Novel Antibacterial Activity of Enterococcus faecium NM2 
 Isolated from Urine of Healthy People

Enan, Gamal; El-Didamon, Gamal; Mohamed, E. A.; Zakaria, Azza S.

doi:10.3923/ajaps.2014.66.78

Cited by 15 publications

(8 citation statements)

References 15 publications

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“…These natural antimicrobials are quite promising for food bio-preservation to reduce the need for antibiotics, control of microbial spoilage process, and could kill the resistant variants of bacteria that can survive in foods and limit the occurrence of new food-borne disease outbreaks caused by pathogenic bacteria [ 7 , 8 , 9 , 10 , 11 , 12 ]. Natural extracts are compounds with a wide structural diversity, representing an important source of new chemical compounds with a possibly significant antibacterial action against food-borne pathogens with powerful action against multidrug-resistant bacteria [ 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ]. The possible negative impact of such chemicals on the environment and human health may preclude synthetic agents’ use [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…The possible negative impact of such chemicals on the environment and human health may preclude synthetic agents’ use [ 19 ]. Therefore, novel antimicrobial agents from natural sources are highly required [ 4 , 15 , 17 , 20 ]. Many strategies have been suggested to improve proteins’ antimicrobial activities, including chemical modification, e.g., such as esterification [ 22 , 23 , 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

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Powerful Antibacterial Peptides from Egg Albumin Hydrolysates

Al-Mohammadi¹,

Osman

Enan

et al. 2020

Antibiotics

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Native egg albumin (NEA) was isolated from hen eggs and hydrolyzed by pepsin to produce hydrolyzed egg albumin (HEA). HEA was chemically characterized and screened for its antibacterial activity against 10 pathogenic bacteria (6 Gram (+) and 4 Gram (−)). The SDS-PAGE pattern of NEA showed molecular weights of hen egg albumin subunits ranging from 30 to 180 kDa. The highest intensive bands appeared at a molecular mass of about 50 and 97 kDa. Ultra-performance liquid chromatography (UPLC) of the peptic HEA revealed 44 peptides, 17 of them were dipeptides, and the other 27 fractions corresponded to bigger peptides (3–9 amino acids). The dipeptides and big peptides represented 26% and 74% of the total hydrolysate, respectively. The MIC of HEA was about 100 μg/L for Listeria monocytogenes, Bacillus cereus, Staphylococcus aureus, Salmonella typhimurium, Streptococcus pyogenes, and Klebsiella oxytoca and 150 μg/L for Pseudomonas aeruginosa, Bacillus subtilis, and Listeria ivanovii and 200 μg/L for Escherichia coli. L. monocytogenes was the most sensitive organism to HEA. Mixtures of HEA with antibiotics showed more significant antibacterial activity than individually using them. Transmission electron microscopy (TEM) revealed various signs of cellular deformation in the protein-treated bacteria. HEA may electrostatically and hydrophobically interact with the cell wall and cell membrane of the susceptible bacteria, engendering large pores and pore channels leading to cell wall and cell membrane disintegration. Higher cell permeability may, thus, occur, leading to cell emptiness, lysis, and finally death. Alternatively, no toxicity signs appeared when HEA was administrated to Wistar Albino rats as one single dose (2000, 5000 mg/kg body weight) or repeated daily dose (500 and 2500 mg/kg body weight/day) for 28 days to disclose the possible toxicity hazards. HEA did not produce any death.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Powerful Antibacterial Peptides from Egg Albumin Hydrolysates

Al-Mohammadi¹,

Osman

Enan

et al. 2020

Antibiotics

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“…In addition to bacteria, kefir contains many yeast species such as Saccharomyces cerevisiae , Saccharomyces turensis , Saccharomyces fragilis , Candida kefyr , Kluveromyces marixians [ 62 ]. Certain kefir types do not need to contain all the above microorganisms, but the kefir content of microbiota differs from one type to another and this depends on many factors such as fermentation liquid, kefir grains used, sterilization conditions [ 63 ].…”

Section: Discussionmentioning

confidence: 99%

Chemical Constitution and Antimicrobial Activity of Kefir Fermented Beverage

Al-Mohammadi¹,

Ibrahim

Moustafa

et al. 2021

Molecules

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Kefir beverage (KB) is a fermented milk initiated by kefir grains rich with starter probiotics. The KB produced in this study seemed to contain many chemical compounds elucidated by gas chromatography–mass spectrometry (GC-MS) and IR spectra. These compounds could be classified into different chemical groups such as alcohols, phenols, esters, fatty esters, unsaturated fatty esters, steroids, polyalkenes, heterocyclic compounds and aromatic aldehydes. Both KB and neutralized kefir beverage (NKB) inhibited some pathogenic bacteria including Escherichia coli ATCC11229 (E. coli), Listeria monocytogenes ATCC 4957 (L. monocytogenes), Bacillus cereus ATCC 14579 (B. cereus), Salmonella typhimurium ATCC 14028 (Sal. typhimurium) as well as some tested fungal strains such as Aspergillus flavus ATCC 16872 (A. flavus) and Aspergillus niger ATCC 20611 (A. niger), but the inhibitory activity of KB was more powerful than that obtained by NKB. It also appeared to contain four lactic acid bacteria species, one acetic acid bacterium and two yeast species. Finally, the KB inhibited distinctively both S. aureus and Sal. typhimurium bacteria in a brain heart infusion broth and in some Egyptian fruit juices, including those made with apples, guava, strawberries and tomatoes.

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“…Cell free supernatant has an antibacterial activity against urinary tract infection pathogens as it contains organic acids that decrease the pH where uropathogens can't survive (Sawa et al, 2009 andEnan et al, 2014). These acids may acidify cytoplasm of susceptibility bacteria leading to collapsing proton gradient which causes bacterial death (Tharmaraj & Shah, 2009).…”

Section: Ipm Ak Cro Rf Ctx Azm Fep Cfp Cn Amc E Ofx Levmentioning

confidence: 99%