Nonactivated versus Thrombin-Activated Platelets on Wound Healing and Fibroblast-to-Myofibroblast Differentiation In Vivo and In Vitro

Scherer, Saja; Tobalem, Mickaël; Vigato, Enrico; Heit, Yvonne I.; M, Ali; Hinz, Boris; Pittet, Brigitte; Pietramaggiori, Giorgio

doi:10.1097/prs.0b013e3182362010

Cited by 93 publications

(78 citation statements)

References 22 publications

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“…57 There is also evidence that PLTs without preactivation stimulate wound healing more efficiently than preactivated PLTs. 58 Indeed, positive clinical results in knee OA have been reported in PRP injections both with and without PLT preactivation. 25,40,44,50,51,59 To date, there are no clinical trials comparing different types of PRP activation.…”

Section: Plt Preactivationmentioning

confidence: 97%

Intraarticular Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis

Pourcho

Smith

Wisniewski

et al. 2014

American Journal of Physical Medicine &Amp; Rehabilitation

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Intraarticular platelet-rich plasma (PRP) injection has emerged as a promising treatment for knee osteoarthritis. Studies to date, including multiple randomized controlled trials, have shown that PRP is a safe and effective treatment option for knee osteoarthritis. Intraarticular PRP is similar in efficacy to hyaluronic acid, and seems to be more effective than hyaluronic acid in younger, active patients with low-grade osteoarthritis. Treatment benefits seem to wane after 6-9 mos. There are numerous PRP treatment variables that may be of importance, and the optimal PRP protocol remains unclear. Future investigations should control and analyze the effects of these variables in PRP treatment. High-quality randomized controlled trials are needed to optimize PRP treatment methods and better define the role of PRP in osteoarthritis management in the knee and, potentially, in other joints.

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Section: Plt Preactivationmentioning

confidence: 97%

Intraarticular Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis

Pourcho

Smith

Wisniewski

et al. 2014

American Journal of Physical Medicine &Amp; Rehabilitation

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“…However, rendering mice thrombocytopenic leads to increased wound inflammation without affecting the reparative aspects of wound repair (56). A recent study demonstrated better healing responses in diabetic mice injected with nonactivated versus thrombin-activated platelets; the authors concluded that platelets may be used as cell therapy rather than exploited for their growth factor content (57). The approach that we have taken preserved the cells and ablated the defined spectrum of growth factors specifically contained in platelet α-granules.…”

Section: Figurementioning

confidence: 99%

Gray platelet syndrome and defective thrombo-inflammation in Nbeal2-deficient mice

Deppermann¹,

Cherpokova²,

Nurden³

et al. 2013

J. Clin. Invest.

166

159

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Platelets are anuclear organelle-rich cell fragments derived from bone marrow megakaryocytes (MKs) that safeguard vascular integrity. The major platelet organelles, α-granules, release proteins that participate in thrombus formation and hemostasis. Proteins stored in α-granules are also thought to play a role in inflammation and wound healing, but their functional significance in vivo is unknown. Mutations in NBEAL2 have been linked to gray platelet syndrome (GPS), a rare bleeding disorder characterized by macrothrombocytopenia, with platelets lacking α-granules. Here we show that Nbeal2-knockout mice display the characteristics of human GPS, with defective α-granule biogenesis in MKs and their absence from platelets. Nbeal2 deficiency did not affect MK differentiation and proplatelet formation in vitro or platelet life span in vivo. Nbeal2-deficient platelets displayed impaired adhesion, aggregation, and coagulant activity ex vivo that translated into defective arterial thrombus formation and protection from thrombo-inflammatory brain infarction following focal cerebral ischemia. In a model of excisional skin wound repair, Nbeal2-deficient mice exhibited impaired development of functional granulation tissue due to severely reduced differentiation of myofibroblasts in the absence of α-granule secretion. This study demonstrates that platelet α-granule constituents are critically required not only for hemostasis but also thrombosis, acute thrombo-inflammatory disease states, and tissue reconstitution after injury.

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“…Numerous wound dressings are in use. Topical use of autologous platelets has also been described [5]. Majority of clinical studies focus on defects classified as Wagner/Armstrong stadium 1 and 2.…”

Section: Introductionmentioning

confidence: 99%

Vascular Surgery, Microsurgery and Supramicrosurgery for Treatment of Chronic Diabetic Foot Ulcers to Prevent Amputations

2013

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IntroductionDiabetic foot ulcers occur in approximately 2,5% of patients suffering from diabetes and may lead to major infections and amputation. Such ulcers are responsible for a prolonged period of hospitalization and co- morbidities caused by infected diabetic foot ulcers. Small, superficial ulcers can be treated by special conservative means. However, exposed bones or tendons require surgical intervention in order to prevent osteomyelitis. In many cases reconstructive surgery is necessary, sometimes in combination with revascularization of the foot.There are studies on non surgical treatment of the diabetic foot ulcer. Most of them include patients, classified Wagner 1-2 without infection. Patients presenting Wagner 3D and 4D however are at a higher risk of amputation. The evolution of microsurgery has extended the possibilities of limb salvage. Perforator based flaps can minimize the donorsite morbidity.Patients and Methods41 patients were treated with free tissue transfer for diabetic foot syndrome and chronic defects. 44 microvascular flaps were needed. The average age of patients was 64.3 years. 18 patients needed revascularization. 3 patients needed 2 microvascular flaps. In 6 cases supramicrosurgical technique was used.ResultsThere were 2 flap losses leading to amputation. 4 other patients required amputation within 6 months postoperatively due to severe infection or bypass failure. Another 4 patients died within one year after reconstruction. The remaining patients were ambulated.DiscussionLarge defects of the foot can be treated by free microvascular myocutaneous or fasciocutaneous tissue transfer. If however, small defects, exposing bones or tendons, are not eligible for local flaps, small free microvascular flaps can be applied. These flaps cause a very low donor site morbidity. Arterialized venous flaps are another option for defect closure.Amputation means reduction of quality of life and can lead to an increased mortality postoperatively.

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Nonactivated versus Thrombin-Activated Platelets on Wound Healing and Fibroblast-to-Myofibroblast Differentiation In Vivo and In Vitro

Abstract: Platelets stimulate wound healing more efficiently compared with activated platelets by enhancing fibroblast differentiation and contractile function. Similar levels of growth factors may induce different biological effects when delivered "on demand" rather than in an initial bolus.

Cited by 93 publications

References 22 publications

Intraarticular Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis

Intraarticular Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis

Gray platelet syndrome and defective thrombo-inflammation in Nbeal2-deficient mice

Vascular Surgery, Microsurgery and Supramicrosurgery for Treatment of Chronic Diabetic Foot Ulcers to Prevent Amputations

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