“…Hydrodynamic injection of IL-15 expressing plasmid (pLIVE-IL-15) induced sustained high expression of IL-15 in serum and liver of mice, and broke HBV-induced immunotolerance (Yin et al, 2012 ). In addition, many other secreted proteins have been introduced in liver by this effective method, for determination of their functions and evaluation of their therapeutic activities, including IL-10 (Hong et al, 2003 ), IL-21 (Brady et al, 2004 ), hepatocyte growth factor (Yang et al, 2001 ), human growth hormone (Sondergaard et al, 2003 ), human factor IX (Miao et al, 2001 ; Ye et al, 2003 ), activin (Chabicovsky et al, 2003 ), human alpha-1 antitrypsin (Zhang et al, 2000 ), Erythropoietin receptor-IgG1Fc fusion protein (Maruyama et al, 2004 ).…”