Novel approach for discrimination of eosinophilic granulocytes and evaluation of their surface receptors in a multicolor fluorescent histological assessment

Bila, Galyna; Schneider, Martina; Peshkova, Svetlana; Krajnik, Bartosz; Беш, Л.В.; Lutsyk, А. D.; Matsyura, O.; Bilyy, Rostyslav

doi:10.15407/ubj92.02.099

Cited by 8 publications

(6 citation statements)

References 43 publications

(63 reference statements)

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“…Human Jurkat T cells were cultured according to common laboratory practice techniques. When working with human monocytes derived from blood of healthy volunteers we used common best practices and criteria approved at [ 2 , 3 ].…”

Section: Methodsmentioning

confidence: 99%

Neutrophils as Main Players of Immune Response towards Nondegradable Nanoparticles

et al. 2020

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Many nano/microparticles (n/µP), to which our body is exposed, have no physiological way of removal. Our immune system sense these “small particulate objects”, and tries to decrease their harmfulness. Since oxidation, phagocytosis and other methods of degradation do not work with small, chemically resistant, and hydrophobic nanoparticles (nP). This applies to soot from air pollution, nano-diamonds from cosmic impact, polishing and related machines, synthetic polymers, and dietary n/µP. Our body tries to separate these from the surrounding tissue using aggregates from neutrophil extracellular traps (NETs). This effectively works in soft tissues where n/µP are entrapped into granuloma-like structures and isolated. The interactions of hydrophobic nanocrystals with circulating or ductal patrolling neutrophils and the consequent formation of occlusive aggregated NETs (aggNETs) are prone to obstruct capillaries, bile ducts in gallbladder and liver, and many more tubular structures. This may cause serious health problems and often fatality. Here we describe how specific size and surface properties of n/µP can activate neutrophils and lead to aggregation-related pathologies. We discuss “natural” sources of n/µP and those tightly connected to unhealthy diets.

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Section: Methodsmentioning

confidence: 99%

Neutrophils as Main Players of Immune Response towards Nondegradable Nanoparticles

et al. 2020

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“…24 h later, mice were sacrificed, and the air pouch membranes were analyzed. Cells of air pouch were analyzed using fluorescent microscopy as described in [ 48 ].…”

Section: Methodsmentioning

confidence: 99%

Rapid Generation of Coronaviral Immunity Using Recombinant Peptide Modified Nanodiamonds

et al. 2021

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Vaccination remains one of the most effective tools to prevent infectious diseases. To ensure that the best possible antigenic components are chosen to stimulate a cognitive immune response, boosting antigen presentation using adjuvants is common practice. Nanodiamond-based adjuvants are proposed here as a rapid and versatile platform for antigen conjugation, utilizing peptides common to different pathogenic strains and making this strategy a good candidate for a “ready-to-use” vaccine. Initiation of an inflammatory reaction with a resulting immune response is based on the ability of living organisms to entrap nanostructures such as nanodiamonds with neutrophil extracellular traps (NETs) formation. In this work, coronavirus peptide homological for MERS-CoV, fusion inhibitor, was conjugated to nanodiamonds and used to induce neutrophilic-driven self-limiting inflammation. The resulting adjuvant was safe and did not induce any tissue damage at the site of injection. Mice immunization resulted in IgG titers of ¼,000 within 28 days. Immunization of rabbits resulted in the formation of a high level of antibodies persistently present for up to 120 days after the first immunization (animal lifespan ~3 years). The peptide used for immunization proved to be reactive with sera of convalescent COVID patients, demonstrating the possibility of developing pancoronaviral vaccine candidates.

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“…Interesting results were obtained by Yadav et al, who studied the predisposition to allergic rhinitis [ 31 ]. A molecular genetic study of the IL-13 R130Q (rs20541) locus polymorphism showed a significantly increased rate of the GA genotype and A allele in patients with allergic rhinitis, in contrast to healthy people in the control group.…”

Section: Discussionmentioning

confidence: 99%

Polymorphic Variants of Interleukin-13 R130Q and Interleukin-4 T589C in Children with and without Cow’s Milk Allergy

Matsyura

Беш

Kens

et al. 2022

Life

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Cow’s milk allergy (CMA) is one of the most frequent types of food allergy. The aim of the study was to investigate whether IL-13 R130Q and IL-4 T589C polymorphisms are associated with the risk of CMA in young Ukrainian children. A total of 120 children (age range: 1–3 years) participated in the study and were divided into two groups: CMA (n = 60) and healthy controls (CNT, n = 60). The CMA group had two subgroups: specific oral tolerance induction (SOTI, n = 30) and milk elimination diet (MED, n = 30). The CNT group had two subgroups: positive family history of allergy (+FHA, n = 24) and negative family history of allergy (−FHA, n = 36). In the CMA group, molecular genetic testing of CC, CT, and TT genotypes of single nucleotide IL-4 T589C gene polymorphisms showed significantly higher rates of the CC genotype compared to healthy controls (92.2% vs. 58.8%; p < 0.01). In the CMA group, molecular genetic testing of GG, GA, and AA genotypes of single nucleotide IL-13 R130Q gene polymorphisms showed significantly higher rates of GA and AA polymorphic locus genotypes compared to healthy controls (43.5% vs. 22.4%, p < 0.05 and 8.7% vs. 0%, p < 0.05, respectively). In future studies, the genotypic and allelic distribution of these polymorphic variants will be determined in children with CMA and healthy children.

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Novel approach for discrimination of eosinophilic granulocytes and evaluation of their surface receptors in a multicolor fluorescent histological assessment

Cited by 8 publications

References 43 publications

Neutrophils as Main Players of Immune Response towards Nondegradable Nanoparticles

Neutrophils as Main Players of Immune Response towards Nondegradable Nanoparticles

Rapid Generation of Coronaviral Immunity Using Recombinant Peptide Modified Nanodiamonds

Polymorphic Variants of Interleukin-13 R130Q and Interleukin-4 T589C in Children with and without Cow’s Milk Allergy

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