Non-equilibrium organosilane plasma polymerization for modulating the surface of PTFE towards potential blood contact applications

Abstract

We report a novel and facile organosilane plasma polymerization method designed to improve the surface characteristics of poly(tetrafluoroethylene) (PTFE).

Endothelium-Mimicking Nanomatrix Coating to Enhance Endothelialization after Left Atrial Appendage Closure Device Implantation

“…After incubation, the stained membranes were evaluated by live fluorescence image analysis for comparison. 36 Platelet Adhesion. To assess the coating's effects on platelet adhesion, human whole blood platelets (Innovative Research, MI) were seeded (density, 6 ×10 6 platelets/mL) with uncoated and coated membranes and incubated at 37 °C for 30 min in a 24-well plate.…”

“…Then, each sample was stained with calcein-AM (0.75 μL/mL PBS) and evaluated by live fluorescence image analysis. 36 In Vivo Device Implantation. All protocols were approved by the Institutional Animal Care and Use Committee (IACUC) in compliance with Animal Care regulations.…”

“…After 3 days of incubation at 37 °C, the membranes were stained with calcein-AM (0.75 μL/mL PBS) (Thermo Fisher, NC) and incubated at 37 °C for 20 min. After incubation, the stained membranes were evaluated by live fluorescence image analysis for comparison …”

“…To assess the coating’s effects on platelet adhesion, human whole blood platelets (Innovative Research, MI) were seeded (density, 6 ×10 6 platelets/mL) with uncoated and coated membranes and incubated at 37 °C for 30 min in a 24-well plate. Then, each sample was stained with calcein-AM (0.75 μL/mL PBS) and evaluated by live fluorescence image analysis …”

Heparinized anticoagulant coatings based on polyphenol-amine inspired chemistry for blood-contacting catheters

“…22,23 According to various immobilization methods of heparin, activating inert catheter materials such as polyurethane is a key issue for surface modification. A series of strategies have been performed to enrich the surface with active functional groups such as hydroxyl, amine, carboxyl or sulfhydryl groups, mainly including plasma treatment/polymerization, [24][25][26][27] surface grafting and silanization. [28][29][30][31][32] However, the application of these strategies involving multiple steps or specific devices remains controversial in terms of in vivo biocompatibility or clinical practice.…”