NOS Expression in Oxidative Stress, Neurodegeneration and Male Infertility Induced by the Abuse of Tramadol

Aea, El Baky; Hafez, MM

doi:10.4172/2167-0501.1000223

Cited by 5 publications

(6 citation statements)

References 27 publications

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“…Oxidative stress with overexpression of nitric oxide synthase (NOS) are declared as the most important possible pathophysiology for tramadol-induced suppressive impact on spermatogenesis added to spermiological parameters (El-Baky A. and Hafez M., 2017). Oxidative stress conditions have also been shown to initiate progressive aponecrosis, necrosis, and ferroptosis in male testicular tissue, resulting in male fertility loss (Alahmar 2019).…”

Section: Discussionmentioning

confidence: 99%

The possible protective effect of the nuclear factor kappa B inhibitor pyrrolidine dithiocarbamate on tramadol-induced testicular damage in rats

Mahmoud

Moursi

Esmaeel

et al. 2023

Egypt J Forensic Sci

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Background Tramadol provokes oxidative stress that gives rise to apoptosis with alterations in the cellular structure and adversely influences male fertility. A specific inhibitor of nuclear factor kappa B (NF-κB), pyrrolidine dithiocarbamate (PDTC), has observable antioxidant and anti-inflammatory characteristics and enhances the improvement of organs damage caused by various agents. The impact of PDTC on testicular damage caused by tramadol has not been previously examined. Objective This study was designed to investigate the potential impact of pyrrolidine dithiocarbamate on testicular damage provoked by chronic tramadol usage. Materials and methods Forty healthy adult male albino rats were included in this study. Rats were randomly and equally divided into 4 groups: group (I), control group; group (II), pyrrolidine dithiocarbamate (PDTC) group; group (III), tramadol (Tr) group; and group (IV), Tr + PDTC group. This study measured serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), and free testosterone levels. Testicular malondialdehyde (MDA), interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) levels, and superoxide dismutase (SOD), glutathione peroxidase (GPX), and caspase-8 and caspase-3 activities were also detected. Immuno-histochemical evaluation of inducible NOS (iNOS) expression in testicular tissue along with histopathological examination of testicular tissue has been studied. Results Tramadol caused a significant reduction in serum FSH, LH and testosterone levels, epididymal sperm count, and motility, as well as testicular GPX and SOD activities. On the other hand, a significant elevation of testicular MDA, IL-1β, IL-6, and TNF-α levels and caspase-8 and caspase-3 activities were found. However, PDTC administration with tramadol showed significantly increased sperm production and motility and alleviated tramadol-induced disturbance in other measured parameters in the Tr + PDTC group compared to the Tr group. Moreover, co-administration of PDTC with tramadol significantly alleviated the histopathological structure of testicular tissue and the increased iNOS expressions noticed in the tramadol-treated group. Conclusion Considering the protective effects of PDTC against the reproductive toxicity induced by tramadol, this compound can be used as a possible protective and treating target for tramadol-induced reproductive toxicity.

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Section: Discussionmentioning

confidence: 99%

The possible protective effect of the nuclear factor kappa B inhibitor pyrrolidine dithiocarbamate on tramadol-induced testicular damage in rats

Mahmoud

Moursi

Esmaeel

et al. 2023

Egypt J Forensic Sci

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“…TAM was impressively capable of implementing its malignant activities in the production of the testis as well as the function and, thus, the regulation of serum hormonal levels that regulate the male richness [27,36,37]. Furthermore, Abdel-Hamid et al [7] suggested that TAM may exhibit a beneficial effect on premature ejaculation therapy. Rats treated with E. brevicornum at a dose of 2.43 g/kg/day for 130 consecutive days elicited a remarkable increase in sperm motility and count, with a marked decrease in sperm abnormalities compared to the control or TAM-treated groups.…”

Section: Discussionmentioning

confidence: 99%

“…TAM has been reported to cause respiratory depression and psychological and physiological addiction [5]. Moreover, long-term administration of TAM results in an increase in oxidative stress that causes male infertility due to low levels of testosterone and neurodegenerative diseases such as Alzheimer's disease [6,7]. There is a disturbing increase in the abuse of TAM, mainly among young people assuming that it improves sexual excitement and climax without the development of sexual dysfunctions [8], and small quantities for additional investigations and examinations have been implanted [9].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, long-term administration of TAM results in an increase in oxidative stress that causes male infertility due to low levels of testosterone and neurodegenerative diseases such as Alzheimer's disease [5,6]. There is a disturbing increase in the abuse of TAM, mainly among young people assuming that it improves sexual excitement and climax without the development of sexual dysfunctions [7], and small quantities for additional investigations and examinations have been implanted [8]. There is an urgent need for safer natural antioxidants to minimize the effects of oxidative and peroxidative damage of TAM on male infertility.…”

Section: Introductionmentioning

confidence: 99%

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Chemotherapeutic Potential of Epimedium brevicornum Extract: The cGMP-Specific PDE5 Inhibitor as Anti-Infertility Agent Following Long-Term Administration of Tramadol in Male Rats

et al. 2020

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Epimedium brevicornum Maxim (EbM) is a well-known Chinese herb that has been widely used for the treatment of several diseases. The main purpose of this study is to examine the role of Epimedium brevicornum extract in certain andrological parameters in rats as a natural modulator for adverse viewpoints associated with chronic administration of tramadol (TAM). Fifty rats were categorized into five groups. Untreated rats were known as Group I, whereas rats in Groups II and III were administered 2.43 g/kg/day of E. brevicornum extract and 50 mg/kg/day of TAM for 130 consecutive days, respectively. Both of Groups IV and V were administered TAM for 65 successive days, followed by concomitant use of both drugs for another 65 days, with the E. brevicornum extract at doses of 0.81 and 2.43 g/kg/day, respectively. TAM showed an injurious effect on sperm attributes, serum hormones, tissue malondialdehyde, superoxide dismutase, and nitric oxide. Elevation of the apoptotic marker Bax and a reduction of Bcl2 were recorded. Histopathological abnormalities have been reported in rat testicles. Rats treated with E. brevicornum extract with TAM showed an improvement in all the parameters tested. It could be presumed that E. brevicornum extract plus TAM exhibits a promising effect on the enhancement of male anti-infertility effects.

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“…This is marked in the body of boars by a decrease in the activity of SOD and CAT (Shostya et al, 2020). The decrease in CAT activity occurs due to the high sensitivity to NO, the content of which increases significantly under the influence of OS (El-Baky & Hafez, 2017). It is known that SOD provides catalase with a substrate -hydrogen peroxide, however the activity of both enzymes was significantly reduced.…”

Section: Discussionmentioning

confidence: 99%

The activity of the antioxidant protection enzymatic system of boars with a decrease in their reproductive capacity under oxidative stress

Koshevoy¹,

Naumenko²

2020

Theor. Appl. Vet. Med.

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It was found that enzymatic system activity of the antioxidant protection of the breeding boars organism with a decrease in their reproductive ability under oxidative stress was significantly decreased, which was noted by a decrease in the antioxidant capacity. Males of the experimental group had a decrease in superoxide dismutase activity by 30,2% (p ˂ 0,01). At the same time, in the erythrocytes of breeding boars, catalase activity was reduced by 33,9% (p ˂ 0,001) compared with the indicators of the control group animals, due to the high sensitivity to nitric oxide, the content of which was significantly increased during oxidative stress; interacting with nitric oxide, a suppressed form of catalase – ferricatalase-NO was formed. A decrease in the activity of these enzymes is characteristic of the oxidative stress development – which is due to the high level of peroxidation processes in the body of breeding boars. With a decrease in the activity of glutathione system components, an imbalance of the prooxidant-antioxidant system was noted. According to the research results, a decrease in the activity of the glutathione redox cycle enzymes – glutathione peroxidase and glutathione reductase was noted, which in boars with a decrease of reproductive ability were also reduced, and this also confirmed the state of oxidative stress. Thus, boars of the experimental group had a pronounced decrease in glutathione peroxidase activity by 28,4% (p ˂ 0,05), while the activity of glutathione reductase in erythrocytes was significantly reduced by 20,2% (p ˂ 0,05). The lack of physiological activity of antioxidant enzymes will have a negative impact on the fertilizing ability of sperm, because the lack of components of the enzymatic antioxidant defence system affects the activity of enzymes in sperm received from the breeding boars. A balance was observed between the components of the enzymatic antioxidant defense: the ratio of antioxidant enzymes’ activity in the male experimental group was lower than the values in the control group, however, the indices of superoxide dismutase/catalase and glutathione peroxidase/glutathione reductase had the same values, indicating that the balance in the enzymatic system of antioxidant defense was maintained. A decrease in enzyme indices in the experimental group indicated a decrease in the antioxidant potential and confirmed a certain state of oxidative stress in the breeding boars organism.

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NOS Expression in Oxidative Stress, Neurodegeneration and Male Infertility Induced by the Abuse of Tramadol

Cited by 5 publications

References 27 publications

The possible protective effect of the nuclear factor kappa B inhibitor pyrrolidine dithiocarbamate on tramadol-induced testicular damage in rats

The possible protective effect of the nuclear factor kappa B inhibitor pyrrolidine dithiocarbamate on tramadol-induced testicular damage in rats

Chemotherapeutic Potential of Epimedium brevicornum Extract: The cGMP-Specific PDE5 Inhibitor as Anti-Infertility Agent Following Long-Term Administration of Tramadol in Male Rats

The activity of the antioxidant protection enzymatic system of boars with a decrease in their reproductive capacity under oxidative stress

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