Non-canonical Phototransduction Mediates Synchronization of the Drosophila melanogaster Circadian Clock and Retinal Light Responses

Ogueta, Maite; Hardie, Roger; Stanewsky, Ralf

doi:10.1016/j.cub.2018.04.016

Cited by 45 publications

(76 citation statements)

References 61 publications

(121 reference statements)

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“…Interestingly, the phase advance is 10 rescued in the norpA P41 rdgA 1 double mutants ( Figure S2B), indicating that it is related to retinal degeneration observed in the rdgA single mutants ( Figure S1). As previously reported, norpA P41 cry 02 double mutants require 5 days for resynchronization ( Figure 3A, C, Table 1) [7]. Surprisingly, and consistent with the results from the LD experiment, rdgA 1 cry 02 double mutants completely fail to synchronize to the phase-delayed LD cycle ( Figure 3A, C; Table 1).…”

Section: Lack Of Rdga Function Rescues Behavioral Synchronization Of supporting

confidence: 90%

“…We confirm here the role of PLC21C for synchronization of the circadian clock to LD cycles (( Figure 5, [7]). Like in olfactory signal transduction, PLC21C light-resetting function requires Gq activation ([28], Figure 5) by Rhodopsin photopigments [7]. We show that in the absence of norpA and rdgA function, Plc21C mediates efficient Rhodopsin signaling to the clock neurons, so that resynchronization behavior is almost indistinguishable from that of flies with an intact visual system (Figures 3-5).…”

Section: Role Of Plc21c In the Drosophila Clockmentioning

confidence: 61%

“…Comparison of PER levels in the different neuronal groups and time points was performed as previously described [7]. Briefly, flies were entrained to LD and temperature cycles for four days, flowed by 6 hr delayed LD at constant 25°C as described above for the jetlag 22 experiments.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…Electroretinograms (ERG) were recorded as described previously (e.g. [7,56] Optical neutralization 24 Rhabomere integrity was assessed in young (1-2 day old) red-eyed flies as previously described (e.g. [24,57]) by observing rhabdomere patterns with antidromic white illumination under an 40x oil immersion objective focused below the cornea of decapitated heads fixed to a microscope slide using clear nail varnish.…”

Section: Erg Recordingsmentioning

confidence: 99%

“…Interestingly, the norpA-dependent and norpA-independent pathways contributing to light synchronization of the clock, also contribute to ERG responses of retinal photoreceptor cells to brief flashes of light [7]. Previously, it was shown that the almost complete lack of ERG responses in norpA mutants could be partially restored by simultaneous mutation of the rdgA gene, encoding DAG-kinase [21,22].…”

Section: Introductionmentioning

confidence: 99%

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Less is more: Light sampling via throttled visual phototransduction robustly synchronizes theDrosophilacircadian clock in the absence of Cryptochrome

Ogueta

Hardie

Stanewsky

2020

Preprint

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The daily changes of light and dark exemplify a prominent cue for the synchronization of internal circadian clocks to external time. The match between external and internal time is crucial for the fitness of organisms and desynchronization has been linked to numerous physical and mental health problems in humans. Organisms therefore developed complex and not fully understood mechanisms to synchronize their circadian clock to light. In mammals and in Drosophila both the visual system and dedicated non-image forming photoreceptors contribute to light resetting of the circadian clock. In the fruit fly, lightdependent degradation of the clock protein TIMELESS (TIM) by the blue light photoreceptor Cryptochrome is considered the main mechanism for clock synchronization, although the visual system also contributes. In order to understand the nature of the visual system contribution, we generated a genetic variant exhibiting extremely slow phototransduction kinetics, yet normal sensitivity. We show that in this variant the visual system is able to contribute its full share to circadian clock entrainment, both with regard to behavioral and molecular synchronization to light:dark cycles. This function depends on an alternative Phospholipase C-ß enzyme, encoded by PLC21C, presumably playing a dedicated role in clock resetting by light. We show that this pathway requires the ubiquitin ligase CULLIN-3, presumably mediating CRY-independent degradation of TIM during light:dark cycles. Our results suggest that visual system contribution to circadian clock entrainment operates on a drastically slower time scale compared with fast, visual and image forming phototransduction. Our findings are therefore consistent with the general idea that the visual system samples light over prolonged periods of time (hours) in order to reliably synchronize their internal clocks with the external time.

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Section: Lack Of Rdga Function Rescues Behavioral Synchronization Of supporting

confidence: 90%

Section: Role Of Plc21c In the Drosophila Clockmentioning

confidence: 61%

Section: Immunohistochemistrymentioning

confidence: 99%

Section: Erg Recordingsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Less is more: Light sampling via throttled visual phototransduction robustly synchronizes theDrosophilacircadian clock in the absence of Cryptochrome

Ogueta

Hardie

Stanewsky

2020

Preprint

Self Cite

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Light input pathways to the circadian clock of insects with an emphasis on the fruit fly Drosophila melanogaster

Helfrich‐Förster

2019

J Comp Physiol A

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Light is the most important Zeitgeber for entraining animal activity rhythms to the 24-h day. In all animals, the eyes are the main visual organs that are not only responsible for motion and colour (image) vision, but also transfer light information to the circadian clock in the brain. The way in which light entrains the circadian clock appears, however, variable in different species. As do vertebrates, insects possess extraretinal photoreceptors in addition to their eyes (and ocelli) that are sometimes located close to (underneath) the eyes, but sometimes even in the central brain. These extraretinal photoreceptors contribute to entrainment of their circadian clocks to different degrees. The fruit fly Drosophila melanogaster is special, because it expresses the blue light-sensitive cryptochrome (CRY) directly in its circadian clock neurons, and CRY is usually regarded as the fly's main circadian photoreceptor. Nevertheless, recent studies show that the retinal and extraretinal eyes transfer light information to almost every clock neuron and that the eyes are similarly important for entraining the fly's activity rhythm as in other insects, or more generally spoken in other animals. Here, I compare the light input pathways between selected insect species with a focus on Drosophila's special case.

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Circadian entrainment to red-light Zeitgebers and action spectrum for entrainment in the jewel wasp Nasonia vitripennis

Wang,

Jin,

Belušič

et al. 2023

J Comp Physiol A

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Light is the most important environmental cue for the circadian system of most organisms to stay synchronized to daily environmental changes. Like many other insects, the wasp Nasonia vitripennis has trichromatic compound eye-based colour vision and is sensitive to the light spectrum ranging from UV to green. We recently described a red-sensitive, ocelli-based photoreceptor, but its contribution to circadian entrainment remains unclear. In this study, we investigated the possibility of Nasonia circadian light entrainment under long-wavelength red LED light–dark cycles and characterized the strength of red light as a potential Zeitgeber. Additionally, we measured the possibility of entrainment under various light intensities (from 5·1012 to 4·1015 photons·cm−2·s−1) and a broader range of wavelengths (455—656 nm) to construct corresponding action spectra for characterizing all circadian photoreceptors involved in photic entrainment. We also conducted electroretinogram (ERG) recordings for each wavelength in the compound eyes. Our findings demonstrate that Nasonia can entrain under red light dark cycles, and the sensory pathway underlying the red-light Zeitgeber response may reside in the ocelli. Combined with findings from previous research, we pose that blue- and green-sensitive rhodopsin photoreceptor cells function as the major circadian photoreceptors in both circadian entrainment by light–dark cycles and circadian phase shifts by light pulses, whereas the red-sensitive photoreceptor cell requires higher light intensity for its role in circadian entrainment by light–dark cycles.

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Non-canonical Phototransduction Mediates Synchronization of the Drosophila melanogaster Circadian Clock and Retinal Light Responses

Cited by 45 publications

References 61 publications

Less is more: Light sampling via throttled visual phototransduction robustly synchronizes theDrosophilacircadian clock in the absence of Cryptochrome

Less is more: Light sampling via throttled visual phototransduction robustly synchronizes theDrosophilacircadian clock in the absence of Cryptochrome

Light input pathways to the circadian clock of insects with an emphasis on the fruit fly Drosophila melanogaster

Circadian entrainment to red-light Zeitgebers and action spectrum for entrainment in the jewel wasp Nasonia vitripennis

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