rho kinase inhibitors Y‐27632 and Noggin on the proliferation of SHEDs and their differentiation into neuron‐like cells. SHEDs were extracted from 10 samples of deciduous teeth obtained from healthy children aged from 5 to 10. The passaged SHEDs were transfected with Noggin, Y‐27632, or their combination. By means of MTT and colony formation assays, the effects of Y‐27632 and Noggin on cell viability and colony formation were detected. Cellular morphology and neurosphere formation were observed under a microscope. Y‐27632 transfection in SHEDs showed enhanced cell viability, colony formation, and neurosphere formation indicating that Y‐27632 could promote cell proliferation of SHEDs. Furthermore, we observed that the SHEDs treated with Noggin in combination with Y‐27632 displayed typical neuron‐like cell morphology and reticular processes. Noggin or Y‐27632 alone or in combination induced obviously increased NSE, Nestin, and GFAP levels, which were highest in SHEDs treated with the combination of Noggin and Y‐27632. These findings suggest that Y‐27632 promotes the proliferation of SHEDs, and Y‐27632 and Noggin in combination have a synergistic effect on promoting differentiation of SHEDs into neuron‐like cells.

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“…New players include Activin, Follistatin, Derrière, Coco, Mad3, BMP, Noggin4, Syndecan2 and Fgf8 [28,[34][35][36][37][38][39][40][41][42][43][44][45]. Recently, other downstream factors have been demonstrated in unilateral function during LR determination, such as calcium signalling and retinoic acid [46][47][48][49][50][51][52][53][54].…”

Section: Introductionmentioning

confidence: 99%

From cytoskeletal dynamics to organ asymmetry: a nonlinear, regulative pathway underlies left–right patterning

McDowell

Rajadurai

Levin

2016

Phil. Trans. R. Soc. B

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One contribution of 17 to a theme issue 'Provocative questions in left -right asymmetry'. Consistent left -right (LR) asymmetry is a fundamental aspect of the bodyplan across phyla, and errors of laterality form an important class of human birth defects. Its molecular underpinning was first discovered as a sequential pathway of left-and right-sided gene expression that controlled positioning of the heart and visceral organs. Recent data have revised this picture in two important ways. First, the physical origin of chirality has been identified; cytoskeletal dynamics underlie the asymmetry of single-cell behaviour and patterning of the LR axis. Second, the pathway is not linear: early disruptions that alter the normal sidedness of upstream asymmetric genes do not necessarily induce defects in the laterality of the downstream genes or in organ situs. Thus, the LR pathway is a unique example of two fascinating aspects of biology: the interplay of physics and genetics in establishing largescale anatomy, and regulative (shape-homeostatic) pathways that correct molecular and anatomical errors over time. Here, we review aspects of asymmetry from its intracellular, cytoplasmic origins to the recently uncovered ability of the LR control circuitry to achieve correct gene expression and morphology despite reversals of key 'determinant' genes. We provide novel functional data, in Xenopus laevis, on conserved elements of the cytoskeleton that drive asymmetry, and comparatively analyse it together with previously published results in the field. Our new observations and meta-analysis demonstrate that despite aberrant expression of upstream regulatory genes, embryos can progressively normalize transcriptional cascades and anatomical outcomes. LR patterning can thus serve as a paradigm of how subcellular physics and gene expression cooperate to achieve developmental robustness of a body axis.This article is part of the themed issue 'Provocative questions in leftright asymmetry'.

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Noggin4 expression during chick embryonic development

Cited by 11 publications

References 14 publications

The interaction of secreted proteins Noggin4 and Wnt8 from Xenopus laevis embryos

The interaction of secreted proteins Noggin4 and Wnt8 from Xenopus laevis embryos

Synergistic effects of Rho kinase inhibitor Y‐27632 and Noggin overexpression on the proliferation and neuron‐like cell differentiation of stem cells derived from human exfoliated deciduous teeth

From cytoskeletal dynamics to organ asymmetry: a nonlinear, regulative pathway underlies left–right patterning

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