Non-endoscopic method to obtain Helicobacter pylori for culture

Pérez-Trallero, Emilio; Montes, Milagrosa; Alcorta, Marta Duque; Zubillaga, P.; Telleria, E.

doi:10.1016/s0140-6736(95)90524-3

Cited by 45 publications

(35 citation statements)

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“…Gastric juice allows the detection of H. pylori by culture, staining, urease test, and PCR, but it is less reliable than gastric biopsy specimens. The string test can also be used to obtain gastric mucus (443); however, the most attractive method seems to be an extendable oro-gastric brush contained in a plastic tube (Baylor Brush, US Endoscopy, TX). The brush is swallowed, extended into the stomach to brush the mucosa three or four times, retracted in the protective sleeve, and withdrawn from the patient.…”

Section: Invasive Testsmentioning

confidence: 99%

Helicobacter pyloriDetection and Antimicrobial Susceptibility Testing

2007

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SUMMARY The discovery of Helicobacter pylori in 1982 was the starting point of a revolution concerning the concepts and management of gastroduodenal diseases. It is now well accepted that the most common stomach disease, peptic ulcer disease, is an infectious disease, and all consensus conferences agree that the causative agent, H. pylori, must be treated with antibiotics. Furthermore, the concept emerged that this bacterium could be the trigger of various malignant diseases of the stomach, and it is now a model for chronic bacterial infections causing cancer. Most of the many different techniques involved in diagnosis of H. pylori infection are performed in clinical microbiology laboratories. The aim of this article is to review the current status of these methods and their application, highlighting the important progress which has been made in the past decade. Both invasive and noninvasive techniques will be reviewed.

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Section: Invasive Testsmentioning

confidence: 99%

Helicobacter pyloriDetection and Antimicrobial Susceptibility Testing

2007

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“…Additionally, a noninvasive string test has also been reported useful to obtain gastric juice samples for the diagnosis of H. pylori infection (6). This technique facilitates epidemiologic studies for assessing H. pylori status in asymptomatic subjects without the need for endoscopy (7).…”

mentioning

confidence: 99%

α1-Antitrypsin Precursor in Gastric Juice Is a Novel Biomarker for Gastric Cancer and Ulcer

Hsu

Chen

Hsieh

et al. 2007

Clinical Cancer Research

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Purpose: To search for novel disease-specific markers in gastric juice by investigating the protein concentrations and components in gastric juice from patients with various gastroduodenal diseases. Experimental Design: Protein concentrations and pH values in fasting gastric juice were examined in 120 healthy subjects and 39 gastric ulcer, 38 duodenal ulcer, and 31 gastric cancer patients. The protein components in gastric juice were studied by two-dimensional PAGE and mass spectrometric analysis. Results: Protein concentrations in gastric juice of patients with gastric ulcers and gastric cancer were significantly higher than those in healthy subjects (1.06 and 2.61 mg/mL versus 0.48 mg/mL; P = 0.001and P < 0.001, respectively), and duodenal ulcer patients had lower gastric juice protein concentrations compared with healthy subjects (0.26 versus 0.48 mg/mL; P < 0.05). Gastric hypoacidity and advanced age were independent factors affecting the protein concentrations in gastric juice with odds ratios of 32.9 (95% confidence interval, 11.8-90.9) and 3.2 (95% confidence interval, 1.3-8.3), respectively. Each electrophoresis images of gastric juice could be classified into one of three patterns: basic band, specific band, or nonspecific band. The frequencies of specific band pattern in healthy subjects, gastric ulcer, duodenal ulcer, and gastric cancer patients were 6%, 42%, 6%, and 93%, respectively. Proteomic analysis revealed that a1-antitrypsin precursor was the principal peptide in the specific band. Conclusions: a1-antitrypsin precursor in gastric juice is a novel biomarker for gastric cancer and ulcer. A noninvasive method to obtain gastric juice followed by proteomic analysis may serve as a new tool to screen for gastric malignancies.

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“…Various noninvasive methods have recently been developed for obtaining gastric juices (13)(14)(15). For example, a noninvasive string test has been reported to get gastric juice samples for the diagnosis of Helicobacter pylori infection (13,14).…”

Section: Introductionmentioning

confidence: 99%

“…For example, a noninvasive string test has been reported to get gastric juice samples for the diagnosis of Helicobacter pylori infection (13,14). Additionally, a capsule method containing a pierced plastic cover and a piece of absorbent paper has also been developed to get gastric juice (15).…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of Gastric Malignancy Using Gastric Juice α1-antitrypsin

Hsu

Chen

Hsiao

et al. 2010

Cancer Epidemiology, Biomarkers &Amp; Prevention

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No accurate, inexpensive, and noninvasive test for gastric cancer screening is currently available. Our recent study identified α1-antitrypsin as a potential biomarker of gastric cancer in gastric juice. The aim of this study was to develop a novel noninvasive modality for detecting gastric cancer by measurement of α1-antitrypsin concentration in gastric juice. The work consisted of two parts: (a) investigating the differences in gastric juice α1-antitrypsin concentrations between gastric cancer patients and controls, and (b) screening gastric cancer using string test to obtain gastric juice followed by immunoassay for α1-antitrypsin concentration. The data showed that gastric juice α1-antitrypsin concentration was markedly higher in gastric cancer patients than in healthy subjects, gastric ulcer patients, and duodenal ulcer patients (all P < 0.001). The area under the receiver operating characteristic curve for identifying gastric cancer cases was 0.96 (95% confidence interval, 0.93-0.99; P < 0.001). The sensitivity and specificity of gastric juice α1-antitrypsin concentration were 96% and 92%, respectively. Gastric juice α1-antitrypsin assay through string test was validated in 93 consecutive patients for gastric cancer screening. The sensitivity and specificity of gastric juice α1-antitrypsin string test at 85% accuracy were 74% and 88%, respectively. The area under the receiver operating characteristic curve for identifying gastric cancer was 0.84. In conclusion, gastric juice α1-antitrypsin concentration in gastric cancer patients markedly exceeds those in healthy subjects and patients with benign gastrointestinal diseases. A noninvasive α1-antitrypsin string test may serve as a new screening tool for identifying gastric cancer patients. Cancer Epidemiol Biomarkers Prev; 19(2); 405-11. ©2010 AACR.

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Non-endoscopic method to obtain Helicobacter pylori for culture

Cited by 45 publications

References 0 publications

Helicobacter pyloriDetection and Antimicrobial Susceptibility Testing

Helicobacter pyloriDetection and Antimicrobial Susceptibility Testing

α1-Antitrypsin Precursor in Gastric Juice Is a Novel Biomarker for Gastric Cancer and Ulcer

Diagnosis of Gastric Malignancy Using Gastric Juice α1-antitrypsin

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