Non-covalent binding and selective fluorescent sensing of dipyrone with a carbocyanine dye and cetyltrimethylammonium bromide

Abstract

The work is aimed at the search of selective fluorescent sensors without using specific artificial receptors, antibodies, enzymes etc. With this end in view, methods based on non-covalent binding of target analytes are sought. We observed dramatic changes in the emission spectrum of a carbocyanine dye in a micellar surfactant solution (cetyltrimethylammonium bromide, CTAB) in the presence of dipyrone (metamizol, analgin): the 480 nm band intensity increases with a simultaneous decrease in intensity in near-IR region (720 nm). MALDI and NMR-1H data show the intact molecules of dipyrone and the dye. The detection can be performed in the presence of other organic species and inorganic salts. Dipyrone testing is feasible within 5 × 10–7–5 × 10–4 M with RSDs of 3.5% by using a visualizer instead of a spectrofluorimeter.