Notch activates sonic hedgehog and both are involved in the specification of dorsal midline cell-fates in<i>Xenopus</i>

López, Silvia L.; Paganelli, A.; Siri, María Victoria Rosato; Ocaña, Oscar H.; Franco, Paula; Carrasco, Andrés

doi:10.1242/dev.00443

Cited by 52 publications

(69 citation statements)

References 89 publications

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“…There is strong evidence for a conserved role of Notch signaling in regulating hypochord allocation during gastrulation, but the signals regulating medial floor plate allocation are less clear (Appel et al, 1999;Latimer and Appel, 2006;Latimer et al, 2002;Peyrot et al, 2011). Speciesspecific roles for Notch and Midkine signaling have been identified, but a conserved pathway regulating this process across all species has yet to be identified (Gray and Dale, 2010;Lopez et al, 2003Lopez et al, , 2005Peyrot et al, 2011;Schafer et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

The zebrafish tailbud contains two independent populations of midline progenitor cells that maintain long-term germ layer plasticity and differentiate based on local signaling cues

et al. 2015

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Vertebrate body axis formation depends on a population of bipotential neuromesodermal cells along the posterior wall of the tailbud that make a germ layer decision after gastrulation to form spinal cord and mesoderm. Despite exhibiting germ layer plasticity, these cells never give rise to midline tissues of the notochord, floor plate and dorsal endoderm, raising the question of whether midline tissues also arise from basal posterior progenitors after gastrulation. We show in zebrafish that local posterior signals specify germ layer fate in two basal tailbud midline progenitor populations. Wnt signaling induces notochord within a population of notochord/floor plate bipotential cells through negative transcriptional regulation of sox2. Notch signaling, required for hypochord induction during gastrulation, continues to act in the tailbud to specify hypochord from a notochord/hypochord bipotential cell population. Our results lend strong support to a continuous allocation model of midline tissue formation in zebrafish, and provide an embryological basis for zebrafish and mouse bifurcated notochord phenotypes as well as the rare human congenital split notochord syndrome. We demonstrate developmental equivalency between the tailbud progenitor cell populations. Midline progenitors can be transfated from notochord to somite fate after gastrulation by ectopic expression of msgn1, a master regulator of paraxial mesoderm fate, or if transplanted into the bipotential progenitors that normally give rise to somites. Our results indicate that the entire non-epidermal posterior body is derived from discrete, basal tailbud cell populations. These cells remain receptive to extracellular cues after gastrulation and continue to make basic germ layer decisions.

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Section: Introductionmentioning

confidence: 99%

The zebrafish tailbud contains two independent populations of midline progenitor cells that maintain long-term germ layer plasticity and differentiate based on local signaling cues

et al. 2015

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“…In recent studies, the activation of Notch signaling by NICD decreases notochordal markers, such as Xbrachyury and chordin expression, in the dorsal midline during the Xenopus neurula stage (Lopez et al, 2003). Other studies indicate that Notch signaling is involved in the midline structure in zebrafish (Appel et al, 1999;Latimer et al, 2002).…”

Section: Regulation Of Xbrachyury By Xsu(h)2mentioning

confidence: 95%

“…Xotch is expressed ubiquitously at the early gastrula stage and is required for muscle formation (Coffman'et al, 1993). Notch signaling is involved in notochord patterning in the midline and L-R patterning in the lateral plate mesoderm (Latimer et al, 2002;Lopez et al, 2003Lopez et al, , 2005Raya et al, 2004); however, there is little information about the function of Notch signaling in germ layer formation during earliest embryogenesis. These results suggest that Notch signaling plays an active role in the cell fate decision in gastrula embryos.…”

Section: Introductionmentioning

confidence: 99%

XSu(H)2 is an essential factor for gene expression and morphogenesis of the Xenopus gastrula embryo

Ito

Katada²,

Miyatani³

et al. 2007

Int. J. Dev. Biol.

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“…Whether the choice of Hensen's node zone c cells to enter either the floor plate or the notochord differentiation pathway results from intrinsic cell fate decision and/or from extrinsic signals cannot be deduced from the previous data. However, recent results in several animal models are in favor of a balance between notochord and floor plate cells by cell fate decision at the level of the organizer (Appel et al, 1999;Lopez et al, 2003;Przemeck et al, 2003). Notch-Delta signaling is a good candidate for controlling this process.…”

Section: Hensen's Node Cell Fate and Differentiationmentioning

confidence: 99%

“…It has been shown that dlA mutants have a reduced number of floor plate cells and an excess of notochord cells, whereas embryos that over-express dlA have a reduced number of trunk notochord cells and an excess of floor plate cells. More recently, the role of Notch signaling was analyzed during specification of the dorsal midline in Xenopus embryos (Lopez et al, 2003). By activating or blocking Notch signaling, the authors found that Notch expands the floor plate domain of Xenopus embryos by stimulating Shh and pintallavis (HNF3β) and represses the notochordal markers chordin and brachyury.…”

Section: Hensen's Node Cell Fate and Differentiationmentioning

confidence: 99%

Cellular dynamics and molecular control of the development of organizer-derived cells in quail-chick chimeras

Charrier¹,

Catala²,

Lapointe³

et al. 2005

Int. J. Dev. Biol.

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Malformations affecting the nervous system in humans are numerous and various in etiology. Many are due to genetic deficiencies or mechanical accidents occurring at early stages of development. It is thus of interest to reproduce such human malformations in animal models. The avian embryo is particularly suitable for researching the role of morphogenetic movements and genetic signaling during early neurogenesis. The last ten years of research with Nicole Le Douarin in the Nogent Institut have brought answers to questions formulated by Etienne Wolff at the beginning of his career, by showing that Hensen's node, the avian organizer, is at the source of all the midline cells of the embryo and ensures cell survival, growth and differentiation of neural and mesodermal tissues. KEY WORDS: neurulation, Hensen's node, floor plate, quail-chick chimera, caudal dysplasia ForewordEtienne Wolff, the founder of the Nogent Institute, was a pioneer in teratogenic studies. He was the first to use the chick embryo as an animal model to construct phenocopies of human developmental malformations (Wolff, 1936). During his PhD thesis under the supervision of Paul Ancel in Strasbourg University, he experimentally reproduced cyclopia and symmelia, two dramatic malformations occasionally observed in human embryos, affecting organ bilaterality. He obtained phenocopies of such malformations in bird embryos by performing axial organ destruction using X irradiation at early stages of development (Fig. 1). Sixty years after these pioneer experiments, with Nicole Le Douarin, a former student of Etienne Wolff and his successor at the head of the Nogent Institute, we used the quail-chick cell labeling technique (Le Douarin, 1969) and microsurgical excision to elucidate the role of the avian organizer, Hensen's node, during the development of the central nervous system in the avian embryo. The results that we obtained confirmed and extended Wolff's observations and, like them, could be related to known human malformations. In the present paper we will review several aspects of our recent work, the aim of which was to improve our general understanding of the early development of the vertebrate nervous system. The first stages of neurogenesis: «primary» versus «secondary» neurulationIn birds, as in other chordates, a region of the primitive ectoderm is precociously induced to form the neurectoderm (Stern, 2002 for a review). Neural induction leads to the formation of a prismatic cell layer organized into the so-called neural plate. This plate will undergo a series of subsequent morphogenetic movements called neurulation, giving rise to the neural tube, i.e., the primordium of the central nervous system (CNS). Vertebrate neurulation proceeds according to two different mechanisms regarding the type of cell movements implicated (Catala et al., 1995;Colas and Schoenwolf, 2001 and references therein). In the first, which affects the anterior moiety of the bird embryo, the neural plate bends due to the formation of several hinge points (one me...

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Notch activates sonic hedgehog and both are involved in the specification of dorsal midline cell-fates inXenopus

Cited by 52 publications

References 89 publications

The zebrafish tailbud contains two independent populations of midline progenitor cells that maintain long-term germ layer plasticity and differentiate based on local signaling cues

The zebrafish tailbud contains two independent populations of midline progenitor cells that maintain long-term germ layer plasticity and differentiate based on local signaling cues

XSu(H)2 is an essential factor for gene expression and morphogenesis of the Xenopus gastrula embryo

Cellular dynamics and molecular control of the development of organizer-derived cells in quail-chick chimeras

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