Not just a marker: CD34 on human hematopoietic stem/progenitor cells dominates vascular selectin binding along with CD44

AbuSamra, Dina B.; Aleisa, Fajr A.; Al-Amoodi, Asma S.; Ahmed, Heba M. Jalal; Chin, Chee Jia; Abuelela, Ayman F.; Bergam, Ptissam; Sougrat, Rachid; Merzaban, Jasmeen S.

doi:10.1182/bloodadvances.2017004317

Cited by 85 publications

(103 citation statements)

References 90 publications

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“…Proliferation and differentiation of hematopoietic stem/progenitor cells are known to depend on specific local microenvironment formed by bone marrow vascular niches [ 5 , 6 ]. Endothelium of bone marrow is found to express cell adhesion molecules involved in cell signaling by interaction with glycoprotein and glycolipid ligands of HSPCs [ 6 , 7 , 8 ]. Recently, it was shown that selectins and integrins play a crucial role in regulation of hematopoiesis in experimental animals with myelosuppression after chemotherapy [ 9 , 10 , 20 , 21 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of Modified Fucoidan and Related Sulfated Oligosaccharides on Hematopoiesis in Cyclophosphamide-Induced Mice

Anisimova¹,

Ustyuzhanina

Bilan

et al. 2018

Marine Drugs

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Immunosuppression derived after cytostatics application in cancer chemotherapy is considered as an adverse side effect that leads to deterioration of quality of life and risk of infectious diseases. A linear sulfated (1→3)-α-l-fucan M-Fuc prepared by chemical modification of a fucoidan isolated from the brown seaweed Chordaria flagelliformis, along with two structurally related synthetic sulfated oligosaccharides, were studied as stimulators of hematopoiesis on a model of cyclophosphamide immunosuppression in mice. Recombinant granulocyte colony-stimulating factor (r G-CSF), which is currently applied in medicine to treat low blood neutrophils, was used as a reference. Polysaccharide M-Fuc and sulfated difucoside DS did not demonstrate significant effect, while sulfated octasaccharide OS showed higher activity than r G-CSF, causing pronounced neutropoiesis stimulation. In addition, production of erythrocytes and platelets was enhanced after the octasaccharide administration. The assessment of populations of cells in blood and bone marrow of mice revealed the difference in mechanisms of action of OS and r G-CSF.

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Section: Discussionmentioning

confidence: 99%

Influence of Modified Fucoidan and Related Sulfated Oligosaccharides on Hematopoiesis in Cyclophosphamide-Induced Mice

Anisimova¹,

Ustyuzhanina

Bilan

et al. 2018

Marine Drugs

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show abstract

“…The cell-by-cell analysis and mixture modeling suggests that this ensemble average does not capture the subpopulations having high-PDGFR plasma membrane localization: 66 and 16% of mouse tumor EC-like cell membrane had ~23,400 PDGFRα and ~19,800 PDGFRβ, respectively (Figure 2F ). This significant heterogeneity may be attributed to the use of the CD34 marker to designate EC-like cells, because it is also found on stem cells/precursors, mast cells, and neurons (Nielsen and McNagny, 2008 ; Imoukhuede and Popel, 2014 ; AbuSamra et al, 2017 ). PDGFRα is also considered an important mesenchymal stem cell marker (Farahani and Xaymardan, 2015 ).…”

Section: A New Approach For Examining Gbm Heterogeneitymentioning

confidence: 99%

Characterizing Glioblastoma Heterogeneity via Single-Cell Receptor Quantification

Chen

Harley

et al. 2018

Front. Bioeng. Biotechnol.

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Dysregulation of tyrosine kinase receptor (RTK) signaling pathways play important roles in glioblastoma (GBM). However, therapies targeting these signaling pathways have not been successful, partially because of drug resistance. Increasing evidence suggests that tumor heterogeneity, more specifically, GBM-associated stem and endothelial cell heterogeneity, may contribute to drug resistance. In this perspective article, we introduce a high-throughput, quantitative approach to profile plasma membrane RTKs on single cells. First, we review the roles of RTKs in cancer. Then, we discuss the sources of cell heterogeneity in GBM, providing context to the key cells directing resistance to drugs. Finally, we present our provisionally patented qFlow cytometry approach, and report results of a “proof of concept” patient-derived xenograft GBM study.

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“…However, robust data highlight a CD34 expression not only by MSCs but by several other non-hematopoietic cell types [37]. The CD34 positive cells may define a specific subset of progenitor cells with enhanced adhesive and homing properties, which are likely to mimic lymphocyte homing to the inflammatory sites [38,39].…”

Section: Discussionmentioning

confidence: 99%

The Impact of Cell-Expansion and Inflammation on The Immune-Biology of Human Adipose Tissue-Derived Mesenchymal Stromal Cells

Buyl

Merimi

Rodrigues

et al. 2020

JCM

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Background: As a cell-based therapeutic, AT-MSCs need to create an immuno-reparative environment appropriate for tissue repair. In the presence of injury, MSCs may have to proliferate and face inflammation. Clinical application requires repeated administrations of a high number of cells with a well-established immune profile. Methods: We have established an immuno-comparative screening by determining the expression of 28 molecules implicated in immune regulation. This screening was performed during cell-expansion and inflammatory priming of AT-MSCs. Results: Our study confirms that AT-MSCs are highly expandable and sensitive to inflammation. Both conditions have substantially modulated the expression of a panel of immunological marker. Specifically, CD34 expression was substantially decreased upon cell-passaging. HLA-ABC, CD40 CD54, CD106, CD274 and CD112 were significantly increased by inflammation. In vitro cell-expansion also significantly altered the expression profile of HLA-DR, CD40, CD62L, CD106, CD166, HLA-G, CD200, HO-1, CD155 and ULBP-3. Conclusion: This study points out the response and characteristics of MSCs following expansion and inflammatory priming. It will strength our knowledge about the molecular mechanisms that may improve or hamper the therapeutic potential of MSCs. These immunological changes need to be further characterized to guarantee a safe cellular product with consistent quality and high therapeutic efficacy.

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Not just a marker: CD34 on human hematopoietic stem/progenitor cells dominates vascular selectin binding along with CD44

Abstract: Key Points• Human HSPCs expressing CD34 exhibit E-selectin binding activity, whereas those lacking CD34 do not.• CD34 is a unique E-and P-selectin ligand on human HSPCs that binds with kinetics comparable to other known selectin ligands.

Cited by 85 publications

References 90 publications

Influence of Modified Fucoidan and Related Sulfated Oligosaccharides on Hematopoiesis in Cyclophosphamide-Induced Mice

Influence of Modified Fucoidan and Related Sulfated Oligosaccharides on Hematopoiesis in Cyclophosphamide-Induced Mice

Characterizing Glioblastoma Heterogeneity via Single-Cell Receptor Quantification

The Impact of Cell-Expansion and Inflammation on The Immune-Biology of Human Adipose Tissue-Derived Mesenchymal Stromal Cells

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