Non-adhesive contrast substrate for single-cell trapping and Raman spectroscopic analysis

Peethan, Alina; Aravind, M.; Chidangil, Santhosh; George, Sajan D.

doi:10.1039/d2lc00665k

Cited by 7 publications

(3 citation statements)

References 36 publications

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“…The exposure of oxygen plasma onto the cleaned quartz surface results in the formation of −OH groups on the surface and makes them highly hydrophilic (with a water contact angle, WCA ∼ 0°). The −OH bonds on the quartz surface facilitate the grafting of silicone oil onto the surface via hydrolysis and condensation reactions. , As a result of oil grafting, the hydrophilic quartz becomes hydrophobic with a nonadhesive behavior toward the water. The equilibrium water contact angle (WCA) of a 10 μL droplet on the oil-grafted surface is measured to be ∼107 ± 2°, as compared with the pristine quartz that exhibits a WCA of 58 ± 2° (Figure a,b).…”

Section: Resultsmentioning

confidence: 99%

A Wettability Contrast SERS Droplet Assay for Multiplexed Analyte Detection

Puravankara,

Manjeri,

Kulkarni

et al. 2024

Anal. Chem.

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Droplet assay platforms have emerged as a significant methodology, providing distinct advantages such as sample compartmentalization, high throughput, and minimal analyte consumption. However, inherent complexities, especially in multiplexed detection, remain a challenge. We demonstrate a novel strategy to fabricate a plasmonic droplet assay platform (PDAP) for multiplexed analyte detection, enabling surfaceenhanced Raman spectroscopy (SERS). PDAP efficiently splits a microliter droplet into submicroliter to nanoliter droplets under gravity-driven flow by wettability contrast between two distinct regions. The desired hydrophobicity and adhesive contrast between the silicone oil-grafted nonadhesive hydrophilic zone with gold nanoparticles is attained through (3-aminopropyl) triethoxysilane (APTES) functionalization of gold nanoparticles (AuNPs) using a scotch-tape mask. The wettability contrast surface facilitates the splitting of aqueous droplets with various surface tensions (ranging from 39.08 to 72 mN/m) into ultralow volumes of nanoliters. The developed PDAP was used for the multiplexed detection of Rhodamine 6G (Rh6G) and Crystal Violet (CV) dyes. The limit of detection for 120 nL droplet using PDAP was found to be 134 pM and 10.1 nM for Rh6G and CV, respectively. These results align with those from previously reported platforms, highlighting the comparable sensitivity of the developed PDAP. We have also demonstrated the competence of PDAP by testing adulterant spiked milk and obtained very good sensitivity. Thus, PDAP has the potential to be used for the multiplexed screening of food adulterants.

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Section: Resultsmentioning

confidence: 99%

A Wettability Contrast SERS Droplet Assay for Multiplexed Analyte Detection

Puravankara,

Manjeri,

Kulkarni

et al. 2024

Anal. Chem.

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“…The spontaneous splitting of a droplet into multiple smaller droplets and integration with various sensing platforms has shown great potential, especially when integrated with optical spectroscopic studies. 16,18 However, most of these studies were carried out on flat surfaces and thus limit real-life applications where the surfaces are non-flat. To the best of our knowledge, no studies have been reported on curved surfaces such as contact lenses that utilized them for tear fluid splitting via adhesion differences of the substrate or employed optical sensing for tear fluid constituent measurements.…”

Section: Introductionmentioning

confidence: 99%

A surface-engineered contact lens for tear fluid biomolecule sensing

George

2024

Lab Chip

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“…Bio-inspired surfaces are showing their potential in diverse areas including self-cleaning, water harvesting, water repellency, antibacterial, drag and friction reduction, anti-reflection, anti-corrosion, anti-fouling, structural coloration, thermal insulation, biosensing, etc. [13][14][15][16][17][18][19][20][21][22][23]…”

Section: Introductionmentioning

confidence: 99%

Advances in Superhydrophobic Coatings

2023

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Superhydrophobic coatings have attracted substantial scientific research in recent years due to their real-world applications in a variety of fields including anti-corrosion, anti-icing, anti-fogging, oil separation and self-cleaning materials. Several approaches and methods have been used in developing superhydrophobic coatings on different substrates. This book guides readers through their fabrication and application, representing the latest significant advances in this important topic. Split into four sections, Part One concentrates on the fundamental principles of surface wettability and superhydrophobicity, before moving into Part Two and Part Three, which focus on different aspects of superhydrophobic coatings based on the materials used for fabrication and the substrate on which the coating is applied. Finally, Part Four focuses on the applications of these coatings. This book will serve as a valuable reference for graduate students, researchers and industrial practitioners in multiple industries working on surface wettability, materials chemistry and coatings related technologies.

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Non-adhesive contrast substrate for single-cell trapping and Raman spectroscopic analysis

Abstract: Droplet splitting by exploiting the tailored surface wettability is emerging as an important pathway to create the ultralow volume of samples that can find applications in bioassays, tissue engineering, protein...

Cited by 7 publications

References 36 publications

A Wettability Contrast SERS Droplet Assay for Multiplexed Analyte Detection

A Wettability Contrast SERS Droplet Assay for Multiplexed Analyte Detection

A surface-engineered contact lens for tear fluid biomolecule sensing

Advances in Superhydrophobic Coatings

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