Non-ionic Surfactant Modified Cationic Liposomes Mediated Gene Transfection in Vitro and in the Mouse Lung

Ding, Wuxiao; Izumisawa, Tomohiro; Hattori, Yoshiyuki; Qi, Xian-Rong; Kitamoto, Daï; Maitani, Yoshie

doi:10.1248/bpb.32.311

Cited by 19 publications

(7 citation statements)

References 26 publications

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“…The other compounds were identified as quercetin-3,6-dimethyl ether ( 2 ) [18], quercetin-3-methyl ether ( 3 ) [18], quercetin ( 4 ) [18], axillarin-7- O - β - d -glucopyranoside ( 5 ) [19], quercetagetin-3,7-dimethoxy-6- O - β - d -glucopyranoside ( 6 ) [20], quercetagetin-7-methoxy-6- O - β - d -glucopyranoside ( 7 ) [20], and quercetagetin-6- O - β - d -glucopyranoside ( 8 ) [20] by comparison of their physical and spectral data with those in the literature. The antioxidant activity of the isolated compounds 2 - 9 was determined by using a DPPH free radical scavenging system.…”

Section: Resultsmentioning

confidence: 99%

New Thiophene and Flavonoid from Tagetes minuta Leaves Growing in Saudi Arabia

et al. 2014

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Phytochemical investigation of the methanolic extract of Tagetes minuta L. (Asteraceae) leaves resulted in the isolation and identification of two new compounds: 5-methyl-2,2',5',2'',5'',2''',5''',2''''-quinquethiophene (1) and quercetagetin-6-O-(6-Ocaffeoyl-β-D-glucopyranoside) (9), in addition to seven known compounds: quercetin-3,6-dimethyl ether (2), quercetin-3-methyl ether (3), quercetin (4), axillarin-7-O-β-Dglucopyranoside (5), quercetagetin-3,7-dimethoxy-6-O-β-D-glucopyranoside (6), quercetagetin-7-methoxy-6-O-β-D-glucopyranoside (7), and quercetagetin-6-O-β-Dglucopyranoside (8). The compounds were identified by UV, IR, 1D, 2D NMR, and HRESIMS spectral data. They showed significant antioxidant activity, comparable with that of propyl gallate. Compounds 8 and 3 showed weak to moderate antileishmanial and antimalarial activities, with IC 50 values of 31.0 μg/mL and 4.37 μg/mL, respectively.

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Section: Resultsmentioning

confidence: 99%

New Thiophene and Flavonoid from Tagetes minuta Leaves Growing in Saudi Arabia

et al. 2014

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“…Zeta potentials of sample solutions containing MEE and ADBAC were measured using zeta potential and particle size analyzer ELSZ-1000 Otsuka Electronics, Osaka, Japan; measurement accuracy: 0.01 mV 21 .…”

Section: Measurement Of Zeta Potentialmentioning

confidence: 99%

Nonionic Surfactants Enhancing Bactericidal Activity at Their Critical Micelle Concentrations

et al. 2015

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“…It might be due to membrane fusion between plasma membrane and cationic liposomes induced by MEL-A. 12,[14][15][16][17] It is hard to image how MEL-A-modified OH-Chol-lipoplexes exhibited higher cellular association than Tween 80-modified OHChol-lipoplexes, since the former had much lower zeta potential (Fig. 2B); however, the relatively dehydrated surface of MEL-A-modified OH-Chol-lipoplexes (Fig.…”

Section: The Hydration Level Of Liposomes and Lipoplexes As Monitoredmentioning

confidence: 99%

Surface Properties of Lipoplexes Modified with Mannosylerythritol Lipid-A and Tween 80 and Their Cellular Association

Ding

Hattori

et al. 2009

CHEMICAL & PHARMACEUTICAL BULLETIN

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For effective gene transfection into mammalian cells, numerous cationic lipids have been synthesized and formulated into cationic liposomes. [1][2][3] In general, the diverse cationic lipids contain a hydrophobic region, a linker and a cationic headgroup, which are substantially quite variable in different cationic lipids. However, in the cationic headgroup, the basic element is amine, which is responsible for DNA complexation as a protonized form. All four amine types, from primary to quaternary amine, have been reported in diverse cationic lipids and exhibited substantially different cellular association and gene transfection capabilities. 4,5) Understanding the key step in gene transfection, i.e. cellular association of lipoplexes, which is largely determined by the physiochemical properties of cationic liposomes and their lipoplexes, will provide valuable information for designing more effective cationic lipids and optimizing the liposomal formulation. Particle size and zeta potentials of lipoplexes are often reported when discussing their cellular association [6][7][8] ; however, another physiochemical property, the surface hydration of lipoplexes, is always neglected. Although the hydration levels of lipoplexes containing different liposomal formulations have been well established, 9-11) their effect on the cellular association of lipoplexes is still lacking.We reported previously that cationic liposomes, composed of dioleoylphosphatidylethanolamine (DOPE) and N,Nmethyl hydroxyethyl aminopropane carbamoyl cholesterol (MHAPC) or cholesteryl-3b-carboxyamindoethylene-N-hydroxyethylamine (OH-Chol), demonstrated distinctive differences in their cellular association and gene transfection efficiencies in vitro.12) The major differences between MHAPC and OH-Chol are the amine types and linkers shown in Fig. 1, with MHAPC having a tertiary amine linked to the cholesteryl skeleton by a carbamate ester, and OH-Chol having a secondary amine with an amido linker. To understand the different cellular association of lipoplexes composed of different cationic lipids, the effect of amine types and linkers on the surface properties, e.g. zeta potentials and surface hydration levels, was examined. Furthermore, mannosylerythritol lipid-A (MEL-A) is a newly developed biosurfactant 13) and modification of OH-Chol-liposomes with MEL-A markedly increased the gene transfection efficiency of plasmid DNA.14,15) Recent findings found that MEL-A induced membrane fusion between the target cells and cationic liposomes 16,17) ; however, the physiochemical mechanism of the fusion ability of MEL-A has not been fully understood. As a

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Non-ionic Surfactant Modified Cationic Liposomes Mediated Gene Transfection in Vitro and in the Mouse Lung

Cited by 19 publications

References 26 publications

New Thiophene and Flavonoid from Tagetes minuta Leaves Growing in Saudi Arabia

New Thiophene and Flavonoid from Tagetes minuta Leaves Growing in Saudi Arabia

Nonionic Surfactants Enhancing Bactericidal Activity at Their Critical Micelle Concentrations

Surface Properties of Lipoplexes Modified with Mannosylerythritol Lipid-A and Tween 80 and Their Cellular Association

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