Non-dividing Cell Virtosomes Affect In Vitro and In Vivo Tumour Cell Replication

García‐Arranz, Mariano; García-Olmo, Damián; Vega-Clemente, Luz; Stroun, Maurice; Gahan, P. B.

doi:10.1007/978-3-319-42044-8_9

Cited by 8 publications

(9 citation statements)

References 3 publications

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“…The production of exosomes has been shown to be markedly elevated in cancer [ 98 ], which can affect recipient cells in a variety of ways, e.g., development of acquired resistance to chemotherapeutic agents [ 99 ]. In contrast to the above observations, one in vitro study suggests that the lateral transfer of cfDNA derived from healthy cells can potentially halt the proliferation of cancer cells [ 100 ]. However, this requires further experimental verification.…”

Section: Roles Of Cfdna In the Pathology Of Cancermentioning

confidence: 99%

The emerging role of cell-free DNA as a molecular marker for cancer management

Bronkhorst

Ungerer

Holdenrieder

2019

Biomolecular Detection and Quantification

454

375

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An increasing number of studies demonstrate the potential use of cell-free DNA (cfDNA) as a surrogate marker for multiple indications in cancer, including diagnosis, prognosis, and monitoring. However, harnessing the full potential of cfDNA requires (i) the optimization and standardization of preanalytical steps, (ii) refinement of current analysis strategies, and, perhaps most importantly, (iii) significant improvements in our understanding of its origin, physical properties, and dynamics in circulation. The latter knowledge is crucial for interpreting the associations between changes in the baseline characteristics of cfDNA and the clinical manifestations of cancer. In this review we explore recent advancements and highlight the current gaps in our knowledge concerning each point of contact between cfDNA analysis and the different stages of cancer management.

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Section: Roles Of Cfdna In the Pathology Of Cancermentioning

confidence: 99%

The emerging role of cell-free DNA as a molecular marker for cancer management

Bronkhorst

Ungerer

Holdenrieder

2019

Biomolecular Detection and Quantification

454

375

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“…() and Garcia‐Arranz et al . (), who showed that virtosomes from non‐dividing cell populations can reduce tumour size in tumour‐bearing BDIX rats. Due to the presence of proteins in virtosomes, histological analysis of a range of tissues was performed and it was confirmed that proteins from the virtosomes were not responsible for the anti‐tumour effects.…”

Section: Sources Of Circulating Cfdnamentioning

confidence: 99%

“…A similar form of homeostasis may occur between populations of cells in order to equilibrate or manage genetic and/or epigenetic changes throughout tissues, organs and/or the body (Aucamp et al, ). The fact that ( i ) virtosomes have been found readily to enter other cells in vitro and in vivo and modify the biology of the recipient cells (Adams, Diaz & Gahan, ; García‐Olmo, Ruiz‐Piqueras & García‐Olmo, ; García‐Olmo et al, , ; Garcia‐Arranz et al, ) and ( ii ) cfDNA has been found to contain information/mutations similar to those of parental cells and can transfer this information between cells, e.g. chemoresistance (Chen et al, ), indicates that the messaging role of cfDNA in virtosomes and vesicles may indeed have homeostatic properties (Fig.…”

Section: Sources Of Circulating Cfdnamentioning

confidence: 99%

The diverse origins of circulating cell‐free DNA in the human body: a critical re‐evaluation of the literature

et al. 2018

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Since the detection of cell-free DNA (cfDNA) in human plasma in 1948, it has been investigated as a non-invasive screening tool for many diseases, especially solid tumours and foetal genetic abnormalities. However, to date our lack of knowledge regarding the origin and purpose of cfDNA in a physiological environment has limited its use to more obvious diagnostics, neglecting, for example, its potential utility in the identification of predisposition to disease, earlier detection of cancers, and lifestyle-induced epigenetic changes. Moreover, the concept or mechanism of cfDNA could also have potential therapeutic uses such as in immuno- or gene therapy. This review presents an extensive compilation of the putative origins of cfDNA and then contrasts the contributions of cellular breakdown processes with active mechanisms for the release of cfDNA into the extracellular environment. The involvement of cfDNA derived from both cellular breakdown and active release in lateral information transfer is also discussed. We hope to encourage researchers to adopt a more holistic view of cfDNA research, taking into account all the biological pathways in which cfDNA is involved, and to give serious consideration to the integration of in vitro and in vivo research. We also wish to encourage researchers not to limit their focus to the apoptotic or necrotic fraction of cfDNA, but to investigate the intercellular messaging capabilities of the actively released fraction of cfDNA and to study the role of cfDNA in pathogenesis.

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“…cfDNA affects many parameters of the organism. Circulating cfDNA can increase oxidative stress, stimulate the synthesis of proinflammatory cytokines, induce sterile inflammation, affect platelet activation, plasmatic coagulation, and fibrinolysis, and activate a large number of signaling pathways [24, 25].…”

Section: Circulating Cell-free Dnamentioning

confidence: 99%

Oxidized Cell-Free DNA Is a Factor of Stress Signaling in Radiation-Induced Bystander Effects in Different Types of Human Cells

Konkova

Kaliyanov

Sergeeva

et al. 2019

International Journal of Genomics

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In pathology or under damaging conditions, the properties of cell-free DNA (cfDNA) change. An example of such change is GC enrichment, which drastically alters the biological properties of cfDNA. GC-rich cfDNA is a factor of stress signaling, whereas genomic cfDNA is biologically inactive. GC-rich cfDNA stimulates TLR9-MyD88-NF-κB signaling cascade, leading to an increase in proinflammatory cytokine levels in the organism. In addition, GC-rich DNA is prone to oxidation and oxidized cfDNA can stimulate secondary oxidative stress. This article is a review of works dedicated to the investigation of a low-dose ionizing radiation effect, a bystander effect, and the role of cfDNA in both of these processes.

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Non-dividing Cell Virtosomes Affect In Vitro and In Vivo Tumour Cell Replication

Abstract: In vitro studies of partially purified virtosomes from rat liver showed inhibition of cell multiplication in four normal and two tumour cell lines. In vivo, the liver virtosomes slowed tumour growth and limited metastases in rats bearing DHD/K12-PROb cell initiated tumours.

Cited by 8 publications

References 3 publications

The emerging role of cell-free DNA as a molecular marker for cancer management

The emerging role of cell-free DNA as a molecular marker for cancer management

The diverse origins of circulating cell‐free DNA in the human body: a critical re‐evaluation of the literature

Oxidized Cell-Free DNA Is a Factor of Stress Signaling in Radiation-Induced Bystander Effects in Different Types of Human Cells

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