Non-thermal, pulsed electric field cell ablation: A novel tool for regenerative medicine and scarless skin regeneration

Golberg, Alexander; Broelsch, G. Felix; Bohr, Stefan; Mihm, Martín C.; Austen, William G.; Albadawi, Hassan; Watkins, Michael T.; Yarmush, Martin L.

doi:10.1142/s233954781320001x

Cited by 38 publications

(41 citation statements)

References 14 publications

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“…In NTIRE, externally applied pulsed electric fields cause irreversible damage to cells by affecting the cell membrane, while sparing the tissue scaffold, large blood vessels, and other tissue structures (83)(84)(85). The preservation of tissue architecture is an important and unique property of NTIRE ablation, and it probably contributes to the reduced scarring observed (as reported in 83,84,86).…”

Section: Nonthermal Tissue Ablationmentioning

confidence: 94%

Electroporation-Based Technologies for Medicine: Principles, Applications, and Challenges

Yarmush

Golberg

Serša³

et al. 2014

Annu. Rev. Biomed. Eng.

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725

443

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When high-amplitude, short-duration pulsed electric fields are applied to cells and tissues, the permeability of the cell membranes and tissue is increased. This increase in permeability is currently explained by the temporary appearance of aqueous pores within the cell membrane, a phenomenon termed electroporation. During the past four decades, advances in fundamental and experimental electroporation research have allowed for the translation of electroporation-based technologies to the clinic. In this review, we describe the theory and current applications of electroporation in medicine and then discuss current challenges in electroporation research and barriers to a more extensive spread of these clinical applications.

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Section: Nonthermal Tissue Ablationmentioning

confidence: 94%

Electroporation-Based Technologies for Medicine: Principles, Applications, and Challenges

Yarmush

Golberg

Serša³

et al. 2014

Annu. Rev. Biomed. Eng.

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725

443

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“…To address the effects of PEF on nontarget tissue, we previously investigated the healing process of normal rat skin ablated by PEF. 38 In this study, we showed that PEF-ablated normal skin regenerated rapidly without scars. 38 Our results show that PEFs preserve the extracellular matrix and the microvasculature of the ablated area—properties that most likely promote scarless skin regeneration.…”

Section: Discussionmentioning

confidence: 57%

“…38 In this study, we showed that PEF-ablated normal skin regenerated rapidly without scars. 38 Our results show that PEFs preserve the extracellular matrix and the microvasculature of the ablated area—properties that most likely promote scarless skin regeneration. 38 …”

Section: Discussionmentioning

confidence: 57%

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Pulsed Electric Fields for Burn Wound Disinfection in a Murine Model

Golberg

Broelsch

Vecchio

et al. 2015

Journal of Burn Care & Research

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Emerging bacterial resistance renders many antibiotics ineffective, making alternative strategies of wound disinfection important. Here the authors report on a new, physical burn wound disinfection method: pulsed electric fields (PEFs). High voltage, short PEFs create nonthermal, permanent damage to cell membranes, possibly by irreversible electroporation. In medicine, PEF technology has recently been used for nonthermal ablation of solid tumors. The authors have expanded the spectrum of PEF applications in medicine to burn wound disinfection. A third-degree burn was induced on the dorsal skin of C57BL/6 mice. Immediately after the injury, the burn wound was infected with Acinetobacter baumannii expressing the luxCDABE operon. Thirty minutes after infection, the infected areas were treated with 80 pulses delivered at 500 V/mm, 70 μs, 1 Hz. The authors used bioluminescence to quantify bacteria on skin. Three animals were used for each experimental condition. PEFs were effective in the disinfection of infected burned murine skin. The bacterial load reduction correlated with the number of delivered pulses. Forty pulses of 500 V/mm led to a 2.04 ± 0.29 Log10 reduction in bacterial load; 80 pulses led to the immediate 5.53 ± 0.30 Log10 reduction. Three hours after PEF, the bacterial reduction of the skin treated with 500 V/mm, 80 pulses was 4.91 ± 0.71 Log10. The authors introduce a new method of wound disinfection using high voltage, short PEFs. They believe that PEF technology may represent an important alternative to antibiotics in addressing bacterial contamination of wounds, particularly those contaminated with multidrug-resistant bacteria.

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“…These limitations include: a significant impact of all factors (listed in Section 1 -such as undisclosed diseases, hot meals consumed before the test, lack of acclimatisation to the room etc. [44][45][46][47][48][49][50]) -on temperature distribution. All these factors can fully disqualify the measurement.…”

Section: Critical Summarymentioning

confidence: 99%

Automatic analysis of the trunk thermal images from healthy subjects and patients with faulty posture

Koprowski

2015

Computers in Biology and Medicine

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Non-thermal, pulsed electric field cell ablation: A novel tool for regenerative medicine and scarless skin regeneration

Cited by 38 publications

References 14 publications

Electroporation-Based Technologies for Medicine: Principles, Applications, and Challenges

Electroporation-Based Technologies for Medicine: Principles, Applications, and Challenges

Pulsed Electric Fields for Burn Wound Disinfection in a Murine Model

Automatic analysis of the trunk thermal images from healthy subjects and patients with faulty posture

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