Not so fast: Paradoxically increased variability in the glucose tolerance test due to food withdrawal in continuous glucose-monitored mice

Rubio, William B.; Cortopassi, Marissa D.; Ramachandran, Deepti; Walker, Samuel J.; Balough, Elizabeth M.; Wang, Jiefu; Banks, Alexander S.

doi:10.1016/j.molmet.2023.101795

Cited by 11 publications

(2 citation statements)

References 38 publications

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“…An injection of pyruvate can result in torpor, which is a state of diminished body temperature and activity, in obese mice ( Soto et al 2018 ). Torpor can also affect glucose metabolism ( Rubio et al 2023 ); hence, torpor can be a confounding variable during PTTs. More sophisticated yet complex techniques for quantification of gluconeogenesis in vivo exist ( Chevalier et al 2006 , Yook et al 2023 ).…”

Section: Determining Insulin Sensitivity and Glucose Metabolism ...mentioning

confidence: 99%

“…Continuous glucose monitoring (CGM) involves invasive surgery, namely, surgical implantation of a telemetry probe in the aorta ( Evers et al 2020 , Kennard et al 2021 , Rubio et al 2023 ). Assuming surgical expertise and funds exist in a laboratory, CGM is a great way to determine circulating glucose concentrations in rats and mice over weeks without handling them ( Evers et al 2020 , Kennard et al 2021 , Rubio et al 2023 ).…”

Section: Factors To Consider When Assessing Glucose Metabolism ...mentioning

confidence: 99%

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In vivo techniques for assessment of insulin sensitivity and glucose metabolism

Hahn,

Giacca,

Pereira

2024

Journal of Endocrinology

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Metabolic tests are vital to determine in vivo insulin sensitivity and glucose metabolism in preclinical models, usually rodents. Such tests include glucose tolerance tests, insulin tolerance tests, and glucose clamps. Although these tests are not standardized, there are general guidelines for their completion and analysis that are constantly being refined. In this review, we describe metabolic tests in rodents as well as factors to consider when designing and performing them.

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Section: Determining Insulin Sensitivity and Glucose Metabolism ...mentioning

confidence: 99%

Section: Factors To Consider When Assessing Glucose Metabolism ...mentioning

confidence: 99%

In vivo techniques for assessment of insulin sensitivity and glucose metabolism

Hahn,

Giacca,

Pereira

2024

Journal of Endocrinology

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Photobiomodulation Enhances the Effect of Strength Training on Insulin Resistance Regardless of Exercise Volume in Mice Fed a High‐Fat Diet

Costa,

Silva,

Guimarães

et al. 2024

Journal of Biophotonics

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The aim was to investigate the effects of different volumes of strength training (ST) in association with photobiomodulation (PBMt) in mice fed a high‐fat diet (HFD) on insulin resistance (IR). Male Swiss albino mice were fed HFD and performed high‐ or low‐volume (one‐third) ST (3 days/week), associated with PBMt (660 nm + 850 nm; ~42 J delivered) or not (lights off). ST improved IR, lowered visceral adiposity and circulating cytokines, and increased skeletal muscle hypertrophy and mitochondrial activity. The smaller volume of ST did not interfere with the improvement in IR, mitochondrial activity, or inflammatory profile, but exerted a smaller effect on visceral adiposity and skeletal muscle hypertrophy. Association with PBMt further improved IR, regardless of ST volume, although it did not affect adiposity, mitochondrial activity, and the inflammatory profile. Interestingly, PBMt positively affected quadriceps, but attenuated gluteus maximus hypertrophy. The association with PBMt induced greater improvement than ST alone.

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