Non-instrumental immunochemical tests for rapid ochratoxin A detection in red wine

Enzyme-Linked Aptamer Assays (ELAAs), based on a competition format for a rapid and sensitive detection of Ochratoxin A in wine

“…In order to determine whether the amount of ethanol (12%) inhibited the aptamereOTA binding, ic-ELAA and dc-ELAA controls were performed using buffer containing respectively 2, 6 and 12% (v/v) of ethanol. The results demonstrated that the percentage of alcohol in wine was not high enough to inhibit the binding between aptamers and OTA (data not shown), as it has been previously shown for the binding between antibody and OTA (Ngundi et al, 2005;Rusanova et al, 2009). Then, a sample pretreatment of wine was performed in order to remove polyphenols and to neutralise the pH.…”

Electrochemical Ochratoxin A Immunosensor System Developed on Sulfonated Polyaniline

“…These changes are evident from the impedance measurements and form the basis of the bioaffinity signals between the OTA antigen and antibody reported in this work. Validation of the immunosensor results will be done by enzyme-linked immunosorbent assay (ELISA) [21,35].…”

Levels of ochratoxins in Mediterranean red wines

“…However, several studies reported high values of OTA contamination from Northern Europe wines, for instance, 4.4 g·L -1 in a wine sample from Russia (Rusanova, Beloglazova, Goryacheva, Lobeau, Van Peteghem & De Saeger, 2009) or the values described at the SCOOP report (2002): 1.90 g·L -1 (Finland); 2,49 g·L -1 (Sweeden) and 3.10 g·L -1 (The Netherlands). These data are much higher than those found in this study in Mediterranean countries.…”