Non-invasive sampling for detection of genital infection withChlamydia trachomatis in males utilising urinary leukocyte esterase tests and immunoassays

Domeika, Marius; Bassiri, Mansour; Mårdh, Per‐Anders

doi:10.1007/bf01739005

Cited by 13 publications

(10 citation statements)

References 24 publications

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“…For this reason, noninvasive antigen detection tests, such as DIF (11) and EIA (5,9,14), have been proposed as alternative diagnostic means. Two DNA amplification techniques for the detection of urogenital infections due to C. trachomatis have been developed, i.e., PCR and ligase chain reaction (1,6,12,24).…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of genital Chlamydia trachomatis infections in asymptomatic males by testing urine by PCR

1994

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An enzyme-linked immunosorbent assay (EIA) (MikroTrak; Syva) was compared with PCR (Amplicor; Roche) for detection of Chlamydia trachomatis in first-void urine (FVU) from 184 men attending a skin and venereal disease clinic. The prevalence of C. trachomatis in the population studied was 18.5%. Discrepant results between Syva EIA and Roche PCR were retested by using major outer membrane protein primer-based PCR. After retesting, the sensitivity, the specificity, and the positive and negative predictive values for the Syva EIA were 85.3, 100, 100, and 77.5%, respectively, and those for the Roche PCR 100, 100, 100, and 100%, respectively. It was concluded that PCR provides a highly sensitive and specific noninvasive screening method for genital chlamydial infection in asymptomatic men.

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Section: Discussionmentioning

confidence: 99%

Diagnosis of genital Chlamydia trachomatis infections in asymptomatic males by testing urine by PCR

1994

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“…Historically, these specimens have been successfully tested using cell culture in combination with direct immunofluorescence test (DIF) (1). Later, this diagnostic battery was complemented by enzyme immunoassay (EIA) (2,3), polymerase chain reaction (PCR) (4,5) and ligase chain reaction (LCR) (6,7).…”

mentioning

confidence: 99%

“…Testing of urine by cell culture met problems because of its low sensitivity (8). EIA, which was successfully applied on the urine of males (2,3), however, did not possess sensitivity enough to become a non-invasive sampling approach in females (9). The low sensitivity of EIA probably reflects the limited numbers of microorganisms released from the urethra into female urine.…”

mentioning

confidence: 99%

Evaluation of vaginal introital sampling as an alternative approach for the detection of genital Chlamydia trachomatis infection in women

Domeika¹,

Bassiri²,

Butrimienė³

et al. 1999

Acta Obstet Gynecol Scand

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“…There was overall general acceptance of providing a urine sample. This study reinforced findings by others (Gaydos et al 1998;Domeika 1994) that provision of a urine sample is acceptable by military personnel. The National Guidelines for the Management of STIs (SHSV 2008) in Australia recommend the sample be acquired through first catch urine, of between 10 and 20 mls at least 1 hour after previously passing urine.…”

Section: Urine Samplingsupporting

confidence: 79%

A study of Chlamydia trachomatis: sexual risk behaviour, infection and prevention in the Australian Defence Force

Lambert¹

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There is limited research in Australia focusing on C. trachomatis infection at a population level utilising systematic non-random recruitment methodologies. In addition, little is known in the Australian Defence Force (ADF) about the prevalence of C. trachomatis and of risk behaviours that may impact on sexual health. This study utilised an existing process in the ADF, the Annual Health Assessment, to source new information about C. trachomatis infection and about sexual risk and behaviour. The outcomes of this research may assist the ADF to maintain the health of personnel and consequently a high degree of operational preparedness, and may contribute to the understanding of C. trachomatis prevalence in Australia and across the world.The aims of this study were to determine the prevalence of C. trachomatis infection and to identify potential high risk populations in the ADF with a view to discussing secondary prevention interventions for the control of C. trachomatis infection within the ADF.Seven hundred and thirty-three ADF personnel were recruited into the study over a 24 month period. Participants were asked to complete an 8 page comprehensive survey about sexual behaviour and to provide a urine sample to be tested for C. trachomatis. Ethics approval was received from both the Australian Defence Human Research EthicsCommittee and the University of Queensland Medical Research Ethics Committee.The study examined ADF personnel on deployment and on base in order to provide a comparison of populations across varying work environments. Similarly, this study examined ADF personnel who had recently taken a course of doxycycline for malaria prophylaxis as part of deployment and those who had not in order to assess whether the prophylactic use of antibiotics for other purposes may have had an effect on the acquisition of C. trachomatis. This study enrolled participants from the three services in the ADF, the army, navy and air force, across 10 sites in Australia and four deployments to obtain a wide cross section of the ADF population. The findings from this study provide a profile of ADF sexual health and sexual behaviour that is comparable to the Australian population and identifies, in some instances, greater levels of safe practice and lower levels of risk behaviour. Additionally, the ADF has a lower prevalence of C. trachomatis This study examined rates of attendance at health services for sexual health concerns.18.12% (age and gender adjusted) of participants indicated they attended a health service for a sexual health concern in the previous 12 months. Females were significantly more likely to attend a health service than males (26.45% vs 16.52%; p=0.005; OR 1.81 95% CI 1.19-2.76) for a sexual health matter. Participants were three times more likely to attend an ADF health service for testing and treatment of an STI than to attend a civilian health service (16.4% vs 5.61% p<0.0001). Those who engaged in some unsafe sex were significantly more likely to attend a health service for a sexual health matter ...

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Non-invasive sampling for detection of genital infection withChlamydia trachomatis in males utilising urinary leukocyte esterase tests and immunoassays

Cited by 13 publications

References 24 publications

Diagnosis of genital Chlamydia trachomatis infections in asymptomatic males by testing urine by PCR

Diagnosis of genital Chlamydia trachomatis infections in asymptomatic males by testing urine by PCR

Evaluation of vaginal introital sampling as an alternative approach for the detection of genital Chlamydia trachomatis infection in women

A study of Chlamydia trachomatis: sexual risk behaviour, infection and prevention in the Australian Defence Force

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