Nonstructural 5A gene variability of hepatitis C virus (HCV) during a 10-year follow up

Fan, Wenmei; Zhu, Weifeng; Wei, Lai; Wang, Qi-xin; Li-min, Yin; Du, Shiwei; Zhang, Hui

doi:10.1007/s00535-004-1446-2

Cited by 14 publications

(12 citation statements)

References 33 publications

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“…Nonsense mutations in samples from HCV-infected patients have been described [34,36,49], but the structural and functional implications of such mutations detected in vivo are unclear. Most of the nonsense mutations detected were located in the N-terminal region of NS5A (Alpha Helix, Domain I or Low Complexity Sequence I regions of the NS5A); only one was located in the C-terminal region (domain III).…”

Section: Discussionmentioning

confidence: 99%

Analysis of HCV quasispecies dynamic under selective pressure of combined therapy

et al. 2013

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BackgroundThe quasispecies composition of Hepatitis C virus (HCV) could have important implications with regard to viral persistence and response to interferon-based therapy. The complete NS5A was analyzed to evaluate whether the composition of NS5A quasispecies of HCV 1a/1b is related to responsiveness to combined interferon pegylated (PEG-IFN) and ribavirin therapy.MethodsViral RNA was isolated from serum samples collected before, during and after treatment from virological sustained responder (SVR), non-responder (NR) and the end-of-treatment responder patients (ETR). NS5A region was amplified, cloned and sequenced. Six hundred and ninety full-length NS5A sequences were analyzed.ResultsThis study provides evidence that lower nucleotide diversity of the NS5A region pre-therapy is associated with viral clearance. Analysis of samples of NRs and the ETRs time points showed that genetic diversity of populations tend to decrease over time. Post-therapy population of ETRs presented higher genetic distance from baseline probably due to the bottleneck phenomenon observed for those patients in the end of treatment. The viral effective population of those patients also showed a strong decrease after therapy. Otherwise, NRs demonstrated a continuous variation or stability of effective populations and genetic diversity over time that did not seem to be related to therapy. Phylogenetic relationships concerning complete NS5A sequences obtained from patients did not demonstrate clustering associated with specific response patterns. However, distinctive clustering of pre/post-therapy sequences was observed. In addition, the evolution of quasispecies over time was subjected to purifying or relaxed purifying selection. Codons 157 (P03), 182 and 440 (P42), 62 and 404 (P44) were found to be under positive selective pressure but it failed to be related to the therapy.ConclusionThese results confirm the hypothesis that a relationship exists between NS5A heterogeneity and response to therapy in patients infected with chronic hepatitis C.

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Section: Discussionmentioning

confidence: 99%

Analysis of HCV quasispecies dynamic under selective pressure of combined therapy

et al. 2013

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“…Fan et al (34) found that the functional domain of NS5A remained conserved over an interval of 10 years of follow-up. Similarly, other studies showed that, in patients without HCC, the NS5A-PKR sequences did not change considerably during the natural course of liver disease (35), and the variable quasispecies structure of NS5A remains largely stable, independent of the degree of liver disease (36).…”

Section: Discussionmentioning

confidence: 99%

Evolution of hepatitis C virus non‐structural 5A gene in the progression of liver disease to hepatocellular carcinoma

Mitri

Cassini

Bagaglio

et al. 2007

Liver International

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“…5 0 UTR is a more conserved part of HCV genome, which helped in evolutionary studies and genotyping [11,12].…”

Section: History and Structure Of Hepatitis C Virusmentioning

confidence: 99%

An overview on hepatitis C virus genotypes and its control

Nouroz

Shaheen

Mujtaba

et al. 2015

Egyptian Journal of Medical Human Genetics

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Hepatitis C virus (HCV) is a blood borne, circular and positive single stranded virus with high spread rates. With the passage of time the frequency of HCV is increasing in different parts of the world. HCV is a major cause, which may end in liver cirrhosis and hepatocellular carcinoma. HCV has six main genotypes with many subtypes, which have variable sequence homology with each other. Symptoms can appear anytime from 2 weeks to 6 months, which include jaundice, fatigue, gray-colored stool, joint pain, belly pain, weakness, anorexia, itchy skin and dark urine. Genotyping is more significant for planning of HCV treatment period and helps to cure HCV infections. For the quantification and identification of hepatitis C virus-ribonucleic acid, many molecular techniques are performed; the most significant are HCV ELISA, quantitative HCV-RNA PCR and recombinant immunoblot assay. PCR is the major technique targeting 5 0 untranslated region (UTR). HCV can be transmitted by contaminated blood, ear and nose piercing and contaminated medical instruments. To overcome the rate of HCV, guidance should be provided to make aware the persons about risk factors, transmission and prevention. Discovery and designing of new therapies and vaccines to overcome this disease are the necessity of the present era. Four types of vaccines such as vector vaccines, peptide vaccines, DNA vaccines and recombinant protein vaccines are available in clinical trials.

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Nonstructural 5A gene variability of hepatitis C virus (HCV) during a 10-year follow up

Cited by 14 publications

References 33 publications

Analysis of HCV quasispecies dynamic under selective pressure of combined therapy

Analysis of HCV quasispecies dynamic under selective pressure of combined therapy

Evolution of hepatitis C virus non‐structural 5A gene in the progression of liver disease to hepatocellular carcinoma

An overview on hepatitis C virus genotypes and its control

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