Non-specific fluorescent whitener stains in the rapid recognition of pulmonary dirofilariasis: a report of 20 cases.

Green, L K; Ansari, M. Qasim; Schwartz, Mary R.; Ro, Jae Y.; Alpert, Lesley

doi:10.1136/thx.49.6.590

Cited by 11 publications

(2 citation statements)

References 25 publications

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“…If the parasite has degenerated, it can easily be missed in hematoxylin and eosinstained tissue. Nonspecific fluorescent whitening stain aids in distinguishing the organisms or its fragments within granulomas (Green et al, 1994). Visceral larva migrans (VLM) or systemic toxocariasis is a zoonotic disease with human viscera involved by the migrating larvae of T. canis or T.…”

Section: Helminthic Infectionsmentioning

confidence: 99%

The Pathology of Community-Acquired Pneumonia

Molina

Walker

2002

Community-Acquired Pneumonia

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Section: Helminthic Infectionsmentioning

confidence: 99%

The Pathology of Community-Acquired Pneumonia

Molina

Walker

2002

Community-Acquired Pneumonia

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“…Principally, CFW is composed of compounds which has the binding ability to chitin and cellulose on the microorganism’s structures [25]. In parasitological field, CFW is known as an optical fluorescence brightener widely used in the rapid detection for spores of microsporidia [26], cysts of Entamoeba [27], cysts and trophozoites of Giardia lamblia [28], cysts of Pneumocystis carinii [29], eggs of Ascaris lumbricoides [30], larvae of Dirofilaria immitis [31] and cysts of Acanthamoeba [32]. In this study, Acanthamoeba in the specimen appeared mainly the cyst form due to the prolonged infection without therapeutic responses.…”

Section: Discussionmentioning

confidence: 99%

The First Acanthamoeba keratitis Case of Non-Contact Lens Wearer with HIV Infection in Thailand

et al. 2019

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Acanthamoeba keratitis (AK) is a rare sight-threatening corneal infection, often reporting from contact lens wearers. An asymptomatic human immunodeficiency virus (HIV)-infected Thai male without history of contact lens use complained foreign body sensation at his left eye during motorbike riding. He had neither specific keratitis symptoms nor common drugs responding, which contributed to delayed diagnosis. By corneal re-scraping, Acanthamoeba-like cysts were detected by calcofluor white staining and agar culture. The etiological agent obtained from the culture was molecularly confirmed by Acanthamoeba spp.-specific PCR, followed by DNA sequencing. The results from BLAST and phylogenetic analysis based on the DNA sequences, revealed that the pathogen was Acanthamoeba T4, the major genotype most frequently reported from clinical isolates. The infection was successfully treated with polyhexamethylene biguanide resulting in corneal scar. This appears the first reported AK case from a non-contact lens wearer with HIV infection in Thailand. Although AK is sporadic in developing countries, a role of free-living Acanthamoeba as an opportunistic pathogen should not be neglected. The report would increase awareness of AK, especially in the case presenting unspecific keratitis symptoms without clinical response to empirical antimicrobial therapy.

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Parasitic Diseases

Seaton¹

2000

Crofton and Douglas's Respiratory Diseases

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Non-specific fluorescent whitener stains in the rapid recognition of pulmonary dirofilariasis: a report of 20 cases.

Cited by 11 publications

References 25 publications

The Pathology of Community-Acquired Pneumonia

The Pathology of Community-Acquired Pneumonia

The First Acanthamoeba keratitis Case of Non-Contact Lens Wearer with HIV Infection in Thailand

Parasitic Diseases

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