Non-particulate (continuous bed or monolithic) acrylate-based capillary columns for reversed-phase liquid chromatography and electrochromatography

Kornyšova, Olga; Maruška, Audrius; Owens, Paul K.; Erickson, Magnus

doi:10.1016/j.chroma.2004.10.078

Cited by 34 publications

(21 citation statements)

References 17 publications

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“…The method was improved by using the alkyl ammonium salt comonomer above its CMC in order to solubilise the additional highly nonpolar monomers. Higher hydrophobicity and peak efficiency were subsequently observed [25]. Recently, Rassi and coworkers [22,23] developed a series of RP/ionexchange mixed-mode acrylate-based monoliths by the copolymerisation of pentaerythritol diacrylate monostearate (PEDAS) with a quaternary ammonium acrylate monomer.…”

Section: Introductionmentioning

confidence: 98%

“…However, due to the incompatible solubility of nonpolar long alkyl chain functional monomers with relatively polar ionexchange functional monomers, the hydrophobicity of most reported one-step copolymerised monoliths was based on short alkyl chain monomers or just crosslinkers. KornyÐova et al [25] prepared long alkyl chain mixed-mode monoliths in an aqueous polymerisation medium by using a water-soluble alkyl ammonium salt comonomer. However, the hydrophobicity of the monolithic phase was very low.…”

Section: Introductionmentioning

confidence: 99%

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Mixed‐mode reversed‐phase and ion‐exchange monolithic columns for micro‐HPLC

Jiang

Smith

Ferguson

et al. 2008

J of Separation Science

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This paper describes the fabrication of RP/ion-exchange mixed-mode monolithic materials for capillary LC. Following deactivation of the capillary surface with 3-(trimethoxysilyl)propyl methacrylate (gamma-MAPS), monoliths were formed by copolymerisation of pentaerythritol diacrylate monostearate (PEDAS), 2-sulphoethyl methacrylate (SEMA) with/without ethylene glycol dimethacrylate (EDMA) within 100 microm id capillaries. In order to investigate the porous properties of the monoliths prepared in our laboratory, mercury intrusion porosimetry, SEM and micro-HPLC were used to measure the monolithic structures. The monolithic columns prepared without EDMA showed bad mechanical stability at high pressure, which is undesirable for micro-HPLC applications. However, it was observed that the small amount (5% w/w) of EDMA clearly improved the mechanical stability of the monoliths. In order to evaluate their application for micro-HPLC, a range of neutral, acidic and basic compounds was separated with these capillaries and satisfactory separations were obtained. In order to further investigate the separation mechanism of these monolithic columns, comparative studies were carried out on the poly(PEDAS-co-SEMA) monolithic column and two other monoliths, poly(PEDAS) and poly(PEDAS-co-2-(methacryloyloxy)ethyl-trimethylammonium methylsulphate (METAM)). As expected, different selectivities were observed for the separation of basic compounds on all three monolithic columns using the same separation conditions. The mobile phase pH also showed clear influence on the retention time of basic compounds. This could be explained by ion-exchange interaction between positively charged analytes and the negatively charged sulphate group.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Mixed‐mode reversed‐phase and ion‐exchange monolithic columns for micro‐HPLC

Jiang

Smith

Ferguson

et al. 2008

J of Separation Science

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“…A short piece (ca. 1 cm, 75 mm id) of polymeric continuous bed RAM capillary column [28] and unshielded RP continuous bed capillary column [29] were used as the SPME (RAM) capillary insert and SPME (RP) capillary insert, respectively. For the determination of theoretical plate heights, hydrodynamic permeability, hydrophobicity and inverse size-exclusion chromatography (ISEC) experiments, long monolithic capillary columns were used: RAM capillary column L T = 14.5 cm,…”

Section: Methodsmentioning

confidence: 99%

“…Polymerization is initiated either thermally or by UV light. Because of the flexibility in monomer choice, monoliths with a variety of surface chemistries were prepared in our group [16][17][18][19][20]. An attractive feature that makes monoliths amenable as chromatographic supports is that no frits are required, since the permeable stationary phase rods are directly synthesized within the column.…”

Section: Introductionmentioning

confidence: 99%

Coupling of solid‐phase microextraction continuous bed (monolithic) capillaries with capillary zone electrophoresis for direct analysis of drugs in biological fluids

et al. 2008

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Hyperlink robust biocompatible solid-phase microextraction (SPME) devices were prepared using continuous bed (monolithic) restricted-access media (RAM) as the SPME capillary insert. The RAM-based SPME approach was able to simultaneously separate proteins from a biological sample, while directly extracting the active components of caffeine, paracetamol and acetylsalicylic acid from the drug NeoCitramonum. The devices were interfaced with a CZE system and fully automated analysis for sample preconcentration, desorption, separation and quantification of analytes was evaluated. Comparative study of in-line coupled SPME-CZE using RAM and RP capillary inserts was carried out. Using an SPME (RAM) insert, the calculated caffeine, paracetamol and acetylsalicylic acid LODs in a bovine plasma sample were 0.3, 0.8 and 1.9 ng/mL, respectively.

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“…KornyÐova et al [17] developed three approaches for a one-step synthesis strategy to prepare poly(N,N-dimethylformamide) for RP-HPLC and CEC. In the first approach, hexyl acrylate comonomer was dissolved together with water-soluble polar comonomers using a non-ionic detergent.…”

Section: Monoliths For Reversed Phase Chromatographymentioning

confidence: 99%

Recent development of monolithic materials as matrices in microcolumn separation systems

Zhu

Zhang²,

Yuan

et al. 2007

J of Separation Science

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This review summarizes the development of monolithic materials, including both organic and inorganic polymers, according mainly to the papers published in the past two years. Due to their good permeability, fast mass transfer, high stability, and their ease of modification, such materials have been widely used in microcolumn separation systems, not only as stationary phases for CEC and capillary HPLC, but also as substances for sample concentration and enzyme reactor. All the research results demonstrate that monolithic materials in microseparation systems can be expected to play an increasingly important role in the analysis of complex samples.

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Non-particulate (continuous bed or monolithic) acrylate-based capillary columns for reversed-phase liquid chromatography and electrochromatography

Cited by 34 publications

References 17 publications

Mixed‐mode reversed‐phase and ion‐exchange monolithic columns for micro‐HPLC

Mixed‐mode reversed‐phase and ion‐exchange monolithic columns for micro‐HPLC

Coupling of solid‐phase microextraction continuous bed (monolithic) capillaries with capillary zone electrophoresis for direct analysis of drugs in biological fluids

Recent development of monolithic materials as matrices in microcolumn separation systems

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