Electrical impedance tomography (EIT) is a non-invasive, radiation-free imaging technique with a lot of promise in clinical monitoring. However, since EIT image reconstruction is a non-linear, pathological, and ill-posed issue, the quality of the reconstructed images needs constant improvement. To increase image reconstruction accuracy, a grey wolf optimized radial basis function neural network (GWO-RBFNN) is proposed in this paper. The grey wolf algorithm is used to optimize the weights in the radial base neural network, determine the mapping between the weights and the initial position of the grey wolf, and calculate the optimal position of the grey wolf to find the optimal solution for the weights, thus improving the image resolution of EIT imaging. COMSOL and MATLAB were used to numerically simulate the EIT system with 16 electrodes, producing 1700 simulation samples. The standard Landweber, RBFNN, and GWO-RBFNN approaches were used to train the sets separately. The obtained image correlation coefficient (ICC) of the test set after training with GWO-RBFNN is 0.9551. After adding 30, 40, and 50 dB of Gaussian white noise to the test set, the attained ICCs with GWO-RBFNN are 0.8966, 0.9197, and 0.9319, respectively. The findings reveal that the proposed GWO-RBFNN approach outperforms the existing methods when it comes to image reconstruction.