Non-invasive limb ischemic pre-conditioning reduces oxidative stress and attenuates myocardium ischemia-reperfusion injury in diabetic rats

Abstract

This study was to explore whether repeated non-invasive limb ischemic pre-conditioning (NLIP) can confer an equivalent cardioprotection against myocardial ischemia-reperfusion (I/R) injury in acute diabetic rats to the extent of conventional myocardial ischemic pre-conditioning (MIP) and whether or not the delayed protection of NLIP is mediated by reducing myocardial oxidative stress after ischemia-reperfusion. Streptozotocin-induced diabetic rats were randomized to four groups: Sham group, the I/R group, the MIP group and the NLIP group. Compared with the I/R group, both the NLIP and MIP groups showed an amelioration of ventricular arrhythmia, reduced myocardial infarct size, increased activities of total superoxide dismutase (SOD), manganese-SOD and glutathione peroxidase, increased expression of manganese-SOD mRNA and decreased xanthine oxidase activity and malondialdehyde concentration (All p < 0.05 vs I/R group). It is concluded that non-invasive limb ischemic pre-conditioning reduces oxidative stress and attenuates myocardium ischemia-reperfusion injury in diabetic rats.

Interaction of Risk Factors, Comorbidities, and Comedications with Ischemia/Reperfusion Injury and Cardioprotection by Preconditioning, Postconditioning, and Remote Conditioning

“…Zhu et al (2011b) found that the diabetic rat heart in vivo was amenable to infarct size reduction elicited by limb preconditioning (3 Â 5-minute cycles of hindlimb cuff inflation/deflation). However, it is important to note that in this study streptozotocin-induced diabetes was only initiated 1 week before MI.…”

Repeated Non-Invasive Limb Ischemic Preconditioning Confers Cardioprotection Through PKC-Ԑ/STAT3 Signaling in Diabetic Rats

“…However, NLIP is less efficacious in certain pathological states, such as diabetes, senescence and obesity. Interestingly, we showed that application of 3 cycles of NLIP per day for 3 days (repeated NLIP, rNLIP) attenuated myocardial I/RI in diabetic rats [11-13], suggesting that, in order to confer cardioprotection under pathological conditions, the threshold of NLIP should be increased. However, the mechanism whereby rNLIP may confer cardioprotection in diabetes is unclear.…”

“…We previously found that rNLIP conferred cardioprotection in diabetic rats [11] whose cardiac STAT3 was initially reduced [15]. It is possible, therefore, that enhancing cardiac STAT3 activation may be a mechanism to restore rNLIP in diabetes.…”

The Optimal Strategy of Noninvasive Limb Ischemic Preconditioning for Protecting Heart Against Ischemia-Reperfusion Injury in Rats

“…Lujan et al reported that partial hind limb occlusion reduced the susceptibility to sustained ventricular tachycardia in conscious rats [22]. Moreover, Zhu et al found that LIPC can confer similar cardioprotection from myocardial I/R injury in acute diabetic rats to the extent of conventional MIPC [23].…”