Normal phase liquid chromatography–electrospray ionization tandem mass spectrometry analysis of phospholipid molecular species in blood mononuclear cells: application to cystic fibrosis

Application of the accurate mass and time tag approach in studies of the human blood lipidome

“…The majority of these species have been previously reported in studies of these tissue types [46][47][48][49][50][51]. After identification of lipid species were made, further MS/MS analyses were unnecessary, and highthroughput LC-TOF MS analyses were utilized to maximize analysis throughput and quantitative sensitivity.…”

Characterization of Phospholipid Molecular Species by Means of HPLC-Tandem Mass Spectrometry

“…Kakela et al (2003) used hexane/isopropanol/formic acid/triethylamine. Malavolta et al (2004) used chloroform/methanol/water/ammmonium hydroxide. Commonly, NP HPLC requires an eluent gradient or one or more changes of the eluent mixture (Becart et al 1990;Fagan & Wijesundera, 2004;Hemström & Irgum, 2006;Bang et al, 2007).…”

“…Since this fragment is characteristic of the hexadecyl vinyl ether group linked in sn-1 position, it is possible to detect all the pPE molecular species containing this substituent by using a precursor ion scan at m/z= 364 or all pPE species with an octadecyl vinyl ether by a precursor ion scan of m/z = 392. The characterization of pPE molecular species by means of ESI working in negative ionmode is reported by Malavolta et al (2004) and by Hsu & Turk 2009. Both groups observed the [M-H] -ions in negative mode but the product ion spectra obtained by the two authors are different.…”

“…Molecular ions produced by ESI in negative mode corresponded to [M-H] -. Collisional activation of PI negative molecular ion species [M-H] -was reviewed by several authors (Hsu & Turk, 2009;Malavolta et al, 2004). They observed that the MS 2 -spectrum of [M-H] The latest resulted the most abundant ion.…”

A review of chromatographic methods for the assessment of phospholipids in biological samples

“…The process of derivatizing may modify the structure of the polyunsaturated fatty acids (PUFAs; Eder, 1995) or cause artifacts that overlap with the FAME peaks (Malavolta et al, 2004b). Also, the short chain FAMES are very volatile and may be lost in this step (Lima, 2002).…”