Noise-Induced Hearing Loss in Mice: Effects of High and Low Levels of Noise Trauma in CBA Mice

Amanipour, Reza M.; Liu, Hanjun; Duvey, Guillaume; Célanire, Sylvain; Walton, Joseph P.; Frisina, Robert D.

doi:10.1109/embc.2018.8512525

Cited by 9 publications

(11 citation statements)

References 18 publications

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“…5 A,C). The second paradigm is designed to induce a permanent threshold shift (PTS) 32 , 33 from which wildtype mice would not completely recover (Fig. 5 B).…”

Section: Resultsmentioning

confidence: 99%

“…Mice (P30) were exposed to 8-16 kHz octave-band noise, 100 dB SPL for 2 h to generate a temporary threshold shift (TTS) 30 , 31 . In separate experiments, mice were exposed to 8-16 kHz octave-band noise, 110 dB SPL for 3 h to generate a permanent threshold shift (PTS) 32 . Noise exposure was performed in a custom sound-proof booth with an RX6 processor (Tucker-Davis Technologies, TDT) as described previously 31 .…”

Section: Methodsmentioning

confidence: 99%

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Targeted deletion of the RNA-binding protein Caprin1 leads to progressive hearing loss and impairs recovery from noise exposure in mice

Nolan

Chen

Gonçalves³

et al. 2022

Sci Rep

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Cell cycle associated protein 1 (Caprin1) is an RNA-binding protein that can regulate the cellular post-transcriptional response to stress. It is a component of both stress granules and neuronal RNA granules and is implicated in neurodegenerative disease, synaptic plasticity and long-term memory formation. Our previous work suggested that Caprin1 also plays a role in the response of the cochlea to stress. Here, targeted inner ear-deletion of Caprin1 in mice leads to an early onset, progressive hearing loss. Auditory brainstem responses from Caprin1-deficient mice show reduced thresholds, with a significant reduction in wave-I amplitudes compared to wildtype. Whilst hair cell structure and numbers were normal, the inner hair cell-spiral ganglion neuron (IHC-SGN) synapse revealed abnormally large post-synaptic GluA2 receptor puncta, a defect consistent with the observed wave-I reduction. Unlike wildtype mice, mild-noise-induced hearing threshold shifts in Caprin1-deficient mice did not recover. Oxidative stress triggered TIA-1/HuR-positive stress granule formation in ex-vivo cochlear explants from Caprin1-deficient mice, showing that stress granules could still be induced. Taken together, these findings suggest that Caprin1 plays a key role in maintenance of auditory function, where it regulates the normal status of the IHC-SGN synapse.

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“…5 A,C). The second paradigm is designed to induce a permanent threshold shift (PTS) 32 , 33 from which wildtype mice would not completely recover (Fig. 5 B).…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Targeted deletion of the RNA-binding protein Caprin1 leads to progressive hearing loss and impairs recovery from noise exposure in mice

Nolan

Chen

Gonçalves³

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The first is designed to induce a temporary threshold shift (TTS) (Hesse et al, 2016; Kujawa & Liberman, 2009) from which wildtype mice are known to recover (Fig.4A, C). The second paradigm is designed to induce a permanent threshold shift (PTS) (Amanipour et al, 2018; Y. Wang, Hirose, & Liberman, 2002) from which wildtype mice would not completely recover (Fig.4B).…”

Section: Resultsmentioning

confidence: 99%

“…Mice at P30 were exposed to 8-16kHz octave-band noise at 100dB SPL for 2 hours to generate a temporary threshold shift (TTS) (Hesse et al, 2016; Kujawa & Liberman, 2009). In a separate experiment, Caprin1 +/+ and Caprin1 tm3d/tm3d mice were exposed to 8-16kHz octave-band noise at 110dB SPL for 3 hours to generate a permanent threshold shift (PTS) (Amanipour et al, 2018). Noise exposures was performed in a custom sound-proof booth with an RX6 processor (Tucker-Davis Technologies, TDT) as described previously (Hesse et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Targeted deletion of the RNA-binding protein Caprin1 leads to progressive hearing loss and impairs recovery from noise exposure in mice

Nolan

Chen

Ac³

et al. 2021

Preprint

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Cell cycle associated protein 1 (Caprin1) is an RNA-binding protein that can regulate the cellular post-transcriptional response to stress. It is a component of both stress granules and neuronal RNA granules and is implicated in neurodegenerative disease, synaptic plasticity and long-term memory formation. Our previous work suggested that Caprin1 also plays a role in the response of the cochlea to stress. Here, targeted inner ear-deletion of Caprin1 in mice leads to an early onset, progressive hearing loss. Auditory brainstem responses from Caprin1-deficient mice show reduced thresholds, with a significant reduction in wave-I amplitudes compared to wildtype. Whilst hair cell structure and numbers were normal, the inner hair cell-spiral ganglion neuron (IHC-SGN) synapse revealed abnormally large post-synaptic GluA2 receptor puncta, a defect consistent with the observed wave-I reduction. Unlike wildtype mice, mild-noise induced hearing threshold shifts in Caprin1-deficient mice did not recover. Oxidative stress triggered TIA-1/HuR positive stress granule formation in ex-vivo cochlear explants from Caprin1-deficient mice, showing that stress granules could still be induced. Taken together, these findings suggest that Caprin1 plays a key role in maintenance of auditory function, where it regulates the normal status of the IHC-SGN synapse.

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Auditory robustness and resilience in the aging auditory system of the desert locust

Austin,

Thomas,

Warren

2024

Neurobiology of Aging

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Noise-Induced Hearing Loss in Mice: Effects of High and Low Levels of Noise Trauma in CBA Mice

Cited by 9 publications

References 18 publications

Targeted deletion of the RNA-binding protein Caprin1 leads to progressive hearing loss and impairs recovery from noise exposure in mice

Targeted deletion of the RNA-binding protein Caprin1 leads to progressive hearing loss and impairs recovery from noise exposure in mice

Targeted deletion of the RNA-binding protein Caprin1 leads to progressive hearing loss and impairs recovery from noise exposure in mice

Auditory robustness and resilience in the aging auditory system of the desert locust

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