Non-coding RNAs in hepatocellular carcinoma: Insights into regulatory mechanisms, clinical significance, and therapeutic potential

Qin, Han; Wang, Mengchen; Dong, Xiaoli; Wei, Fei; Luo, Yun; Sun, Xiaodong

doi:10.3389/fimmu.2022.985815

Cited by 18 publications

(10 citation statements)

References 232 publications

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“…However, the specific mechanism of HCC development is still controversial, and most scholars believe that its development is highly correlated with circRNA [14]. circRNA is a special non-coding RNA [15], mainly involved in gene expression regulation at the transcriptional and post-transcriptional levels. By regulating the expression of downstream target genes, mirnas and proteins, CircrNA can affect the proliferation [16], migration, invasion and apoptosis of tumor cells, etc., thus affecting the occurrence and development of HCC.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA Circ_0001971 Aggravates the Progression of Hepatocellular Carcinoma Cells by Activating the miR-186-5p/KLF7

Zeng¹

2023

IJCEMR

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Background: Circ_0001971 has been shown to play an important role in cancer development, yet its mechanism of action in hepatocellular carcinoma (HCC) remains somewhat unknown. Methods: RNA sequence analysis was performed on HCC tissues and paired non-tumor tissues to detect differentially expressed Rnas. qPCR was used to detect the expression level of circ_0001971. CCK-8, Transwell, RNA immunoprecipitation experiment, WB detection, Double luciferase reporter gene assay et al. were used to analyze the interaction between circ_0001971 and miR-186-5p/KLF7. Results: Circ_0001971 expression was significantly upregulated in HCC cells; circ_0001971 inhibition significantly inhibited proliferation, migration and invasion of HCC cells (P<0.05); miR-186-5p was a downstream target of circ_0001971; KLF7 was a target gene of miR-186-5p; circ_0001971 can indirectly positively regulate KLF7. Conclusions: Circ_0001971 aggravates HCC progression by activating miR-186-5p/KLF7.

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Section: Discussionmentioning

confidence: 99%

Circular RNA Circ_0001971 Aggravates the Progression of Hepatocellular Carcinoma Cells by Activating the miR-186-5p/KLF7

Zeng¹

2023

IJCEMR

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“…Noncoding RNA (regulatory ncRNAs) involved in the regulation of the same type of gene are known as miRNAs. There are two main types of ncRNAs: long noncoding RNAs (lncRNAs) and short noncoding RNAs (piRNAs, miRNAs, and siRNAs) . lncRNAs and miRNAs interact as competitive endogenous RNAs (ceRNAs), which allows the former to regulate the expression and biological functions of miRNAs .…”

Section: Discussionmentioning

confidence: 99%

“…There are two main types of ncRNAs: long noncoding RNAs (lncRNAs) and short noncoding RNAs (piRNAs, miRNAs, and siRNAs). 41 lncRNAs and miRNAs interact as competitive endogenous RNAs (ceRNAs), which allows the former to regulate the expression and biological functions of miRNAs. 42 Lu et al, discovered that the lncRNA HOTAIR targeted miR-204-5p in cholangiocarcinoma cells, thereby inhibiting apoptosis and autophagy and promoting cell proliferation.…”

Section: ■ Discussionmentioning

confidence: 99%

MicroRNA-204-5p Inhibits Hepatocellular Carcinoma by Targeting the Regulator of G Protein Signaling 20

Su,

Lu,

et al. 2023

ACS Pharmacol. Transl. Sci.

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Although the oncogenic roles of regulator of G protein signaling 20 (RGS20) and its upstream microRNAs (miRNAs) have been reported, their involvement in hepatocellular carcinoma (HCC) remains unexplored. We utilized the starBase, miRDB, TargetScan, and mirDIP databases, along with a dual-luciferase reporter assay and cDNA chip analysis to identify miRNAs targeting RGS20. miR-204-5p was selected for further experiments to confirm its direct targeting and downregulation of the RGS20 expression. To study the miR-204-5p/RGS20 axis in HCC, RGS20 and miR-204-5p were increased in PLC/PRF/5/Hep3B cells, and the viability, hyperplasia, apoptosis, cell cycle, and invasion/migration of the cells were assessed. RGS20 exhibited optimism, while miR-204-5p exhibited pessimism in tumors. miR-204-5p directly targeted RGS20 and downregulated its expression, whereas high RGS20 expression indicated a poor prognosis. Transfection of miR-204-5p inhibited the hyperplasia, migration, and invasion of HCC cells, but promoted apoptosis and influenced the levels of cyclin-dependent kinase 2 (CDK2), cyclin E1, B-cell lymphoma-2 (Bcl-2), Bax, and cleaved caspase-3/8. These effects were reversed by overexpression of RGS20. We recognized miR-204-5p as an upstream regulator targeting RGS20, thereby inhibiting HCC progression by downregulating RGS20 expression. RGS20 may prove to be a potential target for HCC treatment, and miR-204-5p might seem like to be a potential miRNA in gene therapy.

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“…Unlike sampling ncRNAs via invasive techniques such as biopsy, it is easy and inexpensive to detect ncRNAs from blood samples ( 119 ). However, the current obstacle is the lack of some ncRNAs with sensitivity and specificity ( 120 ).…”

Section: Prognostic and Therapeutic Targetsmentioning

confidence: 99%

Crosstalk between ubiquitin ligases and ncRNAs drives cardiovascular disease progression

You,

Wen,

Tian

et al. 2024

Front. Immunol.

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Cardiovascular diseases (CVDs) are multifactorial chronic diseases and have the highest rates of morbidity and mortality worldwide. The ubiquitin–proteasome system (UPS) plays a crucial role in posttranslational modification and quality control of proteins, maintaining intracellular homeostasis via degradation of misfolded, short-lived, or nonfunctional regulatory proteins. Noncoding RNAs (ncRNAs, such as microRNAs, long noncoding RNAs, circular RNAs and small interfering RNAs) serve as epigenetic factors and directly or indirectly participate in various physiological and pathological processes. NcRNAs that regulate ubiquitination or are regulated by the UPS are involved in the execution of target protein stability. The cross-linked relationship between the UPS, ncRNAs and CVDs has drawn researchers’ attention. Herein, we provide an update on recent developments and perspectives on how the crosstalk of the UPS and ncRNAs affects the pathological mechanisms of CVDs, particularly myocardial ischemia/reperfusion injury, myocardial infarction, cardiomyopathy, heart failure, atherosclerosis, hypertension, and ischemic stroke. In addition, we further envision that RNA interference or ncRNA mimics or inhibitors targeting the UPS can potentially be used as therapeutic tools and strategies.

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Non-coding RNAs in hepatocellular carcinoma: Insights into regulatory mechanisms, clinical significance, and therapeutic potential

Cited by 18 publications

References 232 publications

Circular RNA Circ_0001971 Aggravates the Progression of Hepatocellular Carcinoma Cells by Activating the miR-186-5p/KLF7

Circular RNA Circ_0001971 Aggravates the Progression of Hepatocellular Carcinoma Cells by Activating the miR-186-5p/KLF7

MicroRNA-204-5p Inhibits Hepatocellular Carcinoma by Targeting the Regulator of G Protein Signaling 20

Crosstalk between ubiquitin ligases and ncRNAs drives cardiovascular disease progression

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