Normal and Tumour Tissue mRNA Expressions of Telomerase Complex Genes in Several Types of Cancer

Abstract

Aims:To investigate the changes in mRNA expression levels of telomerase-related significant proteins in several types of cancer.Methods:Human telomerase reverse transcriptase, pontin, reptin and dyskerin expressions were measured in normal and tumour tissues obtained from 26 patients with colorectal, breast and gastric cancers, using the real-time reverse transcriptase-polymerase chain reaction method.Results:For all patients, no significant difference was found in mRNA expressions of human telomerase reverse transcriptase and dyskerin (p>0.05), although their levels in tumour tissues were found to be higher than in normal tissues. However, pontin and reptin mRNA expressions were significantly higher in tumour tissues than in normal tissues (p<0.01). While human telomerase reverse transcriptase showed a high correlation with only pontin (p<0.001) in normal tissues, high positive correlations were observed between human telomerase reverse transcriptase with pontin (p<0.005), reptin (p<0.01) and dyskerin (p<0.01) in tumour tissues.Conclusion:The increased mRNA expressions of all four genes in tumour tissues may suggest a role in cancer development. Correlations of pontin, reptin and dyskerin with human telomerase reverse transcriptase support the hypotheses describing their roles in telomerase complexes.

The mechanism of traditional Chinese medicine in the treatment of gastric cancer using the method of tonifying qi, removing blood stasis, and detoxifying

“…Caliskan et al [5] found that telomerase activity regulates the expression of human telomerase reverse transcriptase (hTERT) in GC cells, and proposed that inhibiting telomerase activity may be a potential Another study evaluated the telomerase activity of BGC-823 human GC cells using the PCR-TRAP method and found that gambogic acid (GA) can inhibit telomerase activity by inhibiting Akt phosphorylation and reducing hTERT expression. Duan et al [6] confirmed that piperonamide (PL) exerts anti-cancer effects by inhibiting telomerase activity and cell proliferation.…”

<p>Mechanisms Underlying Therapeutic Effects Of Traditional Chinese Medicine On Gastric Cancer</p>

“…Çalışkan et al17 found that telomerase activity regulates the expression of human telomerase reverse transcriptase (hTERT) in gastric cancer cells, and suggested that inhibiting telomerase activity may be a potential anti-cancer therapeutic mechanism. Another study18 utilized the PCR-TRAP method to evaluate telomerase activity in BGC-823 human gastric carcinoma cells, and found that gambogic acid (GA) can inhibit telomerase activity by inhibiting the phosphorylation of Akt and reducing the expression of hTERT.…”