Noninvasive Cell-Based Impedance Spectroscopy for Real-Time Probing Inhibitory Effects of Graphene Derivatives

Male, Keith B.; Lam, Edmond; Montes, Johnny; Luong, John H. T.

doi:10.1021/am301060z

Cited by 10 publications

(5 citation statements)

References 44 publications

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“…On the other hand, podocytes are highly polarized cells, characterized by a dynamic actin-based cytoskeleton and small actin-based foot processes that can be rapidly remodeled upon exposure to toxins . These known differences can explain the steeper decrease in cell barrier resistance detected in the case of podocytes after 24 h. Previous studies that evaluated cytotoxic responses to graphene-based material exposure by ECIS using insect cells and HeLa cells report an adequate cell adherence indicative of biocompatibility. To the best of our knowledge, the use of ECIS to determine the barrier function of human kidney cells in response to graphene-based materials has not been previously reported.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Extensive Glomerular Filtration of Thin Graphene Oxide Sheets on Kidney Physiology

Jasim¹,

Murphy²,

Newman³

et al. 2016

ACS Nano

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Understanding how two-dimensional (2D) nanomaterials interact with the biological milieu is fundamental for their development toward biomedical applications. When thin, individualized graphene oxide (GO) sheets were administered intravenously in mice, extensive urinary excretion was observed, indicating rapid transit across the glomerular filtration barrier (GFB). A detailed analysis of kidney function, histopathology, and ultrastructure was performed, along with the in vitro responses of two highly specialized GFB cells (glomerular endothelial cells and podocytes) following exposure to GO. We investigated whether these cells preserved their unique barrier function at doses 100 times greater than the dose expected to reach the GFB in vivo. Both serum and urine analyses revealed that there was no impairment of kidney function up to 1 month after injection of GO at escalating doses. Histological examination suggested no damage to the glomerular and tubular regions of the kidneys. Ultrastructural analysis by transmission electron microscopy showed absence of damage, with no change in the size of podocyte slits, endothelial cell fenestra, or the glomerular basement membrane width. The endothelial and podocyte cell cultures regained their full barrier function after >48 h of GO exposure, and cellular uptake was significant in both cell types after 24 h. This study provided a previously unreported understanding of the interaction between thin GO sheets with different components of the GFB in vitro and in vivo to highlight that the glomerular excretion of significant amounts of GO did not induce any signs of acute nephrotoxicity or glomerular barrier dysfunction.

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Section: Discussionmentioning

confidence: 99%

The Effects of Extensive Glomerular Filtration of Thin Graphene Oxide Sheets on Kidney Physiology

Jasim¹,

Murphy²,

Newman³

et al. 2016

ACS Nano

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“…5 suggest considerable values of the binding energy, which are consistent with the outcomes of H. Quan et al, 74 since they reported that the presence of strong electron-withdrawing groups on benzene sulfonyl 5-FU greatly enhanced the binding selectivity. 75,76 To perform accurate calculations of the transmission coefficient for the molecular junctions with sulfonyl-anchor groups, the binding energies were computed using eqn (11), for a range of diverse molecular direction within the junction (know by the © 2024 The Author(s). Published by the Royal Society of Chemistry angle Q, :C ipso -S-Au) as shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Theoretical investigation of thermoelectric properties of methyl blue-based molecular junctions

Al-Mohana,

Najeeb,

Al-Utayjawee

et al. 2024

RSC Adv.

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“…Because many spectroscopic assays are affected by the presence of NMs, the use of dye-free methods avoids the issues presented. For example, flow cytometry, manual cell counting techniques, electrical cell-based impedance protocols [109] or clonogenic assays [110] can be used to assess viability. A significant issue is our current inability to predict the dominant mode of interference by each NM formulation on a particular assay, therefore, each assay will be differentially affected by the type and characteristics of the NM studied.…”

Section: Recommendationsmentioning

confidence: 99%

Aquatic toxicity of manufactured nanomaterials: challenges and recommendations for future toxicity testing

et al. 2014

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Environmental context. The increased use of nanomaterials in industrial and consumer products requires robust strategies to identify risks when they are released into the environment. Aquatic toxicologists are beginning to possess a clearer understanding of the chemical and physical properties of nanomaterials in solution, and which of the properties potentially affect the health of aquatic organisms. This review highlights the main challenges encountered in aquatic nanotoxicity testing, provides recommendations for overcoming these challenges, and discusses recent studies that have advanced our understanding of the toxicity of three important OECD nanomaterials, titanium dioxide, zinc oxide and silver nanomaterials.Abstract. Aquatic nanotoxicologists and ecotoxicologists have begun to identify the unique properties of the nanomaterials (NMs) that potentially affect the health of wildlife. In this review the scientific aims are to discuss the main challenges nanotoxicologists currently face in aquatic toxicity testing, including the transformations of NMs in aquatic test media (dissolution, aggregation and small molecule interactions), and modes of NM interference (optical interference, adsorption to assay components and generation of reactive oxygen species) on common toxicity assays. Three of the major OECD (Organisation for Economic Co-operation and Development) priority materials, titanium dioxide (TiO 2 ), zinc oxide (ZnO) and silver (Ag) NMs, studied recently by the Natural Sciences and Engineering Research Council of Canada (NSERC), National Research Council of Canada (NRC) and the Business Development Bank of Canada (BDC) Nanotechnology Initiative (NNBNI), a Canadian consortium, have been identified to cause both bulk effect, dissolution-based (i.e. free metal), or NM-specific toxicity in aquatic organisms. TiO 2 NMs are most toxic to algae, with toxicity being NM size-dependent and principally associated with binding of the materials to the organism. Conversely, dissolution of Zn and Ag NMs and the subsequent release of their ionic metal counterparts appear to represent the primary mode of toxicity to aquatic organisms for these NMs. In recent years, our understanding of the toxicological properties of these specific OECD relevant materials has increased significantly. Specifically, researchers have begun to alter their experimental design to identify the different behaviour of these materials as colloids and, by introducing appropriate controls and NM characterisation, aquatic nanotoxicologists are now beginning to possess a clearer understanding of the chemical and physical properties of these materials in solution, and how these materials may interact with organisms. Arming nanotoxicologists with this understanding, combined with knowledge of the physics, chemistry and biology of these materials is essential for maintaining the accuracy of all future toxicological assessments.

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Noninvasive Cell-Based Impedance Spectroscopy for Real-Time Probing Inhibitory Effects of Graphene Derivatives

Abstract: /npsi/ctrl?lang=en http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/ctrl?lang=fr Access and use of this website and the material on it are subject to the Terms and Conditions set forth at

Cited by 10 publications

References 44 publications

The Effects of Extensive Glomerular Filtration of Thin Graphene Oxide Sheets on Kidney Physiology

The Effects of Extensive Glomerular Filtration of Thin Graphene Oxide Sheets on Kidney Physiology

Theoretical investigation of thermoelectric properties of methyl blue-based molecular junctions

Aquatic toxicity of manufactured nanomaterials: challenges and recommendations for future toxicity testing

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