Non‐coding RNA MFI2‐AS1 promotes colorectal cancer cell proliferation, migration and invasion through miR‐574‐5p/MYCBP axis

Abstract

This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. AbstractObjective: Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) play essential roles in the tumour progression. LncRNAs mostly act as competing endogenous RNAs (ceRNAs) by sponging miRNAs. This study aimed to study the association of a novel lncRNA MFI2-AS1 with miR-574-5p/MYCBP axis in the development of colorectal cancer (CRC).Methods: Ninety-four CRC tissues and paired adjacent non-tumour tissues were included in our study. The relative expression level of MFI2-AS1 was detected, and its relationship with clinico-pathological factors was analysed. Then, the CRC cells lines (LoVo and RKO) were transfected with MFI2-AS1 siRNA, miR-574-5p mimics and inhibitors. Cell proliferation, migration, invasion, cell cycle distribution and DNA damage in response to different transfection conditions were examined. Dual-luciferase reporter assay was performed to identify the target interactions between MFI2-AS1 and miR-574-5p, miR-574-5p and MYCBP. Results: LncRNA MFI2-AS1 and MYCBP were up-regulated in CRC tissues when compared with adjacent non-tumour tissues. The expression levels of MFI2-AS1 were significantly associated with tumour histological grade, lymph and distant metastasis, TNM stage and vascular invasion. Both MFI2-AS1 siRNA and miR-574-5p mimics inhibited proliferation, migration and invasion in LoVo and RKO cells. The transfection of miR-574-5p inhibitor showed MFI2-AS1 siRNA-induced changes in CRC cells. Dual-luciferase reporter assay revealed target interactions between MFI2-AS1 and miR-574-5p, miR-574-5p and MYCBP. Conclusions: These findings suggested that lncRNA MFI2-AS1 and MYCBP have promoting effects in CRC tissues. LncRNA MFI2-AS1 promoted CRC cell proliferation, migration and invasion through activating MYCBP and by sponging miR-574-5p. S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section at the end of the article. How to cite this article: Li C, Tan F, Pei Q, et al. Non-coding RNA MFI2-AS1 promotes colorectal cancer cell proliferation, migration and invasion through miR-574-5p/MYCBP axis. Cell

LncRNA LINC00342 contributes to the growth and metastasis of colorectal cancer via targeting miR-19a-3p/NPEPL1

“…For example, Xu et al reported that lncRNA TUSC7 functioned as a miR-211 decoy in the regulation of CDK6SH3 and suppressed the proliferation of CRC cells [16]. Li et al exhibited that lncRNA MFI2-AS1 regulated CRC cell proliferation, metastasis, cell cycle distribution, and apoptosis through competitively interacting with miR-574-5p to upregulate MYCBP expression [17]. Shi et al found that ZNFX1-AS1 served as a ceRNA to upregulate EZH2 expression by sponging miR-144 to promote CRC progression [18].…”

LncRNA LINC00342 contributes to the growth and metastasis of colorectal cancer via targeting miR-19a-3p/NPEPL1

“…For example, Xu et al has reported that lncRNA TUSC7 functions as a miR-211 decoy in the regulation of CDK6SH3 and suppresses the proliferation of CRC cells [28]. Li et al has exhibited that lncRNA MFI2-AS1 regulates CRC cell proliferation, metastasis, cell cycle distribution, and apoptosis through competitively interacting with miR-574-5p to upregulate MYCBP expression [29]. Shi et al nds that ZNFX1-AS1 serves as a ceRNA to upregulate EZH2 expression by sponging miR-144 to promote CRC progression [30].…”

Identification of differentially expressed mRNA and the Hub mRNAs modulated by lncRNA Meg3 as a competing endogenous RNA in brown adipose tissue of mice on a high-fat diet

“…MiRNAs are short RNAs that can be regulated by the sponge role of lncRNA, following by modulating the expression of the downstream genes by targeting their 3′untranslated regions (3′UTR) [35]. An increasing number of studies has demonstrated that miR-574-5p can be sponged by lncRNA, like lncRNA PTCSC3 and lncRNA MFI2-AS1, to modulate growth and metastasis of cancer cells [36,37]. In addition, miR-770-5p has been found to be downregulated by lncRNA TPT1-AS1 and correspondingly upregulated the expression of STMN1, leading to promotion of the proliferation of Glioma cells [38].…”