“…An easily available instrument, the chlorophyll meter (eg SPAD-502 chlorophyll meter), is used widely to evaluate the plant chlorophyll content and, hence, to estimate the plant N status and deficiencies and, subsequently, to determine the real-time based need for N fertiliser with introducing a proper calibration (Jifon et al, 2005). Experimental relationships between N concentration and SPAD reading values are expressed with linear (Chang and Robison, 2003) or curvilinear (Dwyer et al, 1995) models depending on the crops, the species, the cultivars and the crop growth stage (Esfahani et al, 2008). In addition, any biotic (eg pests, diseases, weeds) and abiotic stresses (eg drought, heat, cold, salinity) may influence the SPAD reading values and, subsequently, the chlorophyll or N estimates (Balasubramanian et al, 2000).…”