Nonintegrin laminin receptor precursor protein is expressed on olfactory stem and progenitor cells

Abstract

The biochemical identification and immunocytochemical characterization of a cell surface antigen, expressed on globose basal cells (GBCs) of the rodent olfactory epithelium (OE), are described. The monoclonal antibody (MAb) GBC-3 recognizes a surface protein, confirmed by both live cell staining and fluorescence-activated cell sorting. Two-dimensional SDS-PAGE/Western blot followed by tandem mass spectrometry demonstrates that the cell surface GBC-3 antigen is the 40 kDa laminin receptor precursor protein. The MAb GBC-3 labels the vast majority of cells among the GBC population and does not stain either sustentacular cells or horizontal basal cells (HBCs) in the normal rat OE. After epithelial lesion by exposure to methyl bromide, the remaining cells, which are mostly GBCs, are heavily stained by GBC-3, and colabeled with GBC-3 and sustentacular cell or HBC markers. GBC-3 will be a potentially useful tool for identifying and characterizing GBCs. Indexing terms2D IEF/SDS-PAGE; tandem mass spectrometry; cell surface marker; nonintegrin laminin receptors; tissue stem cells; progenitors The capacity of the olfactory epithelium (OE) for sustained neurogenesis and to recover after injury implies that the adult OE retains a population of tissue stem cells. Based in part on proliferative capacity, basal cells-globose basal cells (GBCs) and horizontal basal cells (HBCs)-have been deemed likely candidates for olfactory stem cells. While still subject to debate, there are ample data to support the notion that among the GBCs are putative stem cells with the capacity to give rise to both neurons and nonneuronal cells during reconstitution of the OE after various types of injury. That GBCs are neuronal progenitors has been well established by studies in unlesioned OE or following bulbectomy including; 1) pulse-chase experiments using 3 H-thymidine (Graziadei, 1973;Graziadei and Monti Graziadei, 1979); 2) increased proliferation of GBCs, but not HBCs, during the accelerated neurogenesis following bulbectomy (Schwartz Levey et al., 1991); and 3) lineage tracing using replication-incompetent, retroviral vectors (Caggiano et al., 1994;Schwob et al., 1994;Huard et al., 1998). However, a broader-than-neuronal potency (that is, multipotency) of NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author ManuscriptGBCs has been demonstrated in other settings; for example, following MeBr lesion when multiple cell types are destroyed and need replacing. The most direct evidence of broad multipotency derives from experiments using transplantation of FACS-purified cell types into the MeBr-lesioned OE, which is a type of colony-forming unit (CFU) assay in vivo. In aggregate, FACS-sorted GBCs harvested from normal epithelium produce colonies containing olfactory sensory neurons (OSN), sustentacular cells, GBCs, Bowman's gland/ duct (BG/D) cells, and respiratory columnar epithelial cells following transplantation into the MeBr-lesioned epithelium (Chen et al., 2004). Moreover, a subset of GBCs in the normal epithelium has the ...

An Epigenetic Signature for Monoallelic Olfactory Receptor Expression

“…We also examined the chromatin state of OR genes in other progenitor cells from the MOE that are negative for OMP, ICAM-1, iLR (immature laminin receptor) (another marker for multipotent basal cells (Jang et al, 2007)) and SUS4. As seen in Figure 4G, in this population the enrichment for H3K9me3 and H4K20me3 on the tested ORs is as high as in the OMP + cells.…”

Establishment of a protocol for obtention of neuronal stem cells lineages from the dog olfactory epithelium

“…It has been reported that mammal olfactory epithelium (OE) consisted of olfactory receptor neurons, supporting cells, global and horizontal basal stem cells, which have been demonstrated to be responsible for replacing damaged cells (Chen et al 2004, Jang et al 2007). Therefore, OE has been considered to be an essential source for adult neural progenitor cells.…”

Morphological assessment of the luminal surface of olfactory epithelium in mice deficient in tissue plasminogen activator following bulbectomy

“…Non-integrin laminin receptor precursor protein is expressed in the olfactory stem and progenitor cells. 14 are expressed in the olfactory epithelium, 15,16 and their expression increases in the olfactory epithelium 15,17 and olfactory bulb. 18 Plasmin may play a role in degrading laminin and activating matrix metalloproteinases.…”