Nosocomial Spread of Hepatitis B Virus in Two Hemodialysis Units, Investigated by Restriction Fragment Length Polymorphism Analysis

Hepatitis B virus infection profile in hemodialysis patients in Central Brazil: prevalence, risk factors, and genotypes

“…Genotype D was predominant in this hemodialysis patients (61.5%), as observed by others (De Castro et al 2000, Teles et al 2002). In addition, HBV isolates from genotype D have been involved in outbreak in dialysis units (De Castro et al 2000). These findings suggest that genotype D is more likely to disseminate in hemodialysis environmental.…”

“…These genotypes have been found in other Brazilian hemodialysis populations (De Castro et al 2000, Teles et al 2002, Souza et al 2003 reflecting the HBV genotypes circulating in our country (Moraes et al 1996, Araujo et al 2004, Sitnik et al 2004. Genotype D was predominant in this hemodialysis patients (61.5%), as observed by others (De Castro et al 2000, Teles et al 2002). In addition, HBV isolates from genotype D have been involved in outbreak in dialysis units (De Castro et al 2000).…”

“…However, for others, HBV infection is still an important problem to patients (Vladutiu et al 2000, Otedo et al 2003, Kumar et al 2005. Nevertheless, HBV infection outbreaks are still seen when failures in infection control procedures occur (De Castro et al 2000, Lewis-Ximenez et al 2001.…”

[No Title Available]

“…HBV genotypes A, D and F have been found in Brazilian hemodialysis population (De Castro et al 2000, Carrilho 2001, Teles et al 2002, reflecting the HBV genotypes circulating in our country (Moraes et al 1996). In the present study, only HBV genotype A strains were detected in the hemodialysis patients.…”

“…Four primers pairs were used to amplify HBV fragments which, taken together, encompassed 81% of the genome: PS1-PS2 and X1-X2 (Niel et al 1994), C1-PS2 and PS4-S2 . The PCR products were subjected to RFLP analysis, as previously described by De Castro et al (2000).…”