Non contiguous-finished genome sequence and description of Alistipes obesi sp. nov

Abstract

Alistipes obesi sp. nov. strain ph8T is the type strain of A. obesi, a new species within the genus Alistipes. This strain, whose genome is described here, was isolated from the fecal flora of a 26-year-old woman suffering from morbid obesity. A. obesi is an obligately anaerobic rod. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 3,162,233 bp long genome (1 chromosome but no plasmid) contains 2,623 protein-coding and 49 RNA genes, including three rRNA genes.

Noncontiguous finished genome sequence and description of Paenibacillus ihumii sp. nov. strain AT5

“…The time of acquisition was between 30 seconds and 1 minute per spot. Identification was carried out as previously reported [10] .…”

Butyricimonas phoceensis sp. nov., a new anaerobic species isolated from the human gut microbiota of a French morbidly obese patient

“…Briefly, one isolated bacterial colony was picked up with a pipette tip from a culture agar plate and spread as a thin smear on a MTP 384 MALDI-TOF MS target plate (Bruker Daltonics, Leipzig, Germany). Measurement and identification were performed as previously described [16] . When a bacterium was unidentifiable, 16S rRNA gene amplification and sequencing were performed.…”

Alistipes inops sp. nov. and Coprobacter secundus sp. nov., isolated from human faeces

“…This applies fully to the genus Alistipes , family Rikenellaceae , the type species of which, Alistipes putredinis (Rautio et al , 2003), was formerly known as Bacteroides putredinis (Weinberg et al , 1937). In recent years, the genus Alistipes has been expanded by the addition of a number of newly described and reclassified species, including Alistipes finegoldii (Rautio et al , 2003), Alistipes onderdonkii (Song et al , 2006), Alistipes shahii (Song et al , 2006), Alistipes indistinctus (Nagai et al , 2010), ‘ Alistipes senegalensis ’ (Mishra et al , 2012), Alistipes timonensis (Lagier et al , 2012b), ‘ Alistipes obesi ’ (Hugon et al , 2013) and ‘ Alistipes ihumii ’ (Pfleiderer et al , 2014). Most species of Alistipes were isolated from the healthy human GI tract microbiota, where they can be found in relatively high titres exceeding 10 9 c.f.u.…”

“…In addition, cases have been reported of isolation of members of the genus Alistipes from blood, appendicular, abdominal, perirectal and brain abscesses, urine and intra-abdominal fluid (Song et al , 2006). Interestingly, the proposed type strain of ‘ A. obesi ’ was isolated from the stool of a patient suffering from morbid obesity (Hugon et al , 2013), while ‘ A. ihumii ’ was discovered in the faecal microbiota of an anorexia nervosa patient (Pfleiderer et al , 2014).…”