Note: Extensive 16S rRNA gene sequence diversity in Campylobacter hyointestinalis strains: Taxonomic and applied implications

Harrington, Clare S.; On, Stephen L. W.

doi:10.1099/00207713-49-3-1171

Cited by 65 publications

(50 citation statements)

References 21 publications

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“…hyointestinalis subsp. lawsonii is possible (Harrington and On 1999). Although the 16S rRNA gene sequence of Camp.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of numerical analysis of PFGE-DNA profiles for differentiating Campylobacter fetus subspecies by comparison with phenotypic, PCR and 16S rDNA sequencing methods

On¹,

Harrington²

2001

J Appl Microbiol

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Aims: To assess the ef®cacy of numerical analysis of PFGE-DNA pro®les for identi®cation and differentiation of Campylobacter fetus subspecies. Methods and Results: 31 Camp. fetus strains were examined by phenotypic, PCR-and PFGE-based methods, and the 16S rDNA sequences of 18 strains compared. Numerical analysis of PFGE-DNA pro®les divided strains into two clusters at the 86% similarity level. One cluster contained 19 strains clearly identi®ed as Camp. fetus subsp. venerealis. The other cluster comprised 12 strains, of which 10 were unambiguously identi®ed as Camp. fetus subsp. fetus. The remaining two strains were identi®ed as Camp. fetus subsp. venerealis by either phenotypic or PCR methods, but not both. At higher similarity levels, clusters containing isolates from each of two countries were identi®ed, suggesting that certain clones predominate in certain geographical regions. Conclusions: Numerical analysis of PFGE-DNA pro®les is an effective method for differentiating Camp. fetus subspecies. Signi®cance and Impact of the Study: Critical comparison of PFGE, PCR, 16S rDNA sequencing and phenotypic methods for differentiation of Camp. fetus subspecies was attained. Novel phenotypic markers for distinguishing subspecies were identi®ed. Evidence for dominant clones of each subspecies in certain countries was provided.

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“…hyointestinalis subsp. lawsonii is possible (Harrington and On 1999). Although the 16S rRNA gene sequence of Camp.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of numerical analysis of PFGE-DNA profiles for differentiating Campylobacter fetus subspecies by comparison with phenotypic, PCR and 16S rDNA sequencing methods

On¹,

Harrington²

2001

J Appl Microbiol

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“…lawsonii isolates exceeded 99.9% and among C. intestinalis subsp. hyointestinalis isolates ranged from 96.4 to 100% [58]. Sequence identities between isolates representing the two subspecies ranged from 95.7 to 99.0%.…”

Section: Atypical Campylobactersmentioning

confidence: 99%

“…Surprisingly, an interventing sequence was identified in the C. hyointestinalis subsp. lawsonii strains [58]. AFLP fingerprinting method was also demonstrated to allow the classification of the C. hyointestinalis at the subspecies level [35].…”

Section: Atypical Campylobactersmentioning

confidence: 99%

Campylobacter

et al. 2005

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-Species within the genus, Campylobacter, have emerged over the last three decades as significant clinical pathogens, particularly of human public health concern, where the majority of acute bacterial enteritis in the Western world is due to these organisms. Of particular concern are the species, C. jejuni and C. coli, which are responsible for most of these gastrointestinal-related infections. Although these organisms have already emerged as causative agents of zoonoses, several aspects of their epidemiology and pathophysiology are only beginning to emerge. Trends in increasing antibiotic resistance are beginning to emerge with oral antibiotics, which may be the drug of choice for when it is necessary to intervene chemotherapeutically. This review wishes to examine (i) emerging clinical aspects of the disease, such as Guillain Barré syndrome (GBS), (ii) the association between these organisms and poultry as a natural host, (iii) environmental aspects of Campylobacter epidemiology, (iv) the emergence of atypical campylobacters (v) emerging trends in antibiotic resistance, (vi) adoption of modern methods for the detection of campylobacters.

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“…However, this tool has limitations due to the low polymorphism of 16S rRNA gene sequence data and high intraspecies diversity in some cases (Harrington & On, 1999;On, 2001). Therefore, additional genetic tools are needed to clarify the taxonomy of the genus Campylobacter and, at the same time, to enable the genetic identification of bacteria belonging to this genus.…”

mentioning

confidence: 99%

“…Numerical analysis of amplified fragment length polymorphism (AFLP) profiles has been used for the investigation of the genetic relationships within the genus Campylobacter (On & Harrington, 2000). In other studies, 16S rRNA and 23S rRNA gene sequence-based phylogenies within the genus were analysed (Van Camp et al, 1993;Harrington & On, 1999;Gorkiewicz et al, 2003). Kärenlampi et al (2004) performed phylogenetic analysis within the genus Campylobacter using partial sequences of the heat shock protein gene groEL.…”

mentioning

confidence: 99%

Genetic relatedness within the genus Campylobacter inferred from rpoB sequences

Korczak

Stieber

Emler

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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The genus Campylobacter comprises 17 species, some of which are important animal and human pathogens. To gain more insight into the genetic relatedness of this genus and to improve the molecular tools available for diagnosis, a universal sequencing approach was established for the gene encoding the beta-subunit of RNA polymerase (rpoB) for the genus Campylobacter. A total of 59 strains, including the type strains of currently recognized species as well as field isolates, were investigated in the study. A primer set specific for Campylobacter species enabled straightforward amplification and sequencing of a 530 bp fragment of the rpoB gene. The 16S rRNA gene sequences of all of the strains were determined in parallel. A good congruence was obtained between 16S rRNA and rpoB gene sequence-based trees within the genus Campylobacter. The branching of the rpoB tree was similar to that of the 16S rRNA gene tree, even though a few discrepancies were observed for certain species. The resolution of the rpoB gene within the genus Campylobacter was generally much higher than that of the 16S rRNA gene sequence, resulting in a clear separation of most species and even some subspecies. The universally applicable amplification and sequencing approach for partial rpoB gene sequence determination provides a powerful tool for DNA sequence-based discrimination of Campylobacter species.The genus Campylobacter was first proposed by M. Sebald and M. Véron in 1963 and included only the type species Campylobacter fetus and Campylobacter bubulus, now known as Campylobacter sputorum (Sebald & Véron, 1963). These taxa had formerly been classified as Vibrio species. In 1973, M. Véron and R. Chatelain included several misclassified Vibrio in the distinct Campylobacter genus based on serological, biochemical and DNA-DNA hybridization investigations (On, 2001). Since then, the taxonomy of the genus has changed dramatically. At present, it comprises 17 species with validly published names and six recognized subspecies (On, 2001;Foster et al., 2004). In addition, strains belonging to C. sputorum are divided into three biovars, sputorum, fecalis and paraureolyticus, on the basis of their ability to produce catalase or urease Vandamme & On, 2001).In general, members of the genus Campylobacter colonize the mucosal surfaces of the intestinal tract, oral cavity or urogenital tract of healthy, as well as diseased, humans and animals, especially birds. Several species may act as pathogens, causing disease in both human and animal hosts. Twelve of the 17 Campylobacter species are associated with human diseases. Campylobacter jejuni and Campylobacter coli are particularly frequent causative agents of human bacterial intestinal disorders worldwide (Skirrow, 1994). There have also been reported cases of diarrhoea in man caused by Campylobacter upsaliensis and Campylobacter lari, but the frequency of these infections is very low (Bourke et al., 1998;Van Doorn et al., 1998). Occasionally, Campylobacter species are implicated as causative agents of pericard...

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Note: Extensive 16S rRNA gene sequence diversity in Campylobacter hyointestinalis strains: Taxonomic and applied implications

Cited by 65 publications

References 21 publications

Evaluation of numerical analysis of PFGE-DNA profiles for differentiating Campylobacter fetus subspecies by comparison with phenotypic, PCR and 16S rDNA sequencing methods

Evaluation of numerical analysis of PFGE-DNA profiles for differentiating Campylobacter fetus subspecies by comparison with phenotypic, PCR and 16S rDNA sequencing methods

Campylobacter

Genetic relatedness within the genus Campylobacter inferred from rpoB sequences

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