Non Viral Vectors in Gene Therapy- An Overview

Ramamoorth, Murali; Narvekar, Aparna

doi:10.7860/jcdr/2015/10443.5394

Cited by 501 publications

(518 citation statements)

References 32 publications

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“…Compared to viral-vectors, nonviral vectors have exhibited the potential to break through several limitations, due to their low immune response, high gene-loading capacity, handy chemical modification, large-scale production, and especially, their safety. 12 PEI was one of the most effective nonviral gene carrier agents due to its strong DNA compaction capacity with an intrinsic endosomolytic activity. 24,25 Thus, many PEI-based vectors have been developed.…”

Section: Discussionmentioning

confidence: 99%

Efficient inhibition of ovarian cancer by degradable nanoparticle-delivered survivin T34A gene

Luo

Zhao

et al. 2016

IJN

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Gene therapy has promising applications in ovarian cancer therapy. Blocking the function of the survivin protein could lead to the growth inhibition of cancer cells. Herein, we used degradable heparin–polyethyleneimine (HPEI) nanoparticles to deliver a dominant-negative human survivin T34A (hs- T34A ) gene to treat ovarian cancer. HPEI nanoparticles were characterized and were found to have a dynamic diameter of 66±4.5 nm and a zeta potential of 27.1±1.87 mV. The constructed hs- T34A gene expression plasmid could be effectively delivered into SKOV3 ovarian carcinoma cells by HPEI nanoparticles with low cytotoxicity. Intraperitoneal administration of HPEI/hs- T34A complexes could markedly inhibit tumor growth in a mouse xenograft model of SKOV3 human ovarian cancer. Moreover, according to our results, apparent apoptosis of cancer cells was observed both in vitro and in vivo. Taken together, the prepared HPEI/hs- T34A formulation showed potential applications in ovarian cancer gene therapy.

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Section: Discussionmentioning

confidence: 99%

Efficient inhibition of ovarian cancer by degradable nanoparticle-delivered survivin T34A gene

Luo

Zhao

et al. 2016

IJN

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“…[11] There is a hypothesis that mechanical massage of liver produces transient defects in cell membrane facilitating the delivery of plasmid DNA into hepatic cells by diffusion. [24] Its limitations are included low levels of transfection and transgene expression due to the rapid degradation by nucleases in the serum, and the clearance by the mononuclear phagocyte system. [25] 2.2 Non-viral chemical methods Chemical non-viral methods have been known as an important delivery system designed as natural or synthetic compounds such as polymers, lipids, peptides, and inorganic methods as mentioned in next sections.…”

Section: Non-viral Physical Methodsmentioning

confidence: 99%

Different strategies of gene delivery for treatment of cancer and other disorders

Agi

Mosaferi

Khatamsaz

et al. 2016

JST

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Gene therapy is the gene transfer into host cells for treatment of acquired and genetic disorders. For this purpose, there are a wide variety of gene delivery methods with special properties including viral and non-viral vectors. The non-viral methods use physical forces or chemical compounds (natural or synthetic) to transfer DNA into a cell. The efficiency of the non-viral gene therapy depends on conquering four different intra-and extra-cellular barriers such as cellular uptake, endosomal escape, nuclear entry, and gene expression. Among various gene carriers, some viral vectors such as Adenovirus, Lentivirus, Vaccinia as well as gene gun and lipofection achieved to clinical trials. In this mini-review, we briefly describe different approaches for gene delivery and their applications in various phases of clinical trials.

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“…Recently, non-viral gene vectors such as nanomaterials of lipoplexes, liposomes and polyplexes have also been used for nanosized diagnostic and therapeutic DNA-based nanomedicine approaches [1]. The penetration of the dye into the hair follicles have been investigated in vitro on porcine skin which is an appropriate model for human tissue and it was found that the nanoparticles penetrate much deeper into the hair follicles than any non-particle form [2].…”

Section: Introductionmentioning

confidence: 99%

Biomedical Applications of Silver and Gold Nanoparticles: Effective and Safe Non-Viral Delivery Vehicles

Rani¹

2017

JABB

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Non Viral Vectors in Gene Therapy- An Overview

Cited by 501 publications

References 32 publications

Efficient inhibition of ovarian cancer by degradable nanoparticle-delivered survivin T34A gene

Efficient inhibition of ovarian cancer by degradable nanoparticle-delivered survivin T34A gene

Different strategies of gene delivery for treatment of cancer and other disorders

Biomedical Applications of Silver and Gold Nanoparticles: Effective and Safe Non-Viral Delivery Vehicles

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