Non-destructive hydrocarbon extraction from Botryococcus braunii BOT-22 (race B)

Moheimani, Navid R.; Matsuura, Hiroshi; Watanabe, Makoto M.; Borowitzka, Michael A.

doi:10.1007/s10811-013-0179-0

Cited by 56 publications

(31 citation statements)

References 32 publications

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“…Means ± SD. P/I curve = photosynthesis-irradiance curve.The parameters Fv/Fm and ETRmax are commonly used to evaluate the impact of stresses such as nutrient or heat stress on microalgae[32,33]. In our experimental conditions, the absence of any sharp decline in Fv/Fm and ETR max values suggests B. braunii were not 0…”

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confidence: 86%

Extracellular hydrocarbon and intracellular lipid accumulation are related to nutrient-sufficient conditions in pH-controlled chemostat cultures of the microalga Botryococcus braunii SAG 30.81

et al. 2016

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International audienceHere we investigate the effect of environmental pH on biomass and hydrocarbon productivity in photoautotro-phic cultures of Botryococcus braunii SAG 30.81 (race A). Successive steady-states of continuous cultures were used to study the effects of pH controlled by carbon dioxide feeds in a closed photobioreactor. At a fixed dilution rate of 0.2 d −1 , hydrocarbon productivity was found to be pH-dependent in the range 5.5 to 8.0, with an optimum at pH 6.5. pH variations from pH 6.0 to pH 8.0 had little effect on biomass productivity, nutrient requirement for biomass production, photosynthetic yield and pigment ratios, whereas acidic pH 5.5 conditions induced a slight decrease in biomass and hydrocarbon productivities as well as stressing the cells. The pH effect occurred even after a threefold increase in nitrate and phosphate concentrations in the feed culture medium, resulting in bio-mass productivity up to 8.9 g m −2 d −1 and hydrocarbon productivity up to 0.5 g m −2 d −1. Dissolved inorganic carbon data suggest that a maximum hydrocarbon content of 5.7% dry weight was reached at and above 1 mmol L −1 dissolved CO 2 and that hydrocarbon production was linearly related to CO 2 up to 2.5 mmol L −1 , whatever the N and P enrichment of AF-6 culture medium

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confidence: 86%

Extracellular hydrocarbon and intracellular lipid accumulation are related to nutrient-sufficient conditions in pH-controlled chemostat cultures of the microalga Botryococcus braunii SAG 30.81

et al. 2016

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“…Unfortunately, several problems were found from these methods. The hydrocarbon yields from pressurized colonies were low, the heating methods involved long extraction times, and the organic solvent pretreatment studied by Kanda et al consumed more energy than supercritical CO 2 extraction . Thus, none of these methods is practical for commercial biofuel production.…”

Section: Introductionmentioning

confidence: 99%

Mechanical cell disruption of microalgae for investigating the effects of degree of disruption on hydrocarbon extraction

Tsutsumi

Yokomizo

Saito

et al. 2017

Asia-Pacific J Chem Eng

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The colonial microalga, Botryococcus braunii, produces and stores hydrocarbons in membranes and in colonies. Although hydrocarbons in B. braunii could easily be extracted for biofuel production, yields are actually poor without energy-intensive pretreatment such as thermal drying. To develop extraction methods without drying, we applied the mechanical cell disruption to wet B. braunii using either a high-pressure homogenizer, a bead mill, or a circulating particle disruptor (Jet-Paster) and examined the relationships between the extent of cell and/or colony disruption and extracted hydrocarbon yields using n-hexane. When the number of particles over 20 μm classified as colonies, decreased after each treatment, the hydrocarbon yields of samples treated (over 20%) were much larger than those without treatments (under 5%). Thus, the colony disruption may increase hydrocarbon yields by enhancing n-hexane penetration to the colony. Although the degree of disruption on the Jet-Paster treatment was the lowest (2.2-9.3%) and that of sample treated by the homogenizer was the highest (27-55%), extracted hydrocarbon yields were improved in both treatments. The facts indicate that disrupting large colonies into small colonies improves hydrocarbon recovery, and the fractionation of cells is not needed for hydrocarbon extraction from B. braunii. Figure 4. Distributions of roundness of before and after cell disruption ((a-1), (b-1), and (c-1)) Untreated samples. (a-2) High-pressure homogenizer (30 MPa). (a-3) High-pressure homogenizer (80 MPa). (b-2) Bead mill (8 m/s). (b-3) Bead mill (14 m/s). (c-2) Jet-Paster (4800 rpm). (c-3) Jet-Paster (7200 rpm). S. TSUTSUMI ET AL.Asia-Pacific Journal of Chemical Engineering 460

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“…Por su parte, Metzger et al [26] reportaron incrementos en el contenido de hidrocarburos desde el 5% para cultivos sin aire, hasta el 61% para cultivos con suministro de aire mezclado con CO 2 al 1%, dependiendo del origen de la cepa. Moheimani et al [45] estudiaron la recuperación de hidrocarburos en cultivos de B.braunii después de una primera extracción, encontrando tiempos de 11 días para cultivos sin suministro de CO 2 y de 4 días para cultivos con suministro de 0,02v/vm. En el presente estudio se encontraron las productividades más altas de hidrocarburos usando las concentraciones más bajas de CO 2 .…”

Section: Análisis De Resultadosunclassified

Desarrollo preliminar de una metodología de suministro de CO2 a cultivos de Botryococcus Braunii para la producción de biocombustibles

Villarreal¹,

Kafarov²

2015

rev.ion

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ResumenDiversos estudios sobre el cultivo de microalgas a escala laboratorio y piloto han registrado el potencial de estos microorganismos para la producción de materia prima en la elaboración de biocombustibles. El suministro de una fuente de carbono para el cultivo masivo de microalgas representa una de las principales limitantes en el proceso productivo; estas fi jan fotosintéticamente carbono inorgánico (CO 2 ) y sintetizan metabolitos para la producción de biocombustibles. En el presente estudio se evaluó la infl uencia de diversos parámetros de suministro de CO 2 sobre cultivos de la especie Botryococcus braunii en fotobiorreactores a escala laboratorio. Se plantearon diseños experimentales centrales compuestos para correlacionar los parámetros a evaluar y determinar su efecto en la dilución de CO 2 y producción de metabolitos (biomasa, lípidos e hidrocarburos). Respecto a los parámetros de diseño del fotobiorreactor, alturas > 36cm, diámetros < 7cm y pequeños tamaños de burbuja ayudan a incrementar la dilución del CO 2 hasta en 180%. Se encontró que altas concentraciones de CO 2 (> 0,06v/vm) suministrado de manera continua son ideales para el crecimiento celular. Por otra parte, concentraciones medias (0,04 -0,06v/vm) de CO 2 son ideales para la producción de lípidos y concentraciones bajas de CO 2 (≤ 0,02v/vm) favorecen la producción de hidrocarburos. Se validó el potencial de la cepa de B. braunii colombiana para la producción de biodiésel gracias a sus altas tasas de síntesis de lípidos. AbstractSeveral studies about the cultivation of microalgae in laboratory and pilot scale have recorded the potential of these microorganisms for the production of raw material in the fabrication of biofuels. The provision of a carbon source for the mass cultivation of microalgae is one of the main constraints in the production process; these photosynthetically fi xed inorganic carbon (CO 2 ) and synthesized metabolites for biofuel production. In the present study the infl uence of various parameters of CO 2 supply over Botryococcus braunii crops in laboratory scale photobioreactors was evaluated. Central composite experimental 33 Cita: Jaimes Villareal NA, Kafarov V. Desarrollo preliminar de una metodología de suministro de CO 2 a cultivos de Botryococcus Braunii para la producción de biocombustibles. rev.ion. 2015;28(2):33-50.

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Non-destructive hydrocarbon extraction from Botryococcus braunii BOT-22 (race B)

Cited by 56 publications

References 32 publications

Extracellular hydrocarbon and intracellular lipid accumulation are related to nutrient-sufficient conditions in pH-controlled chemostat cultures of the microalga Botryococcus braunii SAG 30.81

Extracellular hydrocarbon and intracellular lipid accumulation are related to nutrient-sufficient conditions in pH-controlled chemostat cultures of the microalga Botryococcus braunii SAG 30.81

Mechanical cell disruption of microalgae for investigating the effects of degree of disruption on hydrocarbon extraction

Desarrollo preliminar de una metodología de suministro de CO2 a cultivos de Botryococcus Braunii para la producción de biocombustibles

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