Noncoding RNAs: New Players in Cancers

Chen, Xueman; Fan, Shengnuo; Song, Erwei

doi:10.1007/978-981-10-1498-7_1

Cited by 65 publications

(47 citation statements)

References 349 publications

(239 reference statements)

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“…Recently, increasing evidence confirms that long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) have been identified as important regulators in a variety of cancers including HCC (Bartel, 2004;Li and Chang, 2014;Wang et al, 2017). Abnormal expression of lncRNA plays a key role in cancer progression and carcinogenesis through a variety of mechanisms (Chen et al, 2016). LINC00346 is located on chromosome 13q34, with a total length of 6322bp, and is up-regulated and has oncogenic effects in non-small cell lung cancer and bladder cancer (Ye et al, 2017;.…”

Section: Introductionmentioning

confidence: 99%

LINC00346 Acts as a Competing Endogenous RNA Regulating Development of Hepatocellular Carcinoma via Modulating CDK1/CCNB1 Axis

Jin

et al. 2020

Front. Bioeng. Biotechnol.

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Hepatocellular carcinoma (HCC) is one of the important types of liver cancer. LncRNA is an important regulatory factor that regulates many biological processes such as tumor cells during tumorigenesis and metastasis. LINC00346 has been associated with various types of liver cancer, but its role and regulatory mechanism in HCC remain unclear. In our study, we found the LINC00356-miR-199a-3p-CDK1/CCNB1 axis through bioinformatics analysis. The expressions of LINC00356, miR-199a-3p, CDK1, and CCNB1 in HCC and normal hepatocytes were determined by qRT-PCR and WB. The results showed that LINC00356, CDK1 and CCNB1 were highly expressed in HCC, while miR-199a-3p was lowly expressed. Dual luciferase reporter gene assay, RIP and RNA-pull down assays demonstrated the targeted binding relationship of LINC00346-miR-199a-3p-CDK1/CCNB1. Overexpressing LINC00460 and silencing miR-199a-3p promoted cell invasion, inhibited apoptosis of HCC, and arrested the cell cycle in S phase while opposite results were obtained when silencing LINC00346, CDK1, and CCNB1. LINC00346 indirectly affects liver cancer by promoting the expression of CDK1/CCNB1 through competitive adsorption of miR-199a-3p. In addition, the study also demonstrated that overexpression of LINC00346 indirectly inhibited the expression of p53 and p21 proteins by promoting CDK1/CCNB1 expressions, thereby blocking the p53 signaling pathway. These results proved that LINC00346 could regulate the expression of CDK1/CCNB1 through the competitive adsorption of miR-199a-3p, thereby affecting the p53 signaling pathway and finally regulating the apoptosis, invasion and cell cycle of HCC cells. In conclusion, LINC00346 can be used as a tumor promoter and potential therapeutic target for HCC metastasis and prognosis.

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Section: Introductionmentioning

confidence: 99%

LINC00346 Acts as a Competing Endogenous RNA Regulating Development of Hepatocellular Carcinoma via Modulating CDK1/CCNB1 Axis

Jin

et al. 2020

Front. Bioeng. Biotechnol.

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“…Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) are two kinds of non-coding RNAs, which are not able to encode any protein [4]. The existing researches have showed that the abnormally expressed lncRNAs, miRNAs and proteins regulate the EMT progression of HCC cells via their interactions [5–7].…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA CASC2 suppresses epithelial-mesenchymal transition of hepatocellular carcinoma cells through CASC2/miR-367/FBXW7 axis

et al. 2017

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BackgroundRecently, it has been reported that long non-coding RNA (lncRNA) cancer susceptibility candidate 2 (CASC2), a novel tumor suppressor, participates in regulating the carcinogenesis and suppresses tumor progression by sponging microRNAs (miRNAs). However, the expression and function of CASC2 in hepatocellular carcinoma (HCC) remain unclear.MethodsThe expression of CASC2 and miR-367 in HCC specimens and cell lines were detected by real-time PCR. Western blotting and immunohistochemistry were carried out for detection of epithelial-to-mesenchymal transition (EMT) markers in HCC. Transwell assays were used to determine migration and invasion of HCC cells. A mouse model for lung metastasis was established to evaluated HCC metastasis in vivo. The correlation among CASC2, miR-367 and F-box and WD repeat domain containing 7 (FBXW7) were disclosed by a dual-luciferase reporter assay, RIP assay and biotin pull-down assay.ResultsHere, CASC2 expression was significantly downregulated in HCC tissues, especially in aggressive and recurrent cases. In accordance, CASC2 underexpression was observed in HCC cell lines compared to LO2. In vitro and in vivo experiments revealed that CASC2 inhibited migration and invasion of HCC cells. Additionally, CASC2 repressed EMT process of HCC cells. Further studies demonstrated that CASC2 could function as a competing endogenous RNA (ceRNA) by sponging miR-367 in HCC cells. Functionally, gain- and loss-of-function studies showed that miR-367 promoted migration, invasion and EMT progression of HCC cells. Moreover, further investigations disclosed that FBXW7 was a downstream target of miR-367 and CASC2 prohibited EMT progression and subsequently exerted its anti-metastatic effects via CASC2/miR-367/FBXW7 axis in HCC cells. Clinically, CASC2 underexpression and miR-367 overexpression were closely correlated with the metastasis-associated clinicopathologic features. Notably, CASC2 low-expressing and miR-367 high-expressing HCC patients showed the poorest clinical outcome.ConclusionsOverall, we conclude that the CASC2/miR-367/FBXW7 axis may be a ponderable and promising therapeutic target for HCC.Electronic supplementary materialThe online version of this article (doi:10.1186/s12943-017-0702-z) contains supplementary material, which is available to authorized users.

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“…Noncoding RNAs (ncRNAs) are defined as RNAs possessing little capability of protein‐coding. They function as promising regulators of epigenetic, transcriptional, and posttranscriptional gene expression . Generally, based on the lengths, ncRNAs are categorized into small ncRNAs (shorter than 200 nucleotides, like microRNAs [miRNAs], small interfering RNAs [siRNAs], and transfer RNAs [tRNAs]) and long ncRNAs (lncRNAs) (longer than 200 nucleotides, like lincRNAs and macroRNAs) .…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA GAS5 does not regulate HBV replication

Feng

et al. 2019

Journal of Medical Virology

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Hepatitis B virus (HBV) infection remains a severe health burden worldwide.Emerging long noncoding RNAs (lncRNAs) are hijacked to enhance virus replication or employed by the host to stimulate immune responses to clear the virus. LncRNA growth arrest-specific transcript 5 (GAS5) can regulate RNA virus by suppressing the replication of both hepatitis C virus and human immunodeficiency virus. In this study, we explored the changes of HBV replication by overexpressing or knocking down GAS5 in HepAD38 cell and HepG2 cell transfected with pHBV1.2. We found HBV can induce the expression of GAS5. However, GAS5 had no effect on extracellular HBsAg and HBeAg, nor intracellular HBV RNA and HBV DNA. In addition, GAS5 possessed similar expression levels between stable HBV-producing cell lines and hepatoma cell lines. Furthermore, GAS5 showed no difference between healthy subjects and patients with chronic HBV in multiple GEO microarray data sets by GEO2R analysis.Taken together these results, GAS5 does not modulate the replication of HBV but it inhibits cell proliferation in HepAD38. This provides insights into the possible roles of GAS5 in HBV infection. K E Y W O R D Sbioinformatics, growth arrest-specific transcript 5, hepatitis B virus, replication

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Noncoding RNAs: New Players in Cancers

Cited by 65 publications

References 349 publications

LINC00346 Acts as a Competing Endogenous RNA Regulating Development of Hepatocellular Carcinoma via Modulating CDK1/CCNB1 Axis

LINC00346 Acts as a Competing Endogenous RNA Regulating Development of Hepatocellular Carcinoma via Modulating CDK1/CCNB1 Axis

Long non-coding RNA CASC2 suppresses epithelial-mesenchymal transition of hepatocellular carcinoma cells through CASC2/miR-367/FBXW7 axis

Long noncoding RNA GAS5 does not regulate HBV replication

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