Nonspecific acid esterase activity: A criterion for differentation of T and B lymphocytes in mouse lymph nodes

Abstract

A modified technique was used to demonstrate lymphocytic acid alpha-naphthyl acetate esterase activity in frozen sections of mouse lymphoid tissue or smears. Positive, dot-like reaction products were noticed in more than 94% of the lymphocytes located in the diffuse cortical ("paracortical", "thymus-dependent") area of mesenteric lymph nodes of young adult ICR mice. Almost identical values were found for lymphocytes in the cisterna chyli. In contrast, the follicular cortex which is predominantly occupied by B cells, contained less than 7% esterase-positive lymphocytes. In vitro, cytotoxic anti-theta serum destroyed the vast majority of esterase positive lymphocytes while esterase-negative lymphocytes were resistent to this treatment. In mesenteric nodes of nude BALB/c (nu/nu) mice, follicular cortex and paracortex together contained approximately 16 times less esterase-positive lymphocytes than in the immunologically competent hybrid BALB/c (nu/+) animals. These findings indicate that non-specific acid esterase activity may serve as a criterion to differentiate peripheral T and B lymphocytes in lymph node sections and smears of mice by light microscopy. Possible implications of this enzyme activity in thymus-derived lymphocytes are discussed.

Immune functions of human synovial cells. phenotypic and t cell regulatory properties of macrophage‐like cells that express hla–dr

“…Frozen sections from normal and rheumatoid synovial tissue and cytocentrifuge preparations of cells obtained from normal and rheumatoid synovial specimens were investigated for the presence of acid alpha-naphtyl acetate esterase (ANAE) (22), naphthol AS-D acetate esterase (NASDAE), inhibitable by sodium fluoride (NaF) (23,24), and naphthol AS-D chloroacetate esterase (NASDCAE) (25). ATPase staining was performed on normal suspended synoviocytes (26).…”

Biochemical activities of nine lysosomal enzymes in T and non‐T lymphocytes

“…The first is a different differentiation of lysosomes, as an intracellular organelle, in T and non-T lymphocytes, reflecting their cellular functions. Mueller et al [7] reported that the cytochemical activity might be acquired by T lymphocytes just before, during/or shortly after peripheralization. Horwiz et al ]8] noted that a single intense reaction by acid esterase staining, which is characteristic in T lymphocytes, disappears after mitogen-induced transformation.…”

Enzyme histochemical, histometric and hematological features of peripheral blood cells in SparrowhawkAccipiter nisus(Falconiformes: Accipitridae)

“…The alpha naphtyl acetate esterase (ANAE) staining method is one of these tests, along with the enzymehistochemical analysis method, which provides different reactions to each of the blood cells (Mueller et al 1975;Higgy et al 1977;Basso et al 1980;Çelik et al 1994;Aştı et al 1996). It was determined that this lysosomal enzyme, which is a nonspecific esterase, is responsible for phagocytic and cytotoxic activities (Mueller et al 1975;Catowsky 1991). The image that is evaluated as positive in the leukocytes stained with the ANAE method is a homogeneous reaction of brown-red granules under the cell membrane or in cytoplasm.…”

“…The image that is evaluated as positive in the leukocytes stained with the ANAE method is a homogeneous reaction of brown-red granules under the cell membrane or in cytoplasm. This enzyme is generally stained with 1-5 granules in lymphocytes (Ozcan 2005;Karaca et al 2006;Şimşek et al 2009), with dense homogeneous granules in monocytes (Mueller et al 1975;Higgy et al 1977;Basso et al 1980;Ramos et al 1992), and with granulocytes, it is stained in different granular styles, which vary according to the positivity or negativity of the animal species. In this method, neutrophil granulocytes show a negative reaction in humans (Higgy et al 1977), dogs, cats and horses (Aştı et al 1996), and they show a positive reaction in rats, cattle (Aştı et al 1996), sheep and goats (Osbaldiston & Sullivan 1978).…”